MELANIN FORMATION BY HUMAN BRAIN IN VITRO

Abstract— A quantitative radiometric assay utilising incorporation of 14C from labelled precursors as a measure of melanin formation by human brain in vitro is described. The assay was validated by comparison with various criteria of melanin formation.

[1]  A. Oikawa,et al.  Quantitative measurement of melanin as tyrosine equivalents and as weight of purified melanin. , 1973, The Yale journal of biology and medicine.

[2]  M. Okun,et al.  Subcellular demonstration of peroxidatic oxidation of tyrosine to melanin using dihydroxyfumarate as cofactor in mouse melanoma cells. , 1973, The Journal of investigative dermatology.

[3]  M. Seiji,et al.  Tyrosinase in melanized melanosomes. , 1972, The Tohoku journal of experimental medicine.

[4]  L Stein,et al.  Possible Etiology of Schizophrenia: Progressive Damage to the Noradrenergic Reward System by 6-Hydroxydopamine , 1971, Science.

[5]  H. Barden.  THE HISTOCHEMICAL RELATIONSHIP OF NEUROMELANIN AND LIPOFUSCIN , 1969, Journal of neuropathology and experimental neurology.

[6]  U. Ungerstedt,et al.  6-Hydroxy-dopamine induced degeneration of central monoamine neurons. , 1968, European journal of pharmacology.

[7]  G. Fenichel,et al.  Studies on neuromelanin: II. Melanin in the brainstems of infants and children , 1968, Neurology.

[8]  K. Prasad,et al.  SPECTROSCOPIC STUDIES OF SUBSTANTIA NIGRA PIGMENT IN HUMAN SUBJECTS * , 1967, Journal of neurochemistry.

[9]  G. Fenichel,et al.  Studies on neuromelanin , 1967, Neurology.

[10]  H. Moses,et al.  Light and electron microscopic studies of pigment in human and rhesus monkey substantia nigra and locus coeruleus , 1966, The Anatomical record.

[11]  W. Whisler,et al.  Detection and study of a cyclization step preceding the formation of a fluorescent product in tyrosinase-catalyzed adrenaline oxidation. , 1966, Archives of biochemistry and biophysics.

[12]  Y. M. Chen,et al.  Radiometric assay of tyrosinase and theoretical considerations of melanin formation. , 1965, Analytical biochemistry.

[13]  D. Marsden.  BRAIN PIGMENT AND ITS RELATION TO BRAIN CATECHOLAMINES. , 1965, Lancet.

[14]  K. Kleppe,et al.  D-AMINO ACID OXIDASE. I. DISSOCIATION AND RECOMBINATION OF THE HOLOENZYME. , 1965, Biochimica et biophysica acta.

[15]  S. Pomerantz.  Separation, purification, and properties of two tyrosinases from hamster melanoma. , 1963, The Journal of biological chemistry.

[16]  M. T. Spoerlein,et al.  Oxidation of dopamine to melanin by an enzyme of rat brain. , 1963, Life sciences.

[17]  W. Harrison.  Detection of intermediate oxidation states of adrenaline and noradrenaline by fluorescence spectrometric analysis. , 1963, Archives of biochemistry and biophysics.

[18]  A. Duncan,et al.  Infra-Red Spectra of Some Melanins , 1962, Nature.

[19]  M. Rolland,et al.  New Intermediate of Melanogenesis in vitro , 1962, Nature.

[20]  Marsden Cd.  Pigmentation in the nucleus substantiae nigrae of mammals. , 1961 .

[21]  S. Udenfriend,et al.  Chemical, Enzymatic and Metabolic Studies on the Mechanism of Oxidation of Dopamine1 , 1959 .

[22]  J. Foley,et al.  ON THE NATURE OF PIGMENT GRANULES IN THE CELLS OF THE LOCUS COERULEUS AND SUBSTANTIA NIGRA , 1958, Journal of neuropathology and experimental neurology.

[23]  E. Margoliash,et al.  A study of the inhibition of catalase by 3-amino-1:2:4:-triazole. , 1958, The Biochemical journal.

[24]  J. Fellman.  EPINEPHRINE METABOLITES AND PIGMENTATION IN THE CENTRAL NERVOUS SYSTEM IN A CASE OF PHENYLPYRUVIC OLIGOPHRENIA , 1958, Journal of neurology, neurosurgery, and psychiatry.

[25]  K. Hellmann,et al.  Pigment formation from tryptamine and 5‐hydroxytryptamine in tissues: a contribution to the histochemistry of amine oxidase , 1953, The Journal of physiology.

[26]  H. S. Mason.  The Structure of Melanins , 1953 .

[27]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[28]  T. Fitzpatrick,et al.  Mammalian tyrosinase; preparation and properties. , 1949, The Journal of biological chemistry.