Selective determination of epinephrine in the presence of ascorbic acid and uric acid by electrocatalytic oxidation at poly(eriochrome black T) film-modified glassy carbon electrode.

An electropolymerized film of eriochrome black T (EBT) has been prepared at a glassy carbon electrode (GCE) by cyclic voltammetry (CV). The poly(EBT) membrane at GCE exhibits an excellent electrocatalytic activity towards the oxidation of epinephrine (EP), ascorbic acid (AA) and uric acid (UA) in acidic solution and reduced the overpotential for the oxidation of EP. The poly(EBT)-coated electrode could separately detect EP, AA and UA in their mixture with the potential differences of 180 and 160 mV for EP-AA and UA-EP, respectively, which are large enough to allow for determination of EP in the presence of AA and UA. Using differential pulse voltammetry, the peak current of EP recorded in pH 3.5 solution was linearly dependent on EP's concentration in the range of 2.5 - 50 microM. Due to its good selectivity and stability, the polymer-coated GCE was successfully applied to the determination of EP in real samples.

[1]  Xiuhua Zhang,et al.  Simultaneous determination of epinephrine and ascorbic acid at the electrochemical sensor of triazole SAM modified gold electrode , 2006 .

[2]  Myung-Hoon Kim,et al.  Anodic square-wave stripping voltammetric analysis of epinephrine using carbon fiber microelectrode , 2006 .

[3]  Protiva Rani Roy,et al.  Simultaneous electroanalysis of dopamine and ascorbic acid using poly (N,N-dimethylaniline)-modified electrodes. , 2003, Bioelectrochemistry.

[4]  R. Yu,et al.  Permselectivity of neurotransmitters at overoxidized polypyrrole-film-coated glassy carbon electrodes. , 1996, Talanta.

[5]  Hongyuan Chen,et al.  Amperometric determination of epinephrine with an osmium complex and Nafion double-layer membrane modified electrode , 1999 .

[6]  H. Luo,et al.  Simultaneous voltammetric measurement of ascorbic acid, epinephrine and uric acid at a glassy carbon electrode modified with caffeic acid. , 2006, Biosensors & bioelectronics.

[7]  Xinhua Lin,et al.  Flow-injection chemiluminescence determination of catecholamines based on their enhancing effects on the luminol-potassium periodate system. , 2006, Luminescence : the journal of biological and chemical luminescence.

[8]  Shen-ming Chen,et al.  Preparation, characterization and electrocatalytic properties of poly(luminol) and polyoxometalate hybrid film modified electrodes , 2005 .

[9]  B. Ye,et al.  Simultaneous determination of dopamine and ascorbic acid at poly(neutral red) modified electrodes , 1998 .

[10]  P Nagaraja,et al.  A sensitive and selective spectrophotometric estimation of catechol derivatives in pharmaceutical preparations. , 2001, Talanta.

[11]  Shen-ming Chen,et al.  The electrochemical properties of dopamine, epinephrine, norepinephrine, and their electrocatalytic reactions on cobalt(II) hexacyanoferrate films , 2003 .

[12]  Ren-min Liu,et al.  Study on the electrochemical behavior of epinephrine at a poly(3-methylthiophene)-modified glassy carbon electrode , 2004 .

[13]  Dan Du,et al.  Electrochemical behavior of epinephrine at l-cysteine self-assembled monolayers modified gold electrode. , 2002, Talanta.

[14]  R. Ramaraj,et al.  Simultaneous determination of ascorbic acid, dopamine and serotonin at poly(phenosafranine) modified electrode , 2003 .

[15]  Shen-ming Chen,et al.  Electrochemical self-assembly of nordihydroguaiaretic acid/Nafion modified electrodes and their electrocatalytic properties for catecholamines and ascorbic acid , 2005 .

[16]  Lijun He,et al.  Effect of Ionic Liquids as Mobile Phase Additives on Retention of Catecholamines in Reversed-Phase High-Performance Liquid Chromatography , 2003 .

[17]  D. Barrett,et al.  Catecholamines derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole: characterization of chemical structure and fluorescence properties , 2003 .

[18]  Shen-ming Chen,et al.  Preparation and characterization of osmium hexacyanoferrate films and their electrocatalytic properties , 2004 .

[19]  Shen-ming Chen,et al.  Preparation and electrocatalytic properties of osmium oxide/hexacyanoruthenate films modified electrodes for catecholamines and sulfur oxoanions , 2006 .

[20]  Xiangqin Lin,et al.  A glassy carbon supported bilayer lipid-like membrane of 5,5-ditetradecyl-2-(2-trimethyl-ammonioethyl)-1,3-dioxane bromide for electrochemical sensing of epinephrine , 2004 .