Oxidant/Antioxidant Status of Patients with Diabetic and Senile Cataract

ABSTRACT The aim of the present study is to evaluate the relationship of total antioxidant capacity, total oxidant status, oxidative stres and total free sulfydryl groups in patients with diabetic and nondiabetic senil cataract. The study was carried out on venous blood samples from 70 subjects admitted to the ophtalmology clinic of a research hospital. Of these, 25 were senile Type 2 diabetic patients with cataract who were using oral hypoglycemic agents or insülin. Fourty-five patients with nondiabetic senile cataract comprised the control group. Total oxidant status (TOS), total antioxidant capacity (TAC) and total free -SH levels were measured in sera of patients with cataract. Serum TAS and TOS levels were determined using a novel automated measurement method. Oxidative status was determined using TAC and TOS measurement and with the calculation of oxidative stres index. TOS and OSI levels were significantly higher in diabetic cataract than senile cataract (p=0.008, p=0.013), respectively. TAC and total free -SH levels were similiar in the two groups (p = 0.17, p=0.54). Free sulfydryl groups levels are consistent with TAC. The results of present study suggest that TOS and intensity of the oxidative stress in diabetic patients are higher, compared to other age-related cataract patients without diabetes. Since oxidative stress is important in development of diabetic complications, increased TOS and oxidative stress might have a role in the pathophysiology of diabetic cataract.

[1]  Filiz Yildirim,et al.  The Evaluation of the Oxidative Stress Parameters in Nondiabetic and Diabetic Senile Cataract Patients , 2009, Biological Trace Element Research.

[2]  A. Gul,et al.  Antioxidant status in diabetic and non-diabetic senile patients, with cataract or cardiovascular complications. , 2008, Saudi medical journal.

[3]  Anjuman Gul,et al.  Could Oxidative Stress Associate with Age Products in Cataractogenesis? , 2008, Current eye research.

[4]  P. S. Murthy,et al.  Blood lipid peroxidation products and antioxidants in senile cataract , 1996, Indian Journal of Clinical Biochemistry.

[5]  A. Ohira,et al.  [Oxidative stress in ocular disease]. , 2008, Nippon Ganka Gakkai zasshi.

[6]  H. Çelik,et al.  Relationship between total oxidant status and severity of diabetic nephropathy in type 2 diabetic patients. , 2007, Nutrition, metabolism, and cardiovascular diseases : NMCD.

[7]  E. Araki,et al.  Impact of mitochondrial ROS production in the pathogenesis of insulin resistance. , 2007, Diabetes research and clinical practice.

[8]  Poornima Sharma,et al.  Glyceryl trinitrate spray in the management of painful diabetic neuropathy: a randomized double blind placebo controlled cross-over study. , 2007, Diabetes research and clinical practice.

[9]  K. Tsubota.  [Oxidative stress and inflammation: hypothesis for the mechanism of aging]. , 2007, Nippon Ganka Gakkai zasshi.

[10]  J. Patel.  Is cataract surgery a risk factor for progression of macular degeneration? , 2007, Current opinion in ophthalmology.

[11]  M. Číž,et al.  Alterations in plasma antioxidants during reperfusion of the ischemic small intestine in rats. , 2006, Research in veterinary science.

[12]  J. Vinson.  Oxidative stress in cataracts. , 2006, Pathophysiology : the official journal of the International Society for Pathophysiology.

[13]  O. Erel,et al.  Measuring plasma oxidative stress biomarkers in sport medicine , 2006, European Journal of Applied Physiology.

[14]  Elena Ganea,et al.  Glutathione-Related Enzymes and the Eye , 2006, Current eye research.

[15]  O. Erel,et al.  A new automated colorimetric method for measuring total oxidant status. , 2005, Clinical biochemistry.

[16]  M. Kantorow,et al.  Molecular genetics of age-related cataract. , 2004, Experimental eye research.

[17]  Z. Kyselova,et al.  Pharmacological prevention of diabetic cataract. , 2004, Journal of diabetes and its complications.

[18]  Sujata Joshi,et al.  Lycopene attenuates oxidative stress induced experimental cataract development: an in vitro and in vivo study. , 2003, Nutrition.

[19]  A. Carrascosa,et al.  Biomarkers of diabetes-associated oxidative stress and antioxidant status in young diabetic patients with or without subclinical complications. , 2003, Free radical biology & medicine.

[20]  Guoyao Wu,et al.  Free radicals, antioxidants, and nutrition. , 2002, Nutrition.

[21]  L. Włodek.  Beneficial and harmful effects of thiols. , 2002, Polish journal of pharmacology.

[22]  T. Oshika,et al.  Glycemic control and lens transparency in patients with type 1 diabetes mellitus. , 2001, American journal of ophthalmology.

[23]  F. Akçay,et al.  Diabetic Cataract and the Total Antioxidant Status in Aqueous Humor , 2001, Clinical chemistry and laboratory medicine.

[24]  A. Cerami,et al.  Protein glycation, diabetes, and aging. , 2001, Recent progress in hormone research.

[25]  F. Boscia,et al.  Protein oxidation and lens opacity in humans. , 2000, Investigative ophthalmology & visual science.

[26]  D. Cucinotta,et al.  Age and metabolic control influence lens opacity in type I, insulin-dependent diabetic patients. , 1999, Journal of diabetes and its complications.

[27]  L. Laatikainen,et al.  Diabetic cataract in children. , 1998, Acta ophthalmologica Scandinavica.

[28]  B. Özmen,et al.  Lens Lipid Peroxides and Glutathione Concentrations in Diabetic Cataract , 1997, Annals of clinical biochemistry.

[29]  Y. Obara.  [The oxidative stress in the cataract formation]. , 1995, Nippon Ganka Gakkai zasshi.

[30]  R. Klein,et al.  Incidence of cataract surgery in the Wisconsin Epidemiologic Study of Diabetic Retinopathy. , 1995, American journal of ophthalmology-glaucoma.

[31]  J. Nyengaard,et al.  Hyperglycemic Pseudohypoxia and Diabetic Complications , 1993, Diabetes.

[32]  C. Cross,et al.  Antioxidant protection against hypochlorous acid in human plasma. , 1993, The Journal of laboratory and clinical medicine.

[33]  J. G. Scandalios.  Molecular biology of free radical scavenging systems , 1992 .

[34]  M. Sanaka,et al.  Effect of diabetes on the free polyol pattern in cataractous lenses. , 1991, Clinical chemistry.

[35]  T. Cosgrove,et al.  Reaction of xanthine oxidase-derived oxidants with lipid and protein of human plasma. , 1991, Archives of biochemistry and biophysics.

[36]  B. Ames,et al.  Antioxidant defenses and lipid peroxidation in human blood plasma. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[37]  Z. Pollard.  Bilateral superior oblique muscle palsy associated with Apert's syndrome. , 1988, American journal of ophthalmology.

[38]  L. Chylack,et al.  Antioxidant status in persons with and without senile cataract. , 1988, Archives of ophthalmology.

[39]  K. Ingold,et al.  The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. , 1987, Biochimica et biophysica acta.

[40]  A. Bron,et al.  Cataract and retinopathy: screening for treatable retinopathy. , 1986, Clinics in endocrinology and metabolism.

[41]  B. Halliwell,et al.  Free radicals in biology and medicine , 1985 .

[42]  V. M. Sorokin,et al.  [Fractionation of the venom of the cobra of central Asia on sulfoethylsephadex and a study of the biological activity of the fractions obtained]. , 1972, Biokhimiia.

[43]  G. Ellman,et al.  Tissue sulfhydryl groups. , 1959, Archives of biochemistry and biophysics.