Quantitation of hepatitis B viral DNA by solution hybridization: Comparison with DNA polymerase and hepatitis B e antigen during antiviral therapy

Serological markers of hepatitis B virus (HBV) replication were assessed in a randomized, controlled trial of prednisone withdrawal followed by α ‐interferon in the treatment of chronic hepatitis B. HBV DNA levels in more than 700 serial serum samples from 41 patients were determined by a sensitive and quantitative solution hybridization assay. Results were compared with HBV DNA polymerase (DNAp) activity and hepatitis B e antigen (HBeAg) in 21 untreated controls and 20 treated patients.

[1]  M. Peters,et al.  Prednisone withdrawal followed by recombinant alpha interferon in the treatment of chronic type B hepatitis. A randomized, controlled trial. , 1988, Annals of internal medicine.

[2]  H. Varmus,et al.  The molecular biology of the hepatitis B viruses. , 1987, Annual review of biochemistry.

[3]  J. Hoofnagle,et al.  Immunologic effects of interferon-alpha in man: treatment with human recombinant interferon-alpha suppresses in vitro immunoglobulin production in patients with chronic type B hepatitis. , 1986, Journal of immunology.

[4]  S. Ida,et al.  Determination of Hepatitis B Virus DNA in Serum by Molecular Hybridization , 1984, Microbiology and immunology.

[5]  D. Bradley,et al.  DNA: DNA hybridization method for the diagnosis of hepatitis B infection. , 1984, Journal of virological methods.

[6]  D. Shafritz,et al.  Detection of Hepatitis B Virus DNA Directly in Human Serum by a Simplified Molecular Hybridization Test: Comparison to HBeAg/ Anti‐HBe Status in HBsAg Carriers , 2007, Hepatology.

[7]  C. Bréchot,et al.  Detection of Hepatitis B Virus DNA in Serum by a Simple Spot Hybridization Technique: Comparison with Results for Other Viral Markers , 2007, Hepatology.

[8]  S. Sherlock,et al.  Relationship between HBV-specific DNA polymerase and HBe antigen/antibody system in chronic HBV infection: factors determining selection of patients and outcome of antiviral therapy. , 1983, Gut.

[9]  H. Thomas,et al.  The detection of hbv‐dna in serum by molecular hybridisation: A more sensitive method for the detection of complete hbv particles , 1982, Journal of medical virology.

[10]  J. Gerin,et al.  An assay for the detection of the dna genome of hepatitis b virus in serum , 1982, Journal of Medical Virology.

[11]  C. Bréchot,et al.  DETECTION OF HEPATITIS B VIRUS DNA IN LIVER AND SERUM: A DIRECT APPRAISAL OF THE CHRONIC CARRIER STATE , 1981, The Lancet.

[12]  J. Gerin,et al.  Hepatitis B virus DNA in the sera of HBsAg carriers: A marker of active hepatitis B virus replication in the liver , 1981, Hepatology.

[13]  R. Greenman,et al.  DNA Polymerase Associated with Human Hepatitis B Antigen , 1973, Journal of virology.