THE EFFECT OF GLUCOCORTICOSTEROIDS ON THE PROLIFERATION AND KINETICS OF PROMONOCYTES AND MONOCYTES OF THE BONE MARROW

To elucidate mechanisms underlying the prolonged monocytopenia induced in the peripheral blood of mice by injection of a subcutaneous depot of hydrocortisone acetate, the effect of this compound on the production of monocytes and their release from the bone marrow was studied. Hydrocortisone was found to cause a rapid reduction of the bone marrow promonocytes to about 65% of their initial number. The number of monocytes in the bone marrow decreased gradually, over a period of 96 h, to 75% of the initial value. The mitotic activity of the promonocytes was not diminished, as judged from the labeling in vitro with [3H]thymidine and the DNA-synthesis and cell-cycle times of these cells. The production of monocytes was only moderately diminished, i.e., to about 80% of the normal amount. The release of monocytes from the bone marrow was found to be influenced by hydrocortisone. After in vivo labeling with [3H]thymidine the monocyte-labeling indices were initially significantly higher in hydrocortisone-treated than in normal mice. It is concluded that a decreased production of monocytes in the bone marrow cannot account for the prolonged monocytopenia in the peripheral blood after hydrocortisone administration. However, hydrocortisone interferes with the release of newly formed monocytes from the bone marrow, resulting in a prolonged sojourn of these cells in this compartment.

[1]  H. Blomgren,et al.  In vitro stimulation of mouse thymus cells by PHA and allogeneic cells. , 1971, Cellular immunology.

[2]  S. Killmann,et al.  Perturbation of generation cycle of human leukemic blast cells by cytostatic therapy in vivo: effect of corticosteroids. , 1970, Blood.

[3]  W. Bullough,et al.  The lymphocytic chalone and its antimitotic action on a mouse lymphoma in vitro. , 1970, European journal of cancer.

[4]  J. Cohen,et al.  Hydrocortisone Resistance of Graft vs Host Activity in Mouse Thymus, Spleen and Bone Marrow , 1970, The Journal of Immunology.

[5]  M. D. Diesselhoff-den Dulk,et al.  THE KINETICS OF PROMONOCYTES AND MONOCYTES IN THE BONE MARROW , 1970, The Journal of experimental medicine.

[6]  R. van Furth,et al.  MORPHOLOGY AND PEROXIDASE CYTOCHEMISTRY OF MOUSE PROMONOCYTES, MONOCYTES, AND MACROPHAGES , 1970, The Journal of experimental medicine.

[7]  H. Claman,et al.  Bone Marrow and Spleen: Dissociation of Immunologic Properties by Cortisone , 1970, Science.

[8]  R. van Furth,et al.  THE EFFECT OF GLUCOCORTICOSTEROIDS ON THE KINETICS OF MONONUCLEAR PHAGOCYTES , 1970, The Journal of experimental medicine.

[9]  D. Metcalf,et al.  Cortisone Action on Serum Colony-Stimulating Factor and Bone Marrow in Vitro Colony-Forming Cells∗ , 1969, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[10]  T. Rytömaa,et al.  THE ROLE OF STRESS HORMONES IN THE CONTROL OF GRANULOCYTE PRODUCTION , 1969 .

[11]  B. Lampkin,et al.  Drug effect in acute leukemia. , 1969, The Journal of clinical investigation.

[12]  Kollmorgen Gm The response of HeLa cells to irradiation and hydrocortisone. , 1969 .

[13]  R. van Furth,et al.  THE ORIGIN AND KINETICS OF MONONUCLEAR PHAGOCYTES , 1968, The Journal of experimental medicine.

[14]  G. Cartwright,et al.  Quantitative studies of blood and bone marrow neutrophils in normal mice. , 1968, The American journal of physiology.

[15]  W. Bullough,et al.  THE ROLE OF GLUCOCORTICOID HORMONES IN THE CONTROL OF EPIDERMAL MITOSIS , 1968 .

[16]  A. S. Gordon,et al.  Mechanisms of prednisolone action in erythropoiesis. , 1967, Endocrinology.

[17]  E. Reisner Tissue culture of bone marrow. II. Effect of steroid hormones on hematopoiesis in vitro. , 1966, Blood.

[18]  D. Berliner,et al.  Comparison of the growth of fibroblasts under the influence of 11-betahydroxy and 11-keto corticosteroids. , 1966, Endocrinology.

[19]  D. Berliner,et al.  EFFECT OF STEROIDS ON GROWTH OF MOUSE FIBROBLASTS IN VITRO. , 1965, Endocrinology.

[20]  A. Handler,et al.  EFFECT OF HIGH DOSES OF CORTISONE ON BONE MARROW CELL PROLIFERATION IN THE SYRIAN HAMSTER. , 1964, Cancer research.

[21]  P. Nowell Inhibition of human leukocyte mitosis by prednisolone in vitro. , 1961, Cancer research.

[22]  H. Florey,et al.  The influence of Cortisone on cell division. , 1952, Quarterly journal of experimental physiology and cognate medical sciences.

[23]  W. Bullough STRESS AND EPIDERMAL MITOTIC ACTIVITY. , 1952 .

[24]  J. M. Yoffey,et al.  Effect of Repeated Injections of A.C.T.H. Upon the Bone Marrow , 1952, British medical journal.

[25]  N. Wald,et al.  The effect of massive doses of cortisone on the peripheral blood and bone marrow of the mouse. , 1951, Blood.

[26]  G. Kollmorgen The response of HeLa cells to irradiation and hydrocortisone. , 1969, National Cancer Institute monograph.

[27]  W. Bullough,et al.  Mitotic Homeostasis , 1965, Nature.

[28]  W. Bullough Stress and epidermal mitotic activity. I. The effects of the adrenal hormones. , 1952, The Journal of endocrinology.