A tissue adhesives evaluated in vitro and in vivo analysis.

In this study, three kinds of two-component adhesive glues were prepared, namely, gel-dext glue made from modified gelatin and dextran, gel-HES glue made from modified gelatin and hydroxyethyl starch (HES), and chit-dext glue made from chitosan and modified dextran. Upon mixing the two-component solution together crosslinking occurred and a gel formed in several seconds, which would seal the wound tissue and stop the bleeding. The adhesive ability of those three prepared glues was evaluated in vitro and in vivo separately by measuring the bonding strength to two piece of porcine skin and the adhesive strength after sealing the skin incisions on the back of rat. Fibrin glue was used as comparing. Gel-dext glue and gel-HES glue shown higher bonding strength and adhesive strength than chit-dext glue and fibrin glue. Histology test of incision tissues given by both HE and MTC methods, the former shown that gel-dext and gel-HES glues, like fibrin glue, have only normal initial inflammation to skin tissue, which almost disappear from 9 days but chit-dext glue seams have heaver inflammation, which may last to 12 days; the later shown gel-dext and gel-HES glues similar to fibrin glue, can heal the wound fast than that of chit-dext glue. The hemostatic ability for gel-HES glue was also tested on a cut liver of rat, which depend on the gel formation speed when the two-composite solutions were mixed together. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.

[1]  O. Thetter.  Fibrin adhesive and its application in thoracic surgery. , 1981, The Thoracic and cardiovascular surgeon.

[2]  D. Chopin,et al.  Conservative treatment of renal allograft rupture with polyglactin 910 mesh and gelatin resorcin formaldehyde glue. , 1989, The Journal of urology.

[3]  Y Ikada,et al.  In vitro toxicity test of 2-cyanoacrylate polymers by cell culture method. , 1990, Journal of biomedical materials research.

[4]  B. Berthelsen,et al.  Embolization of Cerebral Arteriovenous Malformations with Bucrylate , 1990, Acta radiologica.

[5]  C. Laurian,et al.  Use of biological glue in acute aortic dissection. Preliminary clinical results with a new surgical technique. , 1979, The Journal of thoracic and cardiovascular surgery.

[6]  Y. Shimizu,et al.  A new type of surgical adhesive made from porcine collagen and polyglutamic acid. , 2001, Journal of biomedical materials research.

[7]  Y. Ikada,et al.  A novel surgical glue composed of gelatin and N-hydroxysuccinimide activated poly(L-glutamic acid): Part 1. Synthesis of activated poly(L-glutamic acid) and its gelation with gelatin. , 1998, Biomaterials.

[8]  Y. M. Lee,et al.  Studies on gelatin-containing artificial skin: II. Preparation and characterization of cross-linked gelatin-hyaluronate sponge. , 1999, Journal of biomedical materials research.

[9]  Y. Ikada,et al.  Soft tissue adhesive composed of modified gelatin and polysaccharides , 2000, Journal of biomaterials science. Polymer edition.

[10]  Y. Ikada,et al.  A new biological glue from gelatin and poly (L-glutamic acid). , 1996, Journal of biomedical materials research.

[11]  T. Neff,et al.  Pharmacokinetics of Hydroxyethyl Starch , 2005, Clinical pharmacokinetics.

[12]  J. Mano,et al.  Stimuli-responsive hydrogels based on polysaccharides incorporated with thermo-responsive polymers as novel biomaterials. , 2006, Macromolecular bioscience.