HNA-1a, HNA-1b and HNA-1c gene frequencies in Argentineans.

Human neutrophil antigens (HNA) are polymorphic structures located in the neutrophil membrane. The neutrophil-specific antigens HNA-1a (NA1), 1b (NA2) and 1c (SH) are well-recognized allotypic forms of FcgammaRIIIb and the most frequent targets of neutrophil alloantibodies. The aim of this study was to determine the gene frequencies of the neutrophil-specific antigens belonging to the HNA-1 system in blood donors and Toba Amerindians from Rosario, Argentina. Two hundred and eighteen unrelated healthy Argentinean blood donors and Toba Amerindians from Rosario were typed for HNA-1a, HNA-1b and HNA-1c using polymerase chain reaction with sequence-specific primers. For the Argentinean blood donors, the HNA-1a and HNA-1b gene frequencies were 0.44 and 0.56 and for the Amerindians Toba were 0.77 and 0.23, respectively. The HNA-1c antigen is present in 4.7% (gene frequency=0.023) of the blood donors but in none of the Amerindian individuals. The present data showed that the HNA-1 allele frequencies in the major population and the Toba Amerindians from Rosario are similar to those described in European and others distant Amerindians populations, respectively.

[1]  M. Zago,et al.  Variation in the FcgammaR3B gene among distinct Brazilian populations. , 2005, Tissue antigens.

[2]  R. Siebert,et al.  FCGR3 variants and expression of human neutrophil antigen‐1a, ‐1b, and ‐1c in the populations of northern Germany and Uganda , 2002, Transfusion.

[3]  M. Figueiredo,et al.  Allelic polymorphisms of human Fcγ receptor IIa and Fcγ receptor IIIb among distinct groups in Brazil , 2000 .

[4]  P. Tazzari,et al.  Human-platelet-antigen and neutrophil-antigen gene frequency in the Italian population determined by polymerase chain reaction with sequence specific primers. , 2000, Haematologica.

[5]  K. Tokunaga,et al.  Determination of Granulocyte‐Specific Antigens on Neutrophil Fcγ Receptor IIIb by PCR‐Preferential Homoduplex Formation Assay, and Gene Frequencies in the Japanese Population , 1999, Vox sanguinis.

[6]  R. Steffensen,et al.  FcγRIIIB polymorphism: evidence that NA1/NA2 and SH are located in two closely linked loci and that the SH allele is linked to the NA1 allele in the Danish population , 1999, Transfusion.

[7]  M. Álvarez-López,et al.  Determination of NA gene frequencies in the Spanish population by polymerase chain reaction with sequence-specific primers. , 1998, European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics.

[8]  P. Bierling,et al.  Characterization of a New Alloantigen (SH) on the Human Neutrophil Fcγreceptor IIIb , 1997 .

[9]  M. Hessner,et al.  Determination of neutrophil antigen gene frequencies in five ethnic groups by polymerase chain reaction with sequence‐specific primers , 1996, Transfusion.

[10]  C. Mueller-Eckhardt,et al.  NA gene frequencies in the German population, determined by polymerase chain reaction with sequence‐specific primers , 1995, Transfusion.