A simple and rapid method for isolating cassava leaf linamarase suitable for cassava cyanide determination

A simple method was developed for isolating cassava leaf linamarase. It involved homogenising the leaves in a bu†er containing polyvinylpolypyrroli- done, followed by ammonium sulphate precipitation, -ltration and partial puri-- cation of the enzyme by hydrophobic interaction chromatography. All stages of the enzyme preparation were carried out at room temperature and it was com- pleted within 90 min. A linamarin-indicator strip for checking the activity of the enzyme preparation was also developed. This isolation technique which has been developed into a simple kit should be suitable for laboratories in developing countries where the enzyme is needed to determine the cyanogenic potential of cassava and its products.

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