cDNA Cloning and Characterization of a Cek7 Receptor Protein-tyrosine Kinase Ligand That Is Identical to the Ligand (ELF-1) for the Mek-4 and Sek Receptor Protein-tyrosine Kinases (*)

We have isolated a murine cDNA encoding a ligand for the Cek7 receptor protein-tyrosine kinase (RPTK), a member of the Eph/Eck RPTK subfamily. Sequence analysis predicts an open reading frame of 209 amino acids with a predicted molecular mass of 24 kDa. The Cek7 ligand shows a 48% sequence identity at the protein level to B61, a ligand for the related Eck RPTK, 30% to the Cek5 ligand, 59% to the recently cloned Ehk1-L, and identity to ELF-1, a recently described ligand for the Mek4 and Sek RPTKs. The expressed Cek7 ligand is functionally active as it induces autophosphorylation of the Cek7 RPTK.

[1]  T Pawson,et al.  Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity. , 1994, Science.

[2]  A. Pandey,et al.  cDNA cloning and characterization of a ligand for the Cek5 receptor protein-tyrosine kinase. , 1994, The Journal of biological chemistry.

[3]  M. Verderame,et al.  Identification of a complete Cek7 receptor protein tyrosine kinase coding sequence and cDNAs of alternatively spliced transcripts. , 1994, Gene.

[4]  J. Flanagan,et al.  Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases , 1994, Cell.

[5]  T. Pawson,et al.  Molecular characterization of a family of ligands for eph‐related tyrosine kinase receptors. , 1994, The EMBO journal.

[6]  J. Lowe,et al.  Molecular cloning of a murine N-acetylgalactosamine transferase cDNA that determines expression of the T lymphocyte-specific CT oligosaccharide differentiation antigen. , 1994, The Journal of biological chemistry.

[7]  Yosef Yarden,et al.  B61 is a ligand for the ECK receptor protein-tyrosine kinase , 1994, Nature.

[8]  R Amthauer,et al.  Biosynthesis of glycosylphosphatidylinositol (GPI)-anchored membrane proteins in intact cells: specific amino acid requirements adjacent to the site of cleavage and GPI attachment , 1993, The Journal of cell biology.

[9]  M. Nieto,et al.  An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain. , 1992, Oncogene.

[10]  V. Dixit,et al.  The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity. , 1992, The Journal of biological chemistry.

[11]  S Udenfriend,et al.  Phosphatidylinositol glycan (PI-G) anchored membrane proteins. Amino acid requirements adjacent to the site of cleavage and PI-G attachment in the COOH-terminal signal peptide. , 1992, The Journal of biological chemistry.

[12]  M. Barbacid,et al.  The trkB tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3 , 1991, Cell.

[13]  T. Pawson,et al.  Characterization of elk, a brain-specific receptor tyrosine kinase , 1991, Molecular and cellular biology.

[14]  T. Hunter,et al.  cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases , 1990, Molecular and cellular biology.

[15]  V. Dixit,et al.  A novel immediate-early response gene of endothelium is induced by cytokines and encodes a secreted protein , 1990, Molecular and cellular biology.

[16]  I. Stamenkovic,et al.  CD44 is the principal cell surface receptor for hyaluronate , 1990, Cell.

[17]  R. Cummings,et al.  Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl- 1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[18]  M. Kozak.  The scanning model for translation: an update , 1989, The Journal of cell biology.

[19]  B. Seed,et al.  Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[20]  S. Munro,et al.  An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein , 1986, Cell.

[21]  G. Heijne.  A new method for predicting signal sequence cleavage sites. , 1986 .

[22]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.

[23]  B. Hirt.  Selective extraction of polyoma DNA from infected mouse cell cultures. , 1967, Journal of molecular biology.