Plasma membrane depolarization and calcium influx during cell injury by photodynamic action.

The plasma membrane has been implicated as one of the critical targets of photodynamic action. We observed that plasma membrane depolarization is an early event in the photodynamic action of zinc phthalocyanine disulfonate in mouse myeloma cells, showing both photosensitizer concentration and light dose dependence. The depolarization was observed immediately upon exposure to light, while membrane integrity was retained and showed a strong correlation with cell killing. In this study the use of channel blockers and alteration of ion concentration was employed to determine the factors involved in the membrane depolarization process. A general rise in cation permeability is associated with the depolarization. Loss of intracellular potassium was detected and an increase in intracellular free calcium was also observed. Sodium was found to strongly influence the photosensitized depolarization.

[1]  T. Dubbelman,et al.  Photodynamic effects of hematoporphyrin-derivative on enzyme activities of murine L929 fibroblasts. , 1987, Journal of photochemistry and photobiology. B, Biology.

[2]  John Pooler,et al.  Photodynamic Alteration of Sodium Currents in Lobster Axons , 1972, The Journal of general physiology.

[3]  J. Farber,et al.  Calcium dependence of toxic cell death: a final common pathway. , 1979, Science.

[4]  M. Rodgers,et al.  DEPOLARIZATION OF MOUSE MYELOMA CELL MEMBRANES DURING PHOTODYNAMIC ACTION , 1990, Photochemistry and photobiology.

[5]  C. Rabito,et al.  Oxygen radicals alter the cell membrane potential in a renal cell line (LLC-PK1) with differentiated characteristics of proximal tubular cells. , 1987, Biochimica et biophysica acta.

[6]  H. Tanke,et al.  PHOTODYNAMIC EFFECTS OF HEMATOPORPHYRIN DERIVATIVE ON THE UPTAKE OF RHODAMINE 123 BY MITOCHONDRIA OF INTACT MURINE L929 FIBROBLASTS AND CHINESE HAMSTER OVARY Kl CELLS , 1988, Photochemistry and photobiology.

[7]  K. Chandy,et al.  Two types of potassium channels in murine T lymphocytes , 1987, The Journal of general physiology.

[8]  K. Philipson,et al.  Effects of fatty acids on Na+-Ca2+ exchange and Ca2+ permeability of cardiac sarcolemmal vesicles. , 1985, The Journal of biological chemistry.

[9]  J. Pounds.  The role of cell calcium in current approaches to toxicology. , 1990, Environmental health perspectives.

[10]  E. Racker,et al.  Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ. , 1979, Biochemistry.

[11]  A. Schothorst,et al.  Photodynamic damage of the erythrocyte membrane caused by protoporphyrin in protoporphyria and in normal red blood cells. , 1972, Clinica chimica acta; international journal of clinical chemistry.

[12]  R. Langlois,et al.  BIOLOGICAL ACTIVITIES OF PHTHALOCYANINES‐IX. PHOTOSENSITIZATION OFV–79 CHINESE HAMSTER CELLS ANDEMT–6 MOUSE MAMMARY TUMOR BY SELECTIVELY SULFONATED ZINC PHTHALOCYANINES , 1988, Photochemistry and photobiology.

[13]  R. Tsien,et al.  Stimulation of the antigen receptor on WEHI-231 B lymphoma cells results in a voltage-independent increase in cytoplasmic calcium. , 1986, Journal of immunology.

[14]  T. Dubbelman,et al.  Enhancement of transbilayer mobility of a membrane lipid probe accompanies formation of membrane leaks during photodynamic treatment of erythrocytes. , 1989, Biochimica et biophysica acta.

[15]  MEMBRANE PHOTOMODIFICATION OF CARDIAC MYOCYTES: POTASSIUM AND LEAKAGE CURRENTS , 1991, Photochemistry and photobiology.

[16]  P. Braquet,et al.  Na+ and K+ transport damage induced by oxygen free radicals in human red cell membranes. , 1983, The Journal of biological chemistry.

[17]  A. Girotti.  Mechanisms of lipid peroxidation. , 1985, Journal of free radicals in biology & medicine.

[18]  H. Korn,et al.  Cyclic AMP-modulated potassium channels in murine B cells and their precursors. , 1987, Science.

[19]  R. Tsien,et al.  A new generation of Ca2+ indicators with greatly improved fluorescence properties. , 1985, The Journal of biological chemistry.

[20]  B. Trump,et al.  Calcium Regulation and Cell Injury: A Heuristic Hypothesis a , 1987 .

[21]  A. Girotti,et al.  PHOTODYNAMIC LIPID PEROXIDATION IN BIOLOGICAL SYSTEMS * , 1990, Photochemistry and photobiology.

[22]  A. Fischman,et al.  Morphologic and functional correlates of plasma membrane injury during oxidant exposure. , 1989, Free radical biology & medicine.

[23]  R. Tsien,et al.  Cytoplasmic pH and free Mg2+ in lymphocytes , 1982, The Journal of cell biology.

[24]  B. Hille.  Ionic channels of excitable membranes , 2001 .

[25]  T. Dubbelman,et al.  Photodynamic effects of hematoporphyrin-derivative on transmembrane transport systems of murine L929 fibroblasts. , 1984, Biochimica et biophysica acta.