Laser investigation of the non-uniformity of fluorescent species in dental enamel

In the present study, artificial type I and type II erosions were created on dental specimen using acetic acid and EDTA respectively. Specimens were prepared by etching extracted teeth samples in acid to varying degrees, after which the absolute fluorescence intensity ratio of the etched enamel relative to sound enamel was recorded for each specimen using 405 and 532 nm laser excitation. Results showed differences in the fluorescence ratio of etched to sound enamel for type I and II erosions. These findings suggest a non-uniform distribution of fluorescent species in the interprismatic region as compared to the prismatic region.

[1]  K. Galil,et al.  Acid etching patterns on buccal surfaces of permanent teeth. , 1979, Pediatric dentistry.

[2]  N. W. Johnson,et al.  Factors affecting the differential dissolution of human enamel in acid and EDTA. A scanning electron microscope study. , 1971, Archives of oral biology.

[3]  C. Bodecker Variations in the Lesions and Activity of Dental Caries , 1937 .

[4]  C. Dong,et al.  Multiphoton autofluorescence and second-harmonic generation imaging of the tooth. , 2007, Journal of biomedical optics.

[5]  W H Douglas,et al.  Scanning electron microscopy of type I collagen at the dentin-enamel junction of human teeth. , 1993, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[6]  Liang Zhang,et al.  Optical Measure of Enamel Health: Ability to Triage High Risk Children in Communities without Dental Practitioners , 2012, 2012 IEEE Global Humanitarian Technology Conference.

[7]  R L HARTLES,et al.  The fluorescence of teeth under ultraviolet irradiation. , 1953, The Biochemical journal.

[8]  Eric J. Seibel,et al.  Spectrally enhanced imaging of occlusal surfaces and artificial shallow enamel erosions with a scanning fiber endoscope. , 2012, Journal of biomedical optics.

[9]  S R Wood,et al.  The chemistry of enamel caries. , 2000, Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists.

[10]  Leonard Y. Nelson,et al.  Red-shifted fluorescence of sound dental hard tissue. , 2011, Journal of biomedical optics.

[11]  Diane S Lidke,et al.  Advances in high-resolution imaging – techniques for three-dimensional imaging of cellular structures , 2012, Journal of Cell Science.

[12]  M. Zellweger,et al.  Absolute autofluorescence spectra of human healthy, metaplastic, and early cancerous bronchial tissue in vivo. , 2001, Applied optics.

[13]  I. Springer,et al.  Detection of Mature Collagen in Human Dental Enamel , 2005, Calcified Tissue International.

[14]  P. Gallop,et al.  Cross-linking in collagen and elastin. , 1984, Annual review of biochemistry.

[15]  Rebecca Richards-Kortum,et al.  Realistic three-dimensional epithelial tissue phantoms for biomedical optics. , 2002, Journal of biomedical optics.

[16]  E. Rivera,et al.  Site comparisons of dentine collagen cross-links from extracted human teeth. , 1993, Archives of oral biology.

[17]  Clare M. Waterman,et al.  Advances in light-based imaging of three-dimensional cellular ultrastructure. , 2012, Current opinion in cell biology.

[18]  W. Buchalla,et al.  Comparative Fluorescence Spectroscopy Shows Differences in Noncavitated Enamel Lesions , 2005, Caries Research.

[19]  Narayanan Subhash,et al.  Clinical trial for detection of dental caries using laser-induced fluorescence ratio reference standard. , 2010, Journal of biomedical optics.

[20]  Ricardo Henriques,et al.  Single-Molecule Localization Super-Resolution Microscopy: Deeper and Faster , 2012, Microscopy and Microanalysis.