Pathogenesis of Renal Lesions in Chickens After Experimental Infection With 9a5b Newcastle Disease Virus Mutant Isolate

In this study, we investigated the pathogenesis of Newcastle disease virus (NDV) in the chicken kidney. Twenty-six 32-day-old specific pathogen-free chickens were intranasally inoculated with the 9a5b NDV mutant isolate. Kidney tissue samples, collected at 6 and 12 hours postinoculation (hpi) and 1, 2, 3, 5, and 10 days postinoculation (dpi), were analyzed by histopathology, immunohistochemistry (IHC), reverse transcription polymerase chain reaction (RT-PCR), and virus titration. Histopathologically, tubulointerstitial nephritis was detected in the renal cortex and predominantly in the medulla. Nephrotropism of 9a5b NDV was confirmed by IHC, RT-PCR, and virus isolation. Massive degenerative changes and infiltration of CD3-immunopositive cells accompanied replication of the 9a5b NDV isolate in chicken kidneys. In conclusion, pathological changes that were caused by NDV in chicken kidneys were similar to those caused by avian influenza virus, infectious bronchitis virus, and avian nephritis virus, and this highlights the importance of including NDV in the differential diagnosis of kidney disease in chickens.

[1]  T. Ito,et al.  Comparative Study on the Pathogenesis of the Generated 9a5b Newcastle Disease Virus Mutant Isolate Between Chickens and Waterfowl , 2013, Veterinary Pathology-Supplement.

[2]  Corrie Brown,et al.  Highly Divergent Virulent Isolates of Newcastle Disease Virus from the Dominican Republic Are Members of a New Genotype That May Have Evolved Unnoticed for Over 2 Decades , 2012, Journal of Clinical Microbiology.

[3]  P. Rottier,et al.  Virulence of newcastle disease virus: what is known so far? , 2011, Veterinary research.

[4]  M. Pantin-Jackwood,et al.  In vivo transcriptional cytokine responses and association with clinical and pathological outcomes in chickens infected with different Newcastle disease virus isolates using formalin-fixed paraffin-embedded samples. , 2011, Veterinary immunology and immunopathology.

[5]  Corrie Brown,et al.  Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates , 2011, Avian pathology : journal of the W.V.P.A.

[6]  P. J. Miller,et al.  Clinicopathological Characterization in Poultry of Three Strains of Newcastle Disease Virus Isolated From Recent Outbreaks , 2011, Veterinary pathology.

[7]  H. Kida,et al.  Genetic comparisons between lentogenic Newcastle disease virus isolated from waterfowl and velogenic variants , 2010, Virus Genes.

[8]  B. Seal,et al.  Pathogenesis of Newcastle Disease in Commercial and Specific Pathogen-free Turkeys Experimentally Infected with Isolates of Different Virulence , 2006, Veterinary pathology.

[9]  B. Peeters,et al.  Effect of fusion protein cleavage site mutations on virulence of Newcastle disease virus: non-virulent cleavage site mutants revert to virulence after one passage in chicken brain. , 2003, The Journal of general virology.

[10]  Y. Kawaoka,et al.  Generation of velogenic Newcastle disease viruses from a nonpathogenic waterfowl isolate by passaging in chickens. , 2002, Virology.

[11]  A. Schaeffer.  What do we know about the urinary tract infection-prone individual? , 2001, The Journal of infectious diseases.

[12]  T. Kuiken,et al.  PATHOLOGY OF NEWCASTLE DISEASE IN DOUBLE-CRESTED CORMORANTS FROM SASKATCHEWAN, WITH COMPARISON OF DIAGNOSTIC METHODS , 1999, Journal of wildlife diseases.

[13]  D. Swayne,et al.  Renal pathology in specific-pathogen-free chickens inoculated with a waterfowl-origin type A influenza virus. , 1990, Avian diseases.

[14]  H. L. Thacker,et al.  Comparison of the nephropathogenicity of four strains of infectious bronchitis virus. , 1986, Avian diseases.

[15]  T. Imada,et al.  Pathological changes in chicks inoculated with the picornavirus "avian nephritis virus". , 1979, Avian diseases.

[16]  L. Reed,et al.  A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS , 1938 .