Automated microfluidic plant chips-based plant phenotyping system

We report on the development of an enhanced and robust microfluidic plant chip-based growth and monitoring system for high-throughput phenotyping of Arabidopsis thaliana. The system consists of multiple vertical plant chips, a microfluidic hormone concentration gradient generator, multiple simple gravity pumps, and a robotic arm with a stereoscope for plant imaging. We validate this system by phenotyping the growth of Arabidopsis plants in medium containing different hormone concentrations, in a real-time manner. Our results show that Arabidopsis growth and hormone response in this system closely replicates growth on standard laboratory petri plates.

[1]  Liang Dong,et al.  Plant chip for high-throughput phenotyping of Arabidopsis. , 2014, Lab on a chip.

[2]  Ali Khademhosseini,et al.  Siphon-driven microfluidic passive pump with a yarn flow resistance controller. , 2014, Lab on a chip.

[3]  Chang Mo Hwang,et al.  Simultaneous generation of chemical concentration and mechanical shear stress gradients using microfluidic osmotic flow comparable to interstitial flow. , 2009, Lab on a chip.

[4]  J. Giovannoni,et al.  Tackling the plant proteome: practical approaches, hurdles and experimental tools. , 2004, The Plant journal : for cell and molecular biology.

[5]  Fred W. McLafferty,et al.  A New Approach for Plant Proteomics , 2003, Molecular & Cellular Proteomics.

[6]  Wolfgang Busch,et al.  A microfluidic device and computational platform for high throughput live imaging of gene expression , 2012, Nature Methods.

[7]  Eunkyoo Oh,et al.  Brassinosteroid signaling network and regulation of photomorphogenesis. , 2012, Annual review of genetics.

[8]  S. Quake,et al.  The RootChip: An Integrated Microfluidic Chip for Plant Science[W][OA] , 2011, Plant Cell.

[9]  G. Whitesides,et al.  Microfabrication meets microbiology , 2007, Nature Reviews Microbiology.

[10]  Po-Pu Liu,et al.  Large-scale screening of Arabidopsis enhancer-trap lines for seed germination-associated genes. , 2005, The Plant journal : for cell and molecular biology.

[11]  P. Zimmermann,et al.  GENEVESTIGATOR. Arabidopsis Microarray Database and Analysis Toolbox1[w] , 2004, Plant Physiology.

[12]  J. Jorgenson,et al.  Automated instrumentation for comprehensive two-dimensional high-performance liquid chromatography of proteins. , 1990, Analytical chemistry.

[13]  Nobuyuki Uozumi,et al.  Functional analysis of AtHKT1 in Arabidopsis shows that Na+ recirculation by the phloem is crucial for salt tolerance , 2003, The EMBO journal.

[14]  Sang-Hoon Lee,et al.  Gradient generation by an osmotic pump and the behavior of human mesenchymal stem cells under the fetal bovine serum concentration gradient. , 2007, Lab on a chip.

[15]  Yang Liu,et al.  Insights into the Origin and Evolution of the Plant Hormone Signaling Machinery1 , 2015, Plant Physiology.

[16]  P ? ? ? ? ? ? ? % ? ? ? ? , 1991 .

[17]  W. Marsden.  I and J , 2012 .

[18]  Andrew G. Glen,et al.  APPL , 2001 .

[20]  William H Grover,et al.  Orientation-Based Control of Microfluidics , 2016, PloS one.

[21]  Luke P. Lee,et al.  A novel high aspect ratio microfluidic design to provide a stable and uniform microenvironment for cell growth in a high throughput mammalian cell culture array. , 2005, Lab on a chip.

[22]  Nicola J. Ferrier,et al.  A high throughput robot system for machine vision based plant phenotype studies , 2012, Machine Vision and Applications.

[23]  J. Hoheisel.  Microarray technology: beyond transcript profiling and genotype analysis , 2006, Nature Reviews Microbiology.

[24]  Sang-Hoon Lee,et al.  A low-energy-consumption electroactive valveless hydrogel micropump for long-term biomedical applications. , 2011, Lab on a chip.

[25]  R. Dixon,et al.  Plant metabolomics: large-scale phytochemistry in the functional genomics era. , 2003, Phytochemistry.

[26]  Rustem F Ismagilov,et al.  Chemical stimulation of the Arabidopsis thaliana root using multi-laminar flow on a microfluidic chip. , 2010, Lab on a chip.

[27]  Qunfeng Dong,et al.  PlantGDB, plant genome database and analysis tools , 2004, Nucleic Acids Res..

[28]  M. Tester,et al.  Phenomics--technologies to relieve the phenotyping bottleneck. , 2011, Trends in plant science.

[29]  R R Mir,et al.  Array-based high-throughput DNA markers for crop improvement , 2008, Heredity.

[30]  G. Whitesides,et al.  Generation of Gradients Having Complex Shapes Using Microfluidic Networks , 2001 .

[31]  M. Packirisamy,et al.  TipChip: a modular, MEMS-based platform for experimentation and phenotyping of tip-growing cells. , 2013, The Plant journal : for cell and molecular biology.

[32]  Richard T. Lee,et al.  A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment , 2010, Biomedical microdevices.

[33]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[34]  Patrick S Schnable,et al.  Histone lysine methyltransferase SDG8 is involved in brassinosteroid-regulated gene expression in Arabidopsis thaliana. , 2014, Molecular plant.

[35]  S. Omholt,et al.  Phenomics: the next challenge , 2010, Nature Reviews Genetics.