Study of the Properties of Biotin-streptavidin Sensitive BioFETs

In this work the properties of a biotin-streptavidin BioFET have been studied numerically with homogenized boundary interface conditions as the link between the oxide of the FET and the analyte which contains the biosample. The biotin-streptavidin reaction pair is used in purification and detection of various biomolecules; the strong streptavidin-biotin bond can also be used to attach biomolecules to one another or onto a solid support. Thus this reaction pair in combination with a FET as the transducer is a powerful setup enabling the detection of a wide variety of molecules with many advantages that stem from the FET, like no labeling, no need of expensive read-out devices, the possibility to put the signal amplification and analysis on the same chip, and outdoor usage without the necessity of a lab.

[1]  Andreas Offenhäusser,et al.  Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices , 2005 .

[2]  Gerhard Klimeck,et al.  Modeling and simulation of field-effect biosensors (BioFETs) and their deployment on the nanoHUB , 2008 .

[3]  Gengfeng Zheng,et al.  Multiplexed electrical detection of cancer markers with nanowire sensor arrays , 2005, Nature Biotechnology.

[4]  M. Jamal Deen,et al.  Study of the electrolyte-insulator-semiconductor field-effect transistor (EISFET) with applications in biosensor design , 2007, Microelectron. Reliab..

[5]  M. Jamal Deen.  Highly sensitive, low-cost integrated biosensors , 2007, SBCCI '07.

[6]  M. J. Deen,et al.  Model for the field effect from layers of biological macromolecules on the gates of metal-oxide-semiconductor transistors , 2005 .

[7]  Taisun Kim,et al.  Calixarene derivative as a tool for highly sensitive detection and oriented immobilization of proteins in a microarray format through noncovalent molecular interaction , 2005, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[8]  Clemens Heitzinger,et al.  Multi - Scale Modeling and Simulation of Field-Effect Biosensors , 2008 .

[9]  S. Selberherr,et al.  Simulation of field-effect Biosensors (BioFETs) , 2008, 2008 International Conference on Simulation of Semiconductor Processes and Devices.

[10]  J. Turková,et al.  Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function. , 1999, Journal of chromatography. B, Biomedical sciences and applications.

[11]  W. Göpel,et al.  Zeta potential measurements of Ta2O5 and SiO2 thin films , 1991 .

[12]  Mark A. Reed,et al.  Label-free immunodetection with CMOS-compatible semiconducting nanowires , 2007, Nature.

[13]  Marie-Noëlle Bellon-Fontaine,et al.  Zeta Potential Measurements on Passive Metals , 1995 .

[14]  Zhiqiang Gao,et al.  Silicon nanowire arrays for label-free detection of DNA. , 2007, Analytical chemistry.

[15]  Christof M Niemeyer,et al.  Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: a comparative study. , 2004, Analytical biochemistry.

[16]  S. Selberherr.  Analysis and simulation of semiconductor devices , 1984 .

[17]  Christian A. Ringhofer,et al.  Multiscale Modeling of Planar and Nanowire Field-Effect Biosensors , 2010, SIAM J. Appl. Math..

[18]  Geunbae Lim,et al.  An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes , 2006 .

[19]  Seon-Gil Lee,et al.  BioFET sensor for detection of albumin in urine , 2008 .

[20]  P. Peluso,et al.  Optimizing antibody immobilization strategies for the construction of protein microarrays. , 2003, Analytical biochemistry.

[21]  C. Lieber,et al.  Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species , 2001, Science.

[22]  Charles M. Lieber,et al.  Direct ultrasensitive electrical detection of DNA and DNA sequence variations using nanowire nanosensors , 2004 .

[23]  Bonsang Gu,et al.  A dielectric-modulated field-effect transistor for biosensing. , 2007, Nature nanotechnology.

[24]  M. J. Deen,et al.  Noise considerations in field-effect biosensors , 2006 .

[25]  M. Harnois,et al.  Transferrin Electronic Detector for Iron Disease Diagnostics , 2006, 2006 5th IEEE Conference on Sensors.

[26]  Gerhard Klimeck,et al.  Computational aspects of the three-dimensional feature-scale simulation of silicon-nanowire field-effect sensors for DNA detection , 2007 .

[27]  P. Sorger,et al.  Electronic detection of DNA by its intrinsic molecular charge , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[28]  W. Lu,et al.  Detection of clinically relevant levels of protein analyte under physiologic buffer using planar field effect transistors. , 2008, Biosensors & bioelectronics.

[29]  J. Hoheisel,et al.  Antibody microarrays: An evaluation of production parameters , 2003, Proteomics.

[30]  Ting-Wei Tang,et al.  Discretization of flux densities in device simulations using optimum artificial diffusivity , 1995, IEEE Trans. Comput. Aided Des. Integr. Circuits Syst..

[31]  How to Make a DNA Chip , 2002 .