The activities of host and graft glial cells following retinal transplantation into the lesioned adult rat eye: developmental expression of glial markers.

We have studied the time course of the expression of glial fibrillary acidic protein (GFAP) and S-100 developmentally regulated proteins in host and graft tissue after transplantation of rat E15 retina to retinal lesion sites of adult rat hosts. Host Müller cell reactivity (GFAP staining) appeared in the peripheral retina 4.5 h after the lesion apparently in response to axotomy of the retinal ganglion cells, and then spread out in a declining wave over the whole dorsoventral extent of the retina within 1 day. From 2 days after transplantation, host glial cells appeared to migrate into the graft along the open host/graft interface, graft surfaces and blood vessels (at 8 days). Intrinsic graft glial cells (mostly Müller cells) developed approximately according to a normal time-table, but became partially reactive at 3 weeks and completely reactive at 5 weeks after transplantation. However, this reactivity seemed to have no effect on the formation of retinal laminae. Bipolar graft Müller cells were found most frequently in graft rosettes, forming an external limiting membrane around receptor inner segments, but no continuous inner limiting membrane on the vitreal surface. This was probably due to the disruption of the inner limiting membrane during transplantation, the lack or scarcity of ganglion cells or the random distribution of astrocytes in the graft.

[1]  R. Machemer,et al.  Glial cell proliferation in retinal detachment (massive periretinal proliferation). , 1975, American journal of ophthalmology.

[2]  G. Raisman,et al.  An autoradiographic study of neuronal development, vascularization and glial cell migration from hippocampal transplants labelled in intermediate explant culture , 1984, Neuroscience.

[3]  D. Anderson,et al.  Retinal detachment in the cat: the outer nuclear and outer plexiform layers. , 1983, Investigative ophthalmology & visual science.

[4]  J. Devellis,et al.  Glial-released proteins in neural intercellular communication: molecular mapping, modulation, and influence on neuronal differentiation. , 1982 .

[5]  J. R. Blair,et al.  Optimum conditions for successful transplantation of immature rat retina to the lesioned adult retina. , 1987, Brain research.

[6]  J. Wells,et al.  Xenografts of brain cells labeled in cell suspensions show growth and differentiation in septo-hippocampal transplants , 1986, Brain Research.

[7]  A. H. Bunt-Milam,et al.  Müller cell expression of glial fibrillary acidic protein after genetic and experimental photoreceptor degeneration in the rat retina. , 1984, Investigative ophthalmology & visual science.

[8]  E. Newman THE MÜLLER CELL , 1986 .

[9]  M. Cerro,et al.  Retinal transplants into the anterior chamber of the rat eye , 1987, Neuroscience.

[10]  S. Fedoroff,et al.  Development, morphology, and regional specialization of astrocytes , 1986 .

[11]  K. Weber,et al.  Monoclonal antibodies specific for glial fibrillary acidic (GFA) protein and for each of the neurofilament triplet polypeptides. , 1984, Differentiation; research in biological diversity.

[12]  Constance L. Cepko,et al.  A common progenitor for neurons and glia persists in rat retina late in development , 1987, Nature.

[13]  G. Tuccari,et al.  Distribution of glial fibrillary acidic protein in normal and gliotic human retina. , 1986, Basic and applied histochemistry.

[14]  J E Turner,et al.  Newborn rat retinal cells transplanted into a retinal lesion site in adult host eyes. , 1986, Brain research.

[15]  G. Shaw,et al.  The structure and development of the rat retina: an immunofluorescence microscopical study using antibodies specific for intermediate filament proteins. , 1983, European journal of cell biology.

[16]  T. Kuwabara,et al.  Postnatal development of the rat retina. An electron microscopic study. , 1968, Archives of ophthalmology.

[17]  J. Silver,et al.  Changing role of forebrain astrocytes during development, regenerative failure, and induced regeneration upon transplantation , 1986, The Journal of comparative neurology.

[18]  L. Eng,et al.  Glial fibrillary acidic protein in the retina of the developing albino rat: An immunoperoxidase study of paraffin‐embedded tissue , 1981, The Journal of comparative neurology.

[19]  T. Kuwabara,et al.  Development of the prenatal rat retina. , 1974, Investigative ophthalmology.

[20]  R. Adler TROPHIC INTERACTIONS IN RETINAL DEVELOPMENT AND IN RETINAL DEGENERATIONS. IN VIVO AND IN VITRO STUDIES , 1986 .

[21]  R. Lund,et al.  Influence of grafted glia cells and host mossy fibers on anomalously migrated host granule cells surviving in cortical transplants , 1982, Neuroscience.

[22]  I. Wallow,et al.  Müller's cell involvement in proliferative diabetic retinopathy. , 1987, Archives of ophthalmology.

[23]  G. Raisman,et al.  Migration of host astrocytes into superior cervical sympathetic ganglia autografted into the septal nuclei or choroid fissure of adult rats , 1986, Neuroscience.

[24]  M. Noble,et al.  Glia are a unique substrate for the in vitro growth of central nervous system neurons , 1984, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[25]  R. Noyes,et al.  Ovarian transplants to the anterior chamber of the eye. , 1958, Fertility and sterility.

[26]  A. Bignami,et al.  The radial glia of Müller in the rat retina and their response to injury. An immunofluorescence study with antibodies to the glial fibrillary acidic (GFA) protein. , 1979, Experimental eye research.

[27]  Roy Pe,et al.  Retinal transplantation from fetal to maternal mammalian eye. , 1959 .

[28]  J. Stone,et al.  Relationship between astrocytes, ganglion cells and vasculature of the retina , 1987, The Journal of comparative neurology.

[29]  M. Tso,et al.  Massive retinal gliosis. A reactive proliferation of Müller cells. , 1986, Archives of ophthalmology.

[30]  R. W. Young Cell differentiation in the retina of the mouse , 1985, The Anatomical record.

[31]  G. K. Smelser,et al.  Electron microscopic study of the development of retinal Müllerian cells. , 1973, Investigative ophthalmology.

[32]  R. Aramant,et al.  Donor age influences on the success of retinal grafts to adult rat retina. , 1988, Investigative ophthalmology & visual science.

[33]  Don H. Anderson,et al.  Glial fibrillary acidic protein increases in Müller cells after retinal detachment. , 1987, Experimental eye research.

[34]  J. Sheffield,et al.  Retinal flat cells are a substrate that facilitates retinal neuron growth and fiber formation. , 1986, Investigative ophthalmology & visual science.

[35]  G. Raisman,et al.  Membrane specializations and extracellular material associated with host astrocytes in peripheral neural transplants , 1987, Neuroscience.

[36]  F. Michetti,et al.  Localization of S-100 protein in Müller cells of the retina--2. Electron microscopical immunocytochemistry. , 1983, Investigative ophthalmology & visual science.

[37]  P. Bovolenta,et al.  Glial filament protein expression in astroglia in the mouse visual pathway. , 1987, Brain research.

[38]  F. Roberge,et al.  Chemotaxis of rat retinal glia to growth factors found in repairing wounds. , 1987, Investigative ophthalmology & visual science.

[39]  A. Bignami,et al.  Immunohistochemical demonstration of glial fibrillary acidic protein in normal rat Müller glia and retinal astrocytes , 1985, Neuroscience Letters.