Multiple release kinetics of targeted drug from gold nanorod embedded polyelectrolyte conjugates induced by near-infrared laser irradiation.

The conjugates of gold nanorods and the model drug, fluorescein isothiocyanate (FITC), embedded inside polyelectrolytes (GNRs/FITC@PLE) were synthesized to study the release kinetics of FITC under femtosecond near-infrared (NIR) laser irradiation. The optical and structural properties of GNRs/FITC@PLE conjugates before and after laser treatments were examined using UV-vis spectroscopy, confocal microscopy, and transmission electron microscopy (TEM). The release of FITC from the conjugates was induced by the heat generated from gold nanorods under laser irradiation. The concentration of released FITC was measured as the time of continuous and periodic laser irradiation was varied. Within 5 min of the laser exposure, the release rates of FITC exhibited zero-order and first-order kinetics under continuous and periodic irradiation, respectively. Furthermore, a drug release system was designed based on the conjugates of gold nanorods and the anticancer drug, paclitaxel (PTX), embedded inside polyelectrolytes (GNRs/PTX@PLE). The conjugates were applied for in vitro studies with breast cancer cells. The release of PTX from the conjugates was triggered by NIR laser irradiation, and the inhibition rates of the cells showed strong dependencies on the irradiation modes and time. The results suggested that the multiple releases of PTX from the conjugates can be controlled by laser irradiation within a long period of time. Our system holds great potential for future therapeutic applications on breast cancers.

[1]  M. Brewster,et al.  Novel self-assembling PEG-p-(CL-co-TMC) polymeric micelles as safe and effective delivery system for paclitaxel. , 2009, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[2]  N. Nakashima,et al.  Photothermal reshaping of gold nanorods depends on the passivating layers of the nanorod surfaces. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[3]  J. Remington,et al.  Antibody responses to toxoplasma antigens in mice infected with strains of different virulence , 1980, Infection and immunity.

[4]  Kyujung Kim,et al.  Multifunctional nanoparticles for photothermally controlled drug delivery and magnetic resonance imaging enhancement. , 2008, Small.

[5]  Baowei Fei,et al.  Highly efficient drug delivery with gold nanoparticle vectors for in vivo photodynamic therapy of cancer. , 2008, Journal of the American Chemical Society.

[6]  B. Nikoobakht,et al.  種結晶を媒介とした成長法を用いた金ナノロッド(NR)の調製と成長メカニズム , 2003 .

[7]  서진석,et al.  Multifunctional nanoparticles for photothermally controlled drug delivery and magnetic resonance imaging enhancement , 2008 .

[8]  Ming Lei,et al.  Hard and soft micromachining for BioMEMS: review of techniques and examples of applications in microfluidics and drug delivery. , 2004, Advanced drug delivery reviews.

[9]  H. Dai,et al.  Soluble single-walled carbon nanotubes as longboat delivery systems for platinum(IV) anticancer drug design. , 2007, Journal of the American Chemical Society.

[10]  E. Fabrizio,et al.  Porous silicon as drug carrier for controlled delivery of doxorubicin anticancer agent , 2006 .

[11]  R. Misra,et al.  On the suitability of nanocrystalline ferrites as a magnetic carrier for drug delivery: functionalization, conjugation and drug release kinetics. , 2007, Acta biomaterialia.

[12]  F. Xiao,et al.  pH-responsive carrier system based on carboxylic acid modified mesoporous silica and polyelectrolyte for drug delivery , 2005 .

[13]  Xiaohu Gao,et al.  Spectrally tunable leakage-free gold nanocontainers. , 2009, Journal of the American Chemical Society.

[14]  C. Murphy,et al.  Polyelectrolyte-Coated Gold Nanorods: Synthesis, Characterization and Immobilization , 2005 .

[15]  M. El-Sayed,et al.  Laser-Induced Shape Changes of Colloidal Gold Nanorods Using Femtosecond and Nanosecond Laser Pulses , 2000 .

[16]  H. Dai,et al.  Carbon nanotubes as intracellular protein transporters: generality and biological functionality. , 2005, Journal of the American Chemical Society.

[17]  J C Shah,et al.  Biodegradable polyanhydride devices of cefazolin sodium, bupivacaine, and taxol for local drug delivery: preparation, and kinetics and mechanism of in vitro release. , 1998, Journal of controlled release : official journal of the Controlled Release Society.

[18]  Yi-Cheng Chen,et al.  DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation. , 2006, Journal of the American Chemical Society.

[19]  D. Balding,et al.  HLA Sequence Polymorphism and the Origin of Humans , 2006 .

[20]  Helmuth Möhwald,et al.  Near-IR remote release from assemblies of liposomes and nanoparticles. , 2009, Angewandte Chemie.

[21]  K. Soppimath,et al.  pH‐Triggered Thermally Responsive Polymer Core–Shell Nanoparticles for Drug Delivery , 2005 .

[22]  Ying-Jie Zhu,et al.  Surfactant-Free Preparation and Drug Release Property of Magnetic Hollow Core/Shell Hierarchical Nanostructures , 2008 .

[23]  D. M. Lynn,et al.  Tunable drug release from hydrolytically degradable layer-by-layer thin films. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[24]  Itaru Honma,et al.  Ultrasound‐Triggered Smart Drug Release from a Poly(dimethylsiloxane)– Mesoporous Silica Composite , 2006 .

[25]  K. Hamad-Schifferli,et al.  Effect of ligands on thermal dissipation from gold nanorods. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[26]  K. Hamad-Schifferli,et al.  Selective release of multiple DNA oligonucleotides from gold nanorods. , 2009, ACS nano.

[27]  Christine Jérôme,et al.  Paclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation. , 2009, Journal of controlled release : official journal of the Controlled Release Society.

[28]  N. Kotov,et al.  Gold nanoparticles enhance the anti-leukemia action of a 6-mercaptopurine chemotherapeutic agent. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[29]  Matthias Karg,et al.  Nanorod-coated PNIPAM microgels: thermoresponsive optical properties. , 2007, Small.

[30]  Jianlin Shi,et al.  Uniform Rattle‐type Hollow Magnetic Mesoporous Spheres as Drug Delivery Carriers and their Sustained‐Release Property , 2008 .

[31]  H. Dai,et al.  Nanotube molecular transporters: internalization of carbon nanotube-protein conjugates into Mammalian cells. , 2004, Journal of the American Chemical Society.

[32]  Shanshan Huang,et al.  Synthesis and Characterization of Magnetic FexOy@SBA-15 Composites with Different Morphologies for Controlled Drug Release and Targeting , 2008 .

[33]  M. El-Sayed,et al.  Spectral Properties and Relaxation Dynamics of Surface Plasmon Electronic Oscillations in Gold and Silver Nanodots and Nanorods , 1999 .

[34]  P. Cullis,et al.  Drug Delivery Systems: Entering the Mainstream , 2004, Science.

[35]  W. Saltzman,et al.  Drug delivery: Stealth particles give mucus the slip. , 2009, Nature materials.

[36]  T. Niidome,et al.  Stable incorporation of gold nanorods into N-isopropylacrylamide hydrogels and their rapid shrinkage induced by near-infrared laser irradiation. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[37]  K. O’Grady,et al.  柔軟記録媒体のための金属粒子(MP)技術の開発 , 2008 .

[38]  Y. Bae,et al.  Drug release from biodegradable injectable thermosensitive hydrogel of PEG-PLGA-PEG triblock copolymers. , 2000, Journal of controlled release : official journal of the Controlled Release Society.

[39]  Yi Yan Yang,et al.  The co-delivery of paclitaxel and Herceptin using cationic micellar nanoparticles. , 2009, Biomaterials.

[40]  R. K. Harrison,et al.  Thermal analysis of gold nanorods heated with femtosecond laser pulses , 2008, Journal of physics D: Applied physics.

[41]  J. Vadgama,et al.  Taxol induced apoptosis regulates amino acid transport in breast cancer cells , 2007, Apoptosis.

[42]  Vincent Noireaux,et al.  In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles , 2002, Science.

[43]  Stanislaus S. Wong,et al.  Functionalized single-walled carbon nanotubes as rationally designed vehicles for tumor-targeted drug delivery. , 2008, Journal of the American Chemical Society.

[44]  Timo Laaksonen,et al.  Gold nanoparticles enable selective light-induced contents release from liposomes. , 2007, Journal of controlled release : official journal of the Controlled Release Society.

[45]  Kenji Kaneko,et al.  Modification of gold nanorods using phosphatidylcholine to reduce cytotoxicity. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[46]  S. Nie,et al.  Quantum dot bioconjugates for ultrasensitive nonisotopic detection. , 1998, Science.

[47]  M. El-Sayed,et al.  Laser photothermal melting and fragmentation of gold nanorods: Energy and laser pulse-width dependence , 1999 .

[48]  Wenbin Lin,et al.  Nanoscale coordination polymers for platinum-based anticancer drug delivery. , 2008, Journal of the American Chemical Society.

[49]  H. Dai,et al.  Noncovalent functionalization of carbon nanotubes by fluorescein-polyethylene glycol: supramolecular conjugates with pH-dependent absorbance and fluorescence. , 2007, Journal of the American Chemical Society.

[50]  Shiladitya Sengupta,et al.  Temporal targeting of tumour cells and neovasculature with a nanoscale delivery system , 2005, Nature.

[51]  Kristi S Anseth,et al.  Controlling Affinity Binding with Peptide‐Functionalized Poly(ethylene glycol) Hydrogels , 2009, Advanced functional materials.

[52]  Gérard Férey,et al.  Flexible porous metal-organic frameworks for a controlled drug delivery. , 2008, Journal of the American Chemical Society.

[53]  Dean-Mo Liu,et al.  Magnetic-sensitive silica nanospheres for controlled drug release. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[54]  V. Colvin.  The potential environmental impact of engineered nanomaterials , 2003, Nature Biotechnology.

[55]  Anand Gole,et al.  Targeted photothermal lysis of the pathogenic bacteria, Pseudomonas aeruginosa, with gold nanorods. , 2008, Nano letters.

[56]  Kyeongjae Cho,et al.  Thermal Expansion and Diffusion Coefficients of Carbon Nanotube-Polymer Composites , 2002, cond-mat/0203349.

[57]  Younan Xia,et al.  Gold nanocages covered by smart polymers for controlled release with near-infrared light , 2009, Nature materials.

[58]  D. Reinhardt,et al.  Modification of the Structure and Function of Fibrillin-1 by Homocysteine Suggests a Potential Pathogenetic Mechanism in Homocystinuria* , 2005, Journal of Biological Chemistry.

[59]  J. Xue,et al.  Targeted and intracellular delivery of paclitaxel using multi-functional polymeric micelles. , 2007, Biomaterials.

[60]  Jung Ho Yu,et al.  Designed Fabrication of a Multifunctional Polymer Nanomedical Platform for Simultaneous Cancer‐ Targeted Imaging and Magnetically Guided Drug Delivery , 2008 .

[61]  Y. Rao,et al.  Polymer Nanocomposites with a Low Thermal Expansion Coefficient , 2008 .

[62]  S. Caruthers,et al.  Ligand-directed nanobialys as theranostic agent for drug delivery and manganese-based magnetic resonance imaging of vascular targets. , 2008, Journal of the American Chemical Society.

[63]  Robert Langer,et al.  Small-scale systems for in vivo drug delivery , 2003, Nature Biotechnology.

[64]  K. Hamad-Schifferli,et al.  Release Mechanism of Octadecyl Rhodamine B Chloride from Au Nanorods by Ultrafast Laser Pulses , 2009 .

[65]  Hong Ding,et al.  Gold Nanorods Coated with Multilayer Polyelectrolyte as Contrast Agents for Multimodal Imaging , 2007 .

[66]  Timothy S. Troutman,et al.  Light‐Induced Content Release from Plasmon‐Resonant Liposomes , 2009, Advanced materials.

[67]  W. Semmler,et al.  Receptor-targeted optical imaging of tumors with near-infrared fluorescent ligands , 2001, Nature Biotechnology.

[68]  Monty Liong,et al.  Multifunctional inorganic nanoparticles for imaging, targeting, and drug delivery. , 2008, ACS nano.

[69]  James B. Mitchell,et al.  Taxol in combination with doxorubicin or etoposide possible antagonism in vitro , 1993, Cancer.

[70]  R. Weissleder.  A clearer vision for in vivo imaging , 2001, Nature Biotechnology.

[71]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[72]  N. Jana,et al.  Preparation of Polystyrene- and Silica-Coated Gold Nanorods and Their Use as Templates for the Synthesis of Hollow Nanotubes , 2001 .