Lack of association between endothelial nitric oxide synthase polymorphisms and Henoch-Schönlein purpura.

OBJECTIVE To assess the influence of endothelial nitric oxide synthase (eNOS) polymorphisms in the susceptibility and clinical expression of patients with cutaneous vasculitis fulfilling classification criteria for Henoch-Schönlein purpura (HSP). METHODS Fifty patients from Northwest Spain with primary cutaneous vasculitis classified as HSP were studied. Patients and ethnically matched controls (n = 117) were genotyped by polymerase chain reaction techniques for a variable-number tandem-repeat polymorphism in intron 4, a T/C polymorphism at position -786 in the promoter region, and a polymorphism in exon 7 (298Glu/Asp or 5557G/T) of the eNOS gene. RESULTS No differences in allele or genotype frequencies for any of the individual eNOS polymorphisms were observed between patients fulfilling HSP classification criteria and controls, or when patients were stratified for the presence of nephritis or joint or gastrointestinal manifestations. In the HSP group no linkage disequilibrium between these polymorphisms was found. No significant difference in haplotype frequencies was observed between patients and controls. CONCLUSION Our results do not support a role for these polymorphisms in the susceptibility to HSP.

[1]  W. Ollier,et al.  Endothelial nitric oxide synthase haplotype associations in biopsy-proven giant cell arteritis. , 2003, The Journal of rheumatology.

[2]  J. Townend,et al.  Diffuse endothelial dysfunction is common to ANCA associated systemic vasculitis and polyarteritis nodosa , 2003, Annals of the rheumatic diseases.

[3]  W. Thomson,et al.  Interleukin 8 gene polymorphism is associated with increased risk of nephritis in cutaneous vasculitis. , 2002, The Journal of rheumatology.

[4]  W. Thomson,et al.  Interleukin 1 receptor antagonist gene polymorphism is associated with severe renal involvement and renal sequelae in Henoch-Schönlein purpura. , 2002, The Journal of rheumatology.

[5]  R. Abbate,et al.  High prevalence of polymorphisms of angiotensin-converting enzyme (I/D) and endothelial nitric oxide synthase (Glu298Asp) in patients with systemic sclerosis. , 2002, The American journal of medicine.

[6]  W. Thomson,et al.  HLA-B35 association with nephritis in Henoch-Schönlein purpura. , 2002, The Journal of rheumatology.

[7]  I. Olivieri,et al.  Endothelial nitric oxide synthase gene polymorphisms in Behçet's disease. , 2002, The Journal of rheumatology.

[8]  W. Thomson,et al.  HLA-DRB1*01 association with Henoch-Schönlein purpura in patients from northwest Spain. , 2001, The Journal of rheumatology.

[9]  W. Thomson,et al.  Polymorphism at codon 469 of the intercellular adhesion molecule-1 locus is associated with protection against severe gastrointestinal complications in Henoch-Schönlein purpura. , 2001, The Journal of rheumatology.

[10]  J. Townend,et al.  Endothelial Function : Lessons for Atherosclerotic Disease ? Suppression of Inflammation in Primary Systemic Vasculitis Restores Vascular , 2000 .

[11]  K. Nakao,et al.  T-786-->C mutation in the 5'-flanking region of the endothelial nitric oxide synthase gene is associated with coronary spasm. , 1999, Circulation.

[12]  H. Markus,et al.  Endothelial nitric oxide synthase exon 7 polymorphism, ischemic cerebrovascular disease, and carotid atheroma. , 1998, Stroke.

[13]  S. Moncada.  Nitric Oxide in the Vasculature: Physiology and Pathophysiology , 1997, Annals of the New York Academy of Sciences.

[14]  G. Hunder,et al.  Hypersensitivity vasculitis and Henoch-Schönlein purpura: a comparison between the 2 disorders. , 1992, The Journal of rheumatology.