Organochlorine pesticide levels in Clarias gariepinus from polluted freshwater impoundments in South Africa and associated human health risks.

There are increasing concerns regarding the safe human consumption of fish from polluted, freshwater impoundments. The aim of this study was to analyse the muscle tissue of the sharptooth catfish Clarias gariepinus for selected organo-chlorine pesticides (OCPs) and to perform a human health risk assessment using a standard protocol described by the United States Environmental Protection Agency (US EPA). Fish were collected from the polluted Roodeplaat-(RDPD), Rietvlei-(RVD) and Hartbeespoort (HBPD) Dam impoundments located in the north-eastern regions of South Africa. GC-MS analyses showed levels of various OCPs in fish muscle samples from all three impoundments. For fish collected from the RDPD, p,p'-DDE, endosulfan, lindane and β- and δ-HCH were the most prevalent OCPs detected, while p,p'-DDE and endosulfan were the most predominant in fish from the RVD. Lindane and β- and δ-HCH were the main OCPs detected in fish from the HBPD. Dieldrin was the only OCP detected at concentrations for which a cancer risk and a hazard index above the acceptable risk levels were estimated. This was the case for fish from both the RDPD and RVD impoundments. No toxic risk was estimated should fish from the HBPD be consumed.

[1]  Eiji Katsura,et al.  Screening for estrogen and androgen receptor activities in 200 pesticides by in vitro reporter gene assays using Chinese hamster ovary cells. , 2004, Environmental health perspectives.

[2]  Ord,et al.  Exposure Factors Handbook (2011 Edition) , 2015 .

[3]  A. Polder,et al.  Organochlorine contaminants in cormorant, darter, egret, and ibis eggs from South Africa. , 2008, Chemosphere.

[4]  B. Genthe,et al.  Methodology for the assessment of human health risks associated with the consumption of chemical contaminated freshwater fish in South Africa , 2004 .

[5]  J. Giesy,et al.  Human health risk assessment of organochlorines associated with fish consumption in a coastal city in China. , 2005, Environmental pollution.

[6]  J. Konz,et al.  Exposure factors handbook , 1989 .

[7]  Godfred Darko,et al.  Persistent organochlorine pesticide residues in fish, sediments and water from Lake Bosomtwi, Ghana. , 2008, Chemosphere.

[8]  J. van Vuren,et al.  Histological evidence of intersex in feral sharptooth catfish (Clarias gariepinus) from an estrogen‐polluted water source in Gauteng, South Africa , 2004, Environmental toxicology.

[9]  M. Storelli.  Potential human health risks from metals (Hg, Cd, and Pb) and polychlorinated biphenyls (PCBs) via seafood consumption: estimation of target hazard quotients (THQs) and toxic equivalents (TEQs). , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[10]  C. Propper,et al.  Endosulfan exposure disrupts pheromonal systems in the red-spotted newt: a mechanism for subtle effects of environmental chemicals. , 2001, Environmental health perspectives.

[11]  L. Gray,et al.  Persistent DDT metabolite p,p'–DDE is a potent androgen receptor antagonist , 1995, Nature.

[12]  N. Pagès,et al.  [Cardiotoxicity of lindane, a gamma isomer of hexachlorocyclohexane]. , 2002, Journal de la Societe de biologie.

[13]  B. Graubard,et al.  Polychlorinated biphenyls and risk of testicular germ cell tumors. , 2009, Cancer research.

[14]  M. Wolff,et al.  DDT and Breast Cancer in Young Women: New Data on the Significance of Age at Exposure , 2007, Environmental health perspectives.

[15]  J. Jorgenson,et al.  Aldrin and dieldrin: a review of research on their production, environmental deposition and fate, bioaccumulation, toxicology, and epidemiology in the United States. , 2001, Environmental health perspectives.

[16]  J. Frémy,et al.  Interlaboratory study of a multiresidue gas chromatographic method for determination of organochlorine and pyrethroid pesticides and polychlorobiphenyls in milk, fish, eggs, and beef fat. , 2002, Journal of AOAC International.

[17]  S. K. Nag,et al.  Persistent organochlorine pesticide residues in animal feed , 2011, Environmental monitoring and assessment.

[18]  J. Giesy,et al.  Specific binding of hydroxylated polychlorinated biphenyl metabolites and other substances to bovine calf uterine estrogen receptor: structure-binding relationships. , 1999, The Science of the total environment.

[19]  A. Christoforidis,et al.  Organochlorine and mercury contamination in fish tissues from the River Nestos, Greece. , 2008, Chemosphere.

[20]  John P. Sumpter,et al.  Widespread Sexual Disruption in Wild Fish , 1998 .

[21]  M. Longnecker,et al.  Associations between Plasma DDE Levels and Immunologic Measures in African-American Farmers in North Carolina , 2004, Environmental health perspectives.

[22]  J. van Dyk,et al.  Histopathological changes in two potential indicator fish species from a hyper-eutrophic freshwater ecosystem in South Africa: a baseline study , 2012 .

[23]  J. van Dyk,et al.  Intersex in feral indigenous freshwater Oreochromis mossambicus, from various parts in the Luvuvhu River, Limpopo Province, South Africa. , 2010, Ecotoxicology and environmental safety.

[24]  L. Hagmar,et al.  Exposure to persistent organochlorine pollutants and type 2 diabetes mellitus , 2007, Human & experimental toxicology.

[25]  I. Barnhoorn,et al.  Histological alterations in the testes and ovaries of the sharptooth catfish (Clarias gariepinus) from an urban nature reserve in South Africa , 2010 .

[26]  M. Mccarthy.  DDT and breast cancer , 1993, The Lancet.

[27]  H. Bouwman.  South Africa and the Stockholm Convention on Persistent Organic Pollutants : science policy , 2004 .

[28]  O. Receveur,et al.  Consumption of freshwater fish in Kahnawake: risks and benefits. , 1999, Environmental research.

[29]  P. Cullinan,et al.  Prenatal Dichlorodiphenyldichloroethylene (DDE) and Asthma in Children , 2005, Environmental health perspectives.

[30]  I. Barnhoorn,et al.  Sperm motility and testicular histology as reproductive indicators of fish health of two feral fish species from a currently DDT sprayed area, South Africa , 2010 .

[31]  C. V. Ginkel.  Eutrophication: Present reality and future challenges for South Africa , 2011 .

[32]  B. Graubard,et al.  Persistent organochlorine pesticides and risk of testicular germ cell tumors. , 2008, Journal of the National Cancer Institute.

[33]  A. Aktumsek,et al.  Some organochlorine pesticide residues in fish species in Konya, Turkey. , 2009, Chemosphere.

[34]  J. Sunyer,et al.  Effects of PCBs, p,p′-DDT, p,p′-DDE, HCB and β-HCH on thyroid function in preschool children , 2007, Occupational and Environmental Medicine.

[35]  H. Bouwman,et al.  DDT residues in water, sediment, domestic and indigenous biota from a currently DDT-sprayed area. , 2009, Chemosphere.

[36]  J. Szlinder-Richert,et al.  Organochlorine pesticides in fish from the southern Baltic Sea: levels, bioaccumulation features and temporal trends during the 1995-2006 period. , 2008, Marine pollution bulletin.

[37]  S. Hill,et al.  Identification of environmental chemicals with estrogenic activity using a combination of in vitro assays. , 1996, Environmental health perspectives.

[38]  J. Giesy,et al.  Risk assessment for human consumption of perfluorinated compound-contaminated freshwater and marine fish from Hong Kong and Xiamen. , 2011, Chemosphere.

[39]  K. Narayan,et al.  Prevalence of Self-Reported Diabetes and Exposure to Organochlorine Pesticides among Mexican Americans: Hispanic Health and Nutrition Examination Survey, 1982–1984 , 2007, Environmental health perspectives.

[40]  N. Jewell,et al.  In Utero Exposure to Dichlorodiphenyltrichloroethane (DDT) and Dichlorodiphenyldichloroethylene (DDE) and Neurodevelopment Among Young Mexican American Children , 2006, Pediatrics.

[41]  Yingxin Yu,et al.  Organochlorine pesticides in fish from Taihu Lake, China, and associated human health risk assessment. , 2013, Ecotoxicology and environmental safety.