Characterization of an autostimulatory substance produced by Escherichia coli.

The recovery of dilute populations of stationary phase cells of Escherichia coli was studied using an automatic growth analyser. The addition of 30% supernatant from 2-d-old stationary phase cells of the organism reproducibly shortened the apparent lag times by 22-57.5%, depending on the age of the inoculum. True lag times, as determined by colony counts, of stationary phase cells were reduced by supernatant addition by 41-62%. The growth-stimulating substance was characterized and partly purified from supernatants: the active material was shown to be dialysable, heat-stable, acid- and alkali-stable and protease-resistant. Extraction with ethyl acetate or ion-exchange resins was not successful, but the active material could be quantitatively extracted with ethanol after saturation with salt. It is concluded that the active substance is a small, non-proteinaceous, non-ionic organic molecule. Separation of extracts by HPLC indicated that the stimulatory substance is weakly hydrophobic and has retention times similar to those of uracil. So far, however, the exact chemical identity of the active substance has not been elucidated.

[1]  P. Barrow,et al.  Growth suppression in early-stationary-phase nutrient broth cultures of Salmonella typhimurium and Escherichia coli is genus specific and not regulated by sigma S , 1996, Journal of bacteriology.

[2]  W. Hull,et al.  Structure elucidation and chemical synthesis of stigmolone, a novel type of prokaryotic pheromone. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[3]  Ajl Sj,et al.  Replacement of CO2 in heterotrophic metabolism. , 1948 .

[4]  M. Lyte,et al.  Production of an autoinducer of growth by norepinephrine cultured Escherichia coli O157:H7. , 1996, FEMS microbiology letters.

[5]  P. Loewen.  Levels of glutathione in Escherichia coli. , 1979, Canadian journal of biochemistry.

[6]  P. Rather,et al.  Escherichia coli genes regulated by cell-to-cell signaling. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[7]  S. Swift,et al.  Gram-negative bacterial communication by N-acyl homoserine lactones: a universal language? , 1994, Trends in microbiology.

[8]  D B Kell,et al.  Bacterial dormancy and culturability: the role of autocrine growth factors. , 2000, Current opinion in microbiology.

[9]  A. Grafen,et al.  Pheromones, social behaviour and the functions of secondary metabolism in bacteria. , 1995, Trends in ecology & evolution.

[10]  S. Rice,et al.  Quorum‐sensing cross talk: isolation and chemical characterization of cyclic dipeptides from Pseudomonas aeruginosa and other Gram‐negative bacteria , 1999, Molecular microbiology.

[11]  T. Baldwin,et al.  Control of cell division in Escherichia coli: regulation of transcription of ftsQA involves both rpoS and SdiA-mediated autoinduction. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[12]  D. Kell,et al.  Dormancy in Stationary-Phase Cultures of Micrococcus luteus: Flow Cytometric Analysis of Starvation and Resuscitation , 1993, Applied and environmental microbiology.

[13]  E. Greenberg,et al.  Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Hinton,et al.  The Escherichia coli genome sequence: the end of an era or the start of the FUN? , 1997, Molecular microbiology.

[15]  D B Kell,et al.  The inhibition by CO2 of the growth and metabolism of micro-organisms. , 1989, The Journal of applied bacteriology.

[16]  J. Shapiro.  Thinking about bacterial populations as multicellular organisms. , 1998, Annual review of microbiology.

[17]  D. Kell,et al.  Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. , 1996, Microbiological reviews.

[18]  E. Greenberg,et al.  Cell-to-cell signaling in the symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum: autoinduction of a stationary phase and rhizosphere-expressed genes , 1996, Journal of bacteriology.

[19]  M. Surette,et al.  Quorum sensing in Escherichia coli and Salmonella typhimurium. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[20]  S. Kjelleberg,et al.  Extracellular Signal Molecule(s) Involved in the Carbon Starvation Response of Marine Vibrio sp. Strain S14 , 1998, Journal of bacteriology.

[21]  G. Salmond,et al.  The bacterial ‘enigma’: cracking the code of cell–cell communication , 1995, Molecular microbiology.

[22]  M. Raff,et al.  Social controls on cell survival and cell death , 1992, Nature.

[23]  R. Kolter,et al.  An E. coli promoter induced by the cessation of growth , 1987, Molecular microbiology.

[24]  M. Hsieh,et al.  Growth-phase-dependent transcriptional regulation of the pcm and surE genes required for stationary-phase survival of Escherichia coli. , 1997, Microbiology.

[25]  K. Nordström,et al.  Quorum-sensing acts at initiation of chromosomal replication in Escherichia coli. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[26]  D B Kell,et al.  A bacterial cytokine. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[27]  E. Greenberg,et al.  Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators. , 1996, Annual review of microbiology.

[28]  C. Hinshelwood,et al.  51. Physicochemical aspects of bacterial growth. Part IX. The lag phase of Bact. lactis aerogenes , 1943 .

[29]  G. Salmond,et al.  Quorum sensing: a population-density component in the determination of bacterial phenotype. , 1996, Trends in biochemical sciences.

[30]  M R Barer,et al.  Bacterial viability and culturability. , 1999, Advances in microbial physiology.

[31]  N. W. Davis,et al.  The complete genome sequence of Escherichia coli K-12. , 1997, Science.

[32]  U. Rinas,et al.  Entry of Escherichia coli into stationary phase is indicated by endogenous and exogenous accumulation of nucleobases , 1995, Applied and environmental microbiology.

[33]  D. Kell,et al.  Do bacteria need to communicate with each other for growth? , 1996, Trends in microbiology.

[34]  P. Hartman,et al.  Export of glutathione by some widely used Salmonella typhimurium and Escherichia coli strains , 1986, Journal of bacteriology.

[35]  J. García‐Lara,et al.  An extracellular factor regulates expression of sdiA, a transcriptional activator of cell division genes in Escherichia coli , 1996, Journal of bacteriology.

[36]  D. Wheatley,et al.  Signaling in unicellular eukaryotes. , 1998, International review of cytology.

[37]  D. Kell,et al.  Estimation of dormant Micrococcus luteus cells by penicillin lysis and by resuscitation in cell-free spent culture medium at high dilution , 1994 .

[38]  D. Kaiser,et al.  How and why bacteria talk to each other , 1993, Cell.

[39]  F. Neidhardt,et al.  Culture Medium for Enterobacteria , 1974, Journal of bacteriology.

[40]  M. Surette,et al.  Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[41]  P. Freestone,et al.  The Mammalian Neuroendocrine Hormone Norepinephrine Supplies Iron for Bacterial Growth in the Presence of Transferrin or Lactoferrin , 2000, Journal of bacteriology.

[42]  E. Greenberg,et al.  Self perception in bacteria: quorum sensing with acylated homoserine lactones. , 1998, Current opinion in microbiology.