Application of Functional Genomics and Proteomics to Plant Cryopreservation

Plant cryobiology has primarily emerged from the classical fields of cryobiology and plant stress physiology. Cryopreservation tools are now available to geneticists for germplasm preservation and the field itself is advancing significantly through the use of molecular techniques. Long-term preservation of vegetatively propagated tissues can minimize the risks of long-term maintenance under tissue culture or field conditions. Cells can be successfully cryopreserved when the adverse affects of ice crystal formation are mitigated by the removal of water or procedures to limit ice formation and crystal growth. The addition of cryoprotectant solutions to hydrated cells may improve the survival of microdissected shoot tips or embryonic axes. Recent discoveries in the genetic pathways leading to cold acclimation and freezing tolerance suggest the involvement of key cold-regulated genes in the acquisition of cold tolerance in plant tissues. Model systems of banana and Arabidopsis have revealed the involvement of genes and proteins in the glycolytic and other metabolic pathways, particularly processes involved in dehydration tolerance, osmoprotection, and membrane transport. Furthermore, successful recovery appears to be dependent upon the presence of antioxidant protection from reactive oxygen species. Characterization of specific genes and proteins will lead to significant advances in plant cryobiology research.

[1]  E. Benson,et al.  Cryopreservation induces temporal DNA methylation epigenetic changes and differential transcriptional activity in Ribes germplasm. , 2009, Plant physiology and biochemistry : PPB.

[2]  K. Yamaguchi-Shinozaki,et al.  Transcriptional Regulatory Networks in Response to Abiotic Stresses in Arabidopsis and Grasses1 , 2009, Plant Physiology.

[3]  B. Reed,et al.  Genetic and epigenetic stability of cryopreserved and cold-stored hops (Humulus lupulus L.). , 2008, Cryobiology.

[4]  D. Hincha.  Effects of α‐tocopherol (vitamin E) on the stability and lipid dynamics of model membranes mimicking the lipid composition of plant chloroplast membranes , 2008, FEBS letters.

[5]  L. Zolla,et al.  Proteomics applied on plant abiotic stresses: role of heat shock proteins (HSP). , 2008, Journal of proteomics.

[6]  Kazuki Saito,et al.  Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells. , 2008, Cryo letters.

[7]  M. Thorne,et al.  Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. , 2008, Journal of insect physiology.

[8]  D. Burritt.  Efficient cryopreservation of adventitious shoots of Begonia x erythrophylla using encapsulation–dehydration requires pretreatment with both ABA and proline , 2008, Plant Cell, Tissue and Organ Culture.

[9]  B. Devreese,et al.  Proteome analysis of non-model plants: a challenging but powerful approach. , 2008, Mass spectrometry reviews.

[10]  E. Benson.  Cryopreservation of Phytodiversity: A Critical Appraisal of Theory & Practice , 2008 .

[11]  M. Clark,et al.  How insects survive the cold: molecular mechanisms—a review , 2008, Journal of Comparative Physiology B.

[12]  H. Matsumura,et al.  Functional genomics in a non-model crop: transcriptomics or proteomics? , 2008, Physiologia plantarum.

[13]  M. E. Dulloo,et al.  Assessment of genetic and epigenetic changes following cryopreservation in papaya , 2008, Plant Cell Reports.

[14]  C. Walters,et al.  Structural Dynamics and Desiccation Damage in Plant Reproductive Organs , 2008 .

[15]  C. Richards,et al.  Genebanks in the post-genomic age: Emerging roles and anticipated uses , 2008 .

[16]  D. Hincha,et al.  LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana , 2008, BMC Genomics.

[17]  R. Reinhardt,et al.  Surviving extreme polar winters by desiccation: clues from Arctic springtail (Onychiurus arcticus) EST libraries , 2007, BMC Genomics.

[18]  G. Volk,et al.  Plasmolysis and recovery of different cell types in cryoprotected shoot tips of Mentha × piperita , 2007, Protoplasma.

[19]  Ramanjulu Sunkar,et al.  Small RNAs as big players in plant abiotic stress responses and nutrient deprivation. , 2007, Trends in plant science.

[20]  Kazuo Shinozaki,et al.  Regulatory metabolic networks in drought stress responses. , 2007, Current opinion in plant biology.

[21]  M. Wise,et al.  The continuing conundrum of the LEA proteins , 2007, Naturwissenschaften.

[22]  D. Blagojević.  Antioxidant systems in supporting environmental and programmed adaptations to low temperatures. , 2007, Cryo letters.

[23]  E. Benson,et al.  Antioxidant status and genotypic tolerance of Ribes in vitro cultures to cryopreservation , 2007 .

[24]  R. Margesin,et al.  Cold-loving microbes, plants, and animals—fundamental and applied aspects , 2007, Naturwissenschaften.

[25]  K. Shinozaki,et al.  Gene networks involved in drought stress response and tolerance. , 2006, Journal of experimental botany.

[26]  G. Volk,et al.  Cryopreservation of Arabidopsis thaliana shoot tips. , 2006, Cryo letters.

[27]  R. Swennen,et al.  Change in sugar, sterol and fatty acid composition in banana meristems caused by sucrose-induced acclimation and its effects on cryopreservation , 2006 .

[28]  K. Shinozaki,et al.  Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. , 2006, Current opinion in plant biology.

[29]  P. Agarwal,et al.  Role of DREB transcription factors in abiotic and biotic stress tolerance in plants , 2006, Plant Cell Reports.

[30]  Kazuo Shinozaki,et al.  Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. , 2006, Annual review of plant biology.

[31]  G. Volk,et al.  Survival of mint shoot tips after exposure to cryoprotectant solution components. , 2006, Cryobiology.

[32]  K. Storey.  Reptile freeze tolerance: metabolism and gene expression. , 2006, Cryobiology.

[33]  Bo Franzén,et al.  Frozen tissue biobanks. Tissue handling, cryopreservation, extraction, and use for proteomic analysis , 2006, Acta oncologica.

[34]  N. Tuteja,et al.  Cold, salinity and drought stresses: an overview. , 2005, Archives of biochemistry and biophysics.

[35]  I. Johnston,et al.  Genomics: applications to Antarctic ecosystems , 2005, Polar Biology.

[36]  B. Fuller,et al.  Cryoprotectants: the essential antifreezes to protect life in the frozen state. , 2004, Cryo letters.

[37]  Chung-Jui Tsai,et al.  Cryopreservation in Populus functional genomics. , 2004, The New phytologist.

[38]  F. Engelmann.  Plant cryopreservation: Progress and prospects , 2004, In Vitro Cellular & Developmental Biology - Plant.

[39]  K. Storey.  Strategies for exploration of freeze responsive gene expression: advances in vertebrate freeze tolerance. , 2004, Cryobiology.

[40]  Satish Kumar,et al.  Cryopreservation of Pinus radiata zygotic embryo cotyledons: effect of storage duration on adventitious shoot formation and plant growth after 2 years in the field , 2004 .

[41]  R. Swennen,et al.  A comparision of four methods for cryopreservation of meristems in banana (Musa spp.). , 2004, Cryo letters.

[42]  M. Uemura,et al.  Modification of the intracellular sugar content alters the incidence of freeze-induced membrane lesions of protoplasts isolated from Arabidopsis thaliana leaves , 2003 .

[43]  M. Thomashow,et al.  Arabidopsis Transcriptome Profiling Indicates That Multiple Regulatory Pathways Are Activated during Cold Acclimation in Addition to the CBF Cold Response Pathway Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1 , 2002, The Plant Cell Online.

[44]  K. Akiyama,et al.  Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. , 2002, The Plant journal : for cell and molecular biology.

[45]  D. Hincha.  Cryoprotectin: a plant lipid-transfer protein homologue that stabilizes membranes during freezing. , 2002, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[46]  B. Reed,et al.  Preculture Conditions Influence Cold Hardiness and Regrowth of Pyrus cordata Shoot Tips After Cryopreservation , 2001 .

[47]  K. Dixon,et al.  Genetic fidelity and viability of Anigozanthos viridis following tissue culture, cold storage and cryopreservation , 2001 .

[48]  D. Hincha,et al.  Non-disaccharide-based mechanisms of protection during drying. , 2001, Cryobiology.

[49]  K. Harding,et al.  Biometric analysis of phenotypic characters of potato shoot-tips recovered from tissue culture, dimethyl sulphoxide treatment and cryopreservation. , 2001, Cryo letters.

[50]  K. Harding,et al.  The use of microsatellite analysis in Solanum tuberosum l. in vitro plantlets derived from cryopreserved germplasm. , 2001, Cryo letters.

[51]  J. Browse,et al.  Cold comfort farm: the acclimation of plants to freezing temperatures. , 2000 .

[52]  J. Verdeil,et al.  Somaclonal variation in oil palm (Elaeis guineensis Jacq.): the DNA methylation hypothesis , 2000, Plant Cell Reports.

[53]  N. Pammenter,et al.  A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms , 1999, Seed Science Research.

[54]  K. Storey.  LIVING IN THE COLD: FREEZE‐INDUCED GENE RESPONSES IN FREEZE‐TOLERANT VERTEBRATES , 1999, Clinical and experimental pharmacology & physiology.

[55]  K. Shinozaki,et al.  Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis , 1998, Plant Cell.

[56]  S. Kobayashi,et al.  Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification , 1990, Plant Cell Reports.

[57]  N. Tyler,et al.  DEHYDRATION OF DORMANT APPLE BUDS AT DIFFERENT STAGES OF COLD ACCLIMATION TO INDUCE CRYOPRESERVABILITY IN DIFFERENT CULTIVARS , 1988 .

[58]  W. Gordon-Kamm,et al.  Lamellar-to-hexagonalII phase transitions in the plasma membrane of isolated protoplasts after freeze-induced dehydration. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[59]  W. Gordon-Kamm,et al.  The behavior of the plasma membrane following osmotic contraction of isolated protoplasts: Implications in freezing injury , 1984, Protoplasma.

[60]  P. Kramer,et al.  Responses of Plants to Environmental Stresses , 1973 .

[61]  Ling-Xiao Liu,et al.  Determination of genetic stability in surviving apple shoots following cryopreservation by vitrification. , 2008, Cryo letters.

[62]  Chhandak Basu.  Gene amplification from cryopreserved Arabidopsis thaliana shoot tips. , 2008, Current issues in molecular biology.

[63]  E. R. Joachim Keller,et al.  Cryopreservation of cold-acclimated mint (Mentha spp.) shoot tips using a simple vitrification protocol. , 2007, Cryo letters.

[64]  J. V. Van Beeumen,et al.  Functional proteome analysis of the banana plant (Musa spp.) using de novo sequence analysis of derivatized peptides. , 2007, Journal of proteome research.

[65]  K. Shinozaki,et al.  Plant gene networks in osmotic stress response: from genes to regulatory networks. , 2007, Methods in enzymology.

[66]  K. Laukens,et al.  Banana (Musa spp.) as a model to study the meristem proteome: Acclimation to osmotic stress , 2007, Proteomics.

[67]  N. Suzuki,et al.  Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction , 2006 .

[68]  M. Uemura,et al.  Responses of the plasma membrane to low temperatures , 2006 .

[69]  R. Swennen,et al.  Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae , 2005 .

[70]  D. Black,et al.  Desiccation and Survival in Plants : Drying without Dying , 2005 .

[71]  E. Kendall,et al.  Cryopreservation of immature spring wheat zygotic embryos using an abscisic acid pretreatment , 2004, Plant Cell Reports.

[72]  M. D. De Proft,et al.  Changes in sugar content and fatty acid composition of in vitro sugar beet shoots after cold acclimation: influence on survival after cryopreservation , 2004, Plant Growth Regulation.

[73]  K. Shinozaki,et al.  Isolation and Functional Analysis of Arabidopsis Stress-Inducible NAC Transcription Factors That Bind to a Drought-Responsive cis-Element in the early responsive to dehydration stress 1 Promoter , 2004 .

[74]  Hugh W. Pritchard,et al.  Drying without dying. , 2002 .

[75]  B. Reed,et al.  Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation , 1999 .

[76]  Y. Amano,et al.  Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification , 1993 .