Characterization of the Promoter of the Mouse Preproorexin Gene

The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between −13 and +112 and between −1,868 and −780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

[1]  S. Kida,et al.  Tight regulation of transgene expression by tetracycline‐dependent activator and repressor in brain , 2005, Genes, brain, and behavior.

[2]  S. Kida,et al.  A BMAL1 mutant with arginine 91 substituted with alanine acts as a dominant negative inhibitor. , 2004, Gene.

[3]  A. Cadete-Leite,et al.  NGF and NT-3 exert differential effects on the expression of neuropeptides in the suprachiasmatic nucleus of rats withdrawn from ethanol treatment , 2003, Brain Research.

[4]  M. Yanagisawa,et al.  Sleep, feeding, and neuropeptides: roles of orexins and orexin receptors , 2002, Current Opinion in Neurobiology.

[5]  T. Sakurai,et al.  The Human Prepro-orexin Gene Regulatory Region That Activates Gene Expression in the Lateral Region and Represses It in the Medial Regions of the Hypothalamus* , 2002, The Journal of Biological Chemistry.

[6]  T. Sakurai,et al.  Structure and Function of Human Prepro-orexin Gene* , 1999, The Journal of Biological Chemistry.

[7]  H. Yamazaki,et al.  Molecular Cloning of a Novel Human Collectin from Liver (CL-L1)* , 1999, The Journal of Biological Chemistry.

[8]  T. Murata,et al.  Promoter Characterization of the Rat Gene for Ca2+-binding Protein Regucalcin , 1999, The Journal of Biological Chemistry.

[9]  T. Lundeberg,et al.  Monosodium glutamate increases NGF and NPY concentrations in rat hypothalamus and pituitary , 1995, Neuroreport.

[10]  G. Taglialatela,et al.  Nerve growth factor modulates the activation of the hypothalamo-pituitary-adrenocortical axis during the stress response. , 1991, Endocrinology.

[11]  E. Alleva,et al.  Nerve growth factor mRNA and protein increase in hypothalamus in a mouse model of aggression. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[12]  S. Kida,et al.  PLCγ2 Activates CREB-dependent Transcription in PC12 Cells Through Phosphorylation of CREB at Serine 133 , 2005, Cytotechnology (Dordrecht).

[13]  Jon T. Willie,et al.  To eat or to sleep? Orexin in the regulation of feeding and wakefulness. , 2001, Annual review of neuroscience.

[14]  S. Carr,et al.  Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. , 1998, Cell.

[15]  S. Smale,et al.  DNA sequence requirements for transcriptional initiator activity in mammalian cells. , 1994, Molecular and cellular biology.