论文信息 - A RAPID METHOD FOR ESTIMATING VIABILITY IN DESICCATED CELLS OF THE BIOCONTROL AGENT TSUKAMURELLA PAUROMETABOLA C-924

A RAPID METHOD FOR ESTIMATING VIABILITY IN DESICCATED CELLS OF THE BIOCONTROL AGENT TSUKAMURELLA PAUROMETABOLA C-924

A new method for the rapid estimation of viability in anhydrobiotic cells of the biocontrol agent Tsukamurella paurometabola C-924 was developed. The method is based on the absorbance due to hydrogen sulfide production. A linear model was obtained by plotting decimal logarithm of the corrected absorbance at 670 nm (log(Abscorrected)) versus the decimal logarithm of cul- turable cells (log(Xv)) (r 2 = 0.9428). After comparing the results obtained by the proposed method to the ones obtained by the plate counting technique, no significant difference was observed in the number of culturable cells. The new technique, which is faster than plate counting, permits the estimation of biologic activity in anhydrobiotic cells of the strain C-924 in only 2 h.

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