A capacitive immunosensor using on-chip electrolytic pumping and magnetic washing techniques for point-of-care applications

This work presents a capacitive immunosensor using on-chip electrolytic pumping and magnetic washing techniques. The proposed device possesses the advantages such as simple operation, low power consumption, and portability. The proposed device was fabricated using typical micromachining process, and is suitable for mass-production. We also demonstrated the detection of N-Terminal pro-brain-Type natriuretic peptide (NT-proBNP) using the fabricated device integrated with a CMOS capacitance sensing chip. The proposed device potentially can be used as a portable system for point-of-care applications.

[1]  A. Helmicki,et al.  An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities. , 2002, Lab on a chip.

[2]  S. Gambini,et al.  Design Considerations for CMOS-Integrated Hall-Effect Magnetic Bead Detectors for Biosensor Applications , 2013, Journal of Microelectromechanical Systems.

[3]  Nai-Kuan Chou,et al.  A Wireless Bio-MEMS Sensor for C-Reactive Protein Detection Based on Nanomechanics , 2006, ISSCC.

[4]  Cheng-Hsien Liu,et al.  An Electrolysis-Bubble-Actuated Micropump Based on the Roughness Gradient Design of Hydrophobic Surface , 2006 .

[5]  Hyun C. Yoon,et al.  An electrochemical immunosensing lab-on-a-chip integrated with latch mechanism for hand operation , 2009 .

[6]  Cheng-Hsien Liu,et al.  A bubble-activated micropump with high-frequency flow reversal , 2010 .

[7]  Luke P. Lee,et al.  Innovations in optical microfluidic technologies for point-of-care diagnostics. , 2008, Lab on a chip.

[8]  T. Cui,et al.  An electric detection of immunoglobulin G in the enzyme-linked immunosorbent assay using an indium oxide nanoparticle ion-sensitive field-effect transistor , 2011 .

[9]  A. Turner,et al.  Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays. , 2001, Biosensors & bioelectronics.

[10]  Chong H. Ahn,et al.  A large area nano-gap interdigitated electrode array on a polymer substrate as a disposable nano-biosensor , 2013 .

[11]  Yong-Jun Kim,et al.  Neisseria Meningitidis Detection Based on a Microcalorimetric Biosensor With a Split-Flow Microchannel , 2008, Journal of Microelectromechanical Systems.

[12]  An-Yu Chang,et al.  A CMOS magnetic microbead-based capacitive biosensor array with on-chip electromagnetic manipulation. , 2013, Biosensors & bioelectronics.

[13]  Babak Ziaie,et al.  Hydrogel-based microsensors for wireless chemical monitoring , 2009, Biomedical microdevices.