YAC transgene‐mediated olfactory receptor gene choice

In the mouse, individual olfactory neurons express one of a thousand distinct olfactory receptor genes. Furthermore, only one allele of the expressed gene is transcribed. This phenomenon, random allelic inactivation, along with the observation that the olfactory receptor genes reside in large chromosomal arrays, suggests a role for long‐range gene regulation in olfactory receptor gene choice. We have constructed a 300‐kb yeast artificial chromosome (YAC) transgene in which a single receptor gene is marked while maintaining its coding region. This 300‐kb piece of DNA functions as an independent olfactory receptor gene locus in directing olfactory receptor gene choice in both the olfactory system and the accessory olfactory system (vomeronasal organ, VNO). Furthermore, the transgene, like endogenous olfactory receptor loci, is subject to allelic inactivation. Dev Dyn;217:225–231. © 2000 Wiley‐Liss, Inc.

[1]  I. Rodriguez,et al.  Variable Patterns of Axonal Projections of Sensory Neurons in the Mouse Vomeronasal System , 1999, Cell.

[2]  R. Axel,et al.  A Map of Pheromone Receptor Activation in the Mammalian Brain , 1999, Cell.

[3]  L. Buck,et al.  Combinatorial Receptor Codes for Odors , 1999, Cell.

[4]  R. Axel,et al.  Odorant Receptors Govern the Formation of a Precise Topographic Map , 1998, Cell.

[5]  Randall R. Reed,et al.  Tissue and Zonal-Specific Expression of an Olfactory Receptor Transgene , 1998, The Journal of Neuroscience.

[6]  L. Buck,et al.  A Multigene Family Encoding a Diverse Array of Putative Pheromone Receptors in Mammals , 1997, Cell.

[7]  C. Dulac,et al.  A Novel Family of Putative Pheromone Receptors in Mammals with a Topographically Organized and Sexually Dimorphic Distribution , 1997, Cell.

[8]  N. Ryba,et al.  A New Multigene Family of Putative Pheromone Receptors , 1997, Neuron.

[9]  R. Jaenisch,et al.  A method for high efficiency YAC lipofection into murine embryonic stem cells. , 1996, Nucleic acids research.

[10]  Richard Axel,et al.  Visualizing an Olfactory Sensory Map , 1996, Cell.

[11]  R. Axel,et al.  A novel family of genes encoding putative pheromone receptors in mammals , 1995, Cell.

[12]  R. Axel,et al.  The molecular logic of smell. , 1995, Scientific American.

[13]  Linda B. Buck,et al.  Information coding in the olfactory system: Evidence for a stereotyped and highly organized epitope map in the olfactory bulb , 1994, Cell.

[14]  Richard Axel,et al.  Topographic organization of sensory projections to the olfactory bulb , 1994, Cell.

[15]  Gordon M. Shepherd,et al.  Discrimination of molecular signals by the olfactory receptor neuron , 1994, Neuron.

[16]  I. Simon,et al.  Allelic inactivation regulates olfactory receptor gene expression , 1994, Cell.

[17]  Richard Axel,et al.  Spatial segregation of odorant receptor expression in the mammalian olfactory epithelium , 1993, Cell.

[18]  D. J. Driscoll,et al.  Allele-specific replication timing of imprinted gene regions , 1993, Nature.

[19]  Linda B. Buck,et al.  A zonal organization of odorant receptor gene expression in the olfactory epithelium , 1993, Cell.

[20]  D. Ward,et al.  Delineation of DNA replication time zones by fluorescence in situ hybridization. , 1992, The EMBO journal.

[21]  R. Axel,et al.  A novel multigene family may encode odorant receptors: A molecular basis for odor recognition , 1991, Cell.

[22]  V. Ingram,et al.  Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases. , 1988, Journal of molecular biology.

[23]  G. Kollias,et al.  Position-independent, high-level expression of the human β-globin gene in transgenic mice , 1987, Cell.

[24]  S. Tonegawa,et al.  Somatic generation of antibody diversity. , 1976, Nature.

[25]  J. H. Taylor,et al.  Asynchronous Duplication of Chromosomes in Cultured Cells of Chinese Hamster , 2003 .

[26]  J. Knoll,et al.  Allele specificity of DNA replication timing in the Angelman/Prader–Willi syndrome imprinted chromosomal region , 1994, Nature Genetics.

[27]  J B Lawrence,et al.  Fluorescent detection of nuclear RNA and DNA: implications for genome organization. , 1991, Methods in cell biology.

[28]  B. Trask.  Chapter 1 DNA Sequence Localization in Metaphase and Interphase Cells by Fluorescence in Situ Hybridization , 1991 .

[29]  R. Rothstein.  18 – One-Step Gene Disruption in Yeast , 1989 .

[30]  R. Rothstein.  One-step gene disruption in yeast. , 1983, Methods in enzymology.