Three novel xylosides, cimicifugosides H-1(1), -3(2) and -4(3), were isolated from a commercial Cimicifuga Rhizome. Their structures were determined on the basis of chemical and spectrometric evidence including an X-ray crystallographic analysis. The xyloside 1 is 3-O-xyloside of (20R, 24R)-24, 25-epoxy-3β, 11β-dihydroxy-9, 19-cyclolanost-7-ene-16, 23-dione, while 2 and 3 are 3-O-xylosides of 25, 26, 27-trinor-derivatives from the genin of 1.