Comparison of Rous Sarcoma Virus-Specific Deoxyribonucleic Acid Polymerases in Virions of Rous Sarcoma Virus and in Rous Sarcoma Virus-Infected Chicken Cells

Labeled virions of Rous sarcoma virus (RSV) were disrupted with detergent and analyzed on equilibrium sucrose density gradients. A core fraction at a density of approximately 1.24 g/cc contained all of the 3H-uridine label and about 30% of the 3H-leucine label from the virions. Endogenous viral deoxyribonucleic acid (DNA) polymerase activity was only found in the same location. Additional ribonucleic acid (RNA)- and DNA-dependent DNA polymerase activities were found at the top of the gradients. RNA-dependent and DNA-dependent DNA polymerase activities were also found in RSV-converted chicken cells. Particles containing these activities were released from cells by detergent and were shown to contain viral RNA. These particles were analyzed on equilibrium sucrose density gradients and were found to have densities different from virion cores.

[1]  S. Mizutani,et al.  DNA ligase and exonuclease activities in virions of rous sarcoma virus. , 1971, Nature: New biology.

[2]  E. Scolnick,et al.  RNA Dependent DNA Polymerase Activity in Mammalian Cells , 1971, Nature.

[3]  R. Gallo,et al.  RNA Dependent DNA Polymerase of Human Acute Leukaemic Cells , 1970, Nature.

[4]  E. Scolnick,et al.  Separation of RNA-dependent DNA Polymerase Activity from the Murine Leukaemia Virion , 1970, Nature.

[5]  E. Delain,et al.  Coiled structure of the nucleocapsid of avian myeloblastosis virus. , 1970, The Journal of general virology.

[6]  A. Burny,et al.  DNA-directed DNA Polymerase Activity in Oncogenic RNA Viruses , 1970, Nature.

[7]  J. Bader,et al.  Characteristics of cores of avian leuko-sarcoma viruses. , 1970, Virology.

[8]  H. Temin,et al.  Studies on carcinogenesis by avian sarcoma viruses: VIII. Glycolysis and cell multiplication , 1968 .

[9]  H. Temin.  Studies on carcinogenesis by avian sarcoma viruses. V. Requirement for new DNA synthesis and for cell division , 1967 .

[10]  H. Bauer,et al.  Origin of group-specific antigen of chicken leukosis viruses. , 1966, Virology.

[11]  G. de‐Thé,et al.  Structure of a murine leukemia virus after disruption with tween-ether and comparison with two myxoviruses. , 1966, Virology.

[12]  W. Robinson,et al.  The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[13]  B. O'brien,et al.  THE PARTIAL CYTOLYSIS OF THE AMPHIBIAN ERYTHROCYTE AND LIVER PARENCYHMA CELL BY A NON-IONOGENIC SURFACE ACTIVE AGENT , 1964, The Journal of cell biology.

[14]  S. Mizutani,et al.  RNA-dependent DNA polymerase in virions of Rous sarcoma virus. , 1970, Nature.

[15]  H. Temin,et al.  Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses. , 1970, Virology.