MITOTIC RESPONSES OF THE COMPENSATING RAT KIDNEY TO INJECTIONS OF TISSUE HOMOGENATES.

Summary Mitotic activity in the remaining kidneys of unilaterally nephrectomized rats reaches a peak about 48 hours after operation. To test for the possible existence of tissue-specific growth-regulating factors, such rats were given injections of various tissue homogenates ca . 30 hours after uninephrectomy. Intraperitoneal administration of homogenates of fresh, cooked, or frozen kidneys, or suspensions of trypsin-dissociated kidneys, all reduced the 48-hour mitotic peak of hyperplastic kidneys by approximately 50 per cent. Saline-injected controls were insignificantly altered. Subcutaneous injections of frozen kidney homogenates did not adversely affect compensatory renal hyperplasia. Intraperitoneal injections of fresh liver, testis, spleen, and blood homogenates inhibited renal mitosis as effectively as did kidney homogenates. Blood plasma from normal or uninephrectomized rats, however, were not effective. Egg albumin suppressed mitosis, but egg yolk did not. In view of these failures to demonstrate a tissue-specific effect of homogenates on renal mitosis, together with similar discrepancies in comparable studies of liver, the existence of growth-regulating agents related to organ mass per se, and distinct from other functional demands, must remain problematical.

[1]  P. Tardent,et al.  AN ANALYSIS OF THE INITIAL REACTION IN THE SEQUENCE RESULTING IN HOMOLOGOUS SPLENOMEGALY IN THE CHICK EMBRYO , 1962 .

[2]  W. Bullough.  THE CONTROL OF MITOTIC ACTIVITY IN ADULT MAMMALIAN TISSUES , 1962, Biological reviews of the Cambridge Philosophical Society.

[3]  G. E. Williams.  Some aspects of compensatory hyperplasia of the kidney. , 1961, British journal of experimental pathology.

[4]  N. L. Trotter.  The effect of partial hepatectomy on subcutaneously transplanted hepatomas in mice. , 1961, Cancer research.

[5]  A. Lahtiharju.  Influence of autolytic and necrotic liver tissue on liver regeneration in rat. , 1961, Acta pathologica et microbiologica Scandinavica. Supplement.

[6]  R. Goss,et al.  Physiological factors affecting compensatory renal hyperplasia in the rat. , 1960, The Journal of experimental zoology.

[7]  H. Stich.  REGULATION OF MITOTIC RATE IN MAMMALIAN ORGANISMS , 1960 .

[8]  G. Tumanishvili.  Stimulating action of tissue extracts on regenerative processes. , 1960, Journal of embryology and experimental morphology.

[9]  P. J. Alten.  The Effects of Chorioallantoic Grafts on the developing Chick Embryo II. Studies of Adult Antigens in the Duodenum and Spleen , 1959 .

[10]  P. V. van Alten,et al.  The effects of chorioallantoic grafts on the developing chick embryo. I. Studies on weight and histology of homologous and heterologous tissues. , 1959, Journal of embryology and experimental morphology.

[11]  M. Swann.  The control of cell division: a review. II. Special mechanisms. , 1958, Cancer research.

[12]  K. Paschkis.  Growth-promoting factors in tissues: a review. , 1958, Cancer research.

[13]  H. Stich,et al.  The presence of a mitosis inhibitor in the serum and liver of adult rats. , 1958, Canadian journal of biochemistry and physiology.

[14]  Rose Sm.  Failure of self-inhibition in tumors. , 1958 .

[15]  Y. Croisille.  Action de différents extraits d'organes sur l'embryon de poulet et sur des organes embryonnaires cultivés in vitro. , 1958 .

[16]  S. Rose.  Failure of self-inhibition in tumors. , 1958, Journal of the National Cancer Institute.

[17]  J. L. Kavanau,et al.  A MODEL OF GROWTH AND GROWTH CONTROL IN MATHEMATICAL TERMS , 1957, The Journal of general physiology.

[18]  A. Larmo,et al.  Influence of intraperitoneally injected homogenates of the outer orbital gland and the liver on RNA and DNA in these organs in rats. , 1957, Experimental cell research.

[19]  M. Abercrombie.  Localized formation of new tissue in an adult mammal. , 1957, Symposia of the Society for Experimental Biology.

[20]  K. Blomqvist.  Growth stimulation in the liver and tumour development following intraperitoneal injections of liver homogenates in the rat. , 1957, Acta pathologica et microbiologica Scandinavica. Supplementum.

[21]  H. Mahler,et al.  Influence of adult tissue homogenates on formation of similar embryonic proteins. , 1956, Science.

[22]  H. Saetren.  A principle of auto-regulation of growth; production of organ specific mitose-inhibitors in kidney and liver. , 1956, Experimental cell research.

[23]  R. A. Malmgren.  Observations on a liver mitotic stimulant present in tumor tissue. , 1956, Cancer research.

[24]  A. Telkka,et al.  Influence of thymus suspension on mitotic rate in the thymus, liver, and outer orbital gland of rat. , 2009, Acta pathologica et microbiologica Scandinavica.

[25]  G. Andres.  Growth reactions of mesonephros and liver to intravascular injections of embryonic liver and kidney suspensions in the chick embryo , 1955 .

[26]  J. Ebert.  THE EFFECTS OF CHORIOALLANTOIC TRANSPLANTS OF ADULT CHICKEN TISSUES ON HOMOLOGOUS TISSUES OF THE HOST CHICK EMBRYO. , 1954, Proceedings of the National Academy of Sciences of the United States of America.

[27]  H. Teir,et al.  Mitotic activity and growth factors in the liver of the white rat. , 1953, Experimental cell research.

[28]  H. Teir.  Experimental alterations of cell size and mitotic activity in the outer orbital gland of the white rat. IV. Influence of parenterally applied extracts of outer orbital gland. , 2009, Acta pathologica et microbiologica Scandinavica.

[29]  E. Annau,et al.  Increased weight and mitotic activity in the liver of tumor-bearing rats and mice. , 1951, Cancer research.

[30]  E. Leduc,et al.  Mitotic rate in mouse liver following intraperitoneal injection of liver, kidney and egg yolk , 1947, The Anatomical record.

[31]  A. Walker,et al.  EFFECT OF LIVER FRACTIONS ON MITOSIS IN REGENERATING LIVER , 1945 .

[32]  F. A. Mcjunkin,et al.  Effect of Macerated Kidney on the Mitotic Rate of Kidney Epithelium , 1932 .