Automatic-identification of spots in biochip images

Automatic-identification of spots is the key for automatic-extracting information from biochip images. A new approach for automatic-identification is proposed based on the relations of spots, noises and backgrounds. Morphological and mean filtering was used to filter and enhance images and estimate intensity of background. Spectrum of power was used to correct tilting and perform spots gridding automatically. Considering intensity and standard deviation of edge, a new technique to correct center and radius was introduced. The approach has been tested in a variety of biochip images, and better resistant performance of noises and identification of weak signals are realized. It can automatic-identify spots quickly and accurately.