Calibration Curve Fitting

This chapter explains the theory and practice of immunoassay calibration curve fitting techniques. The nature and characteristics of the immunoassay dose-response curve are discussed and the general principles involved in finding a suitable response transformation, including the importance of the response-error relationship. Curve-fitting methods are explained, starting with early approaches that are seldom used now. Regression and curve-fit metrics are discussed in detail, with extensive coverage of 4-parameter and 5-parameter logistic methods, which are most commonly used. The importance of calibrator positioning, understanding error profiles and the implications of outliers are all discussed and explained. There is also a section on commercial systems involving factory-set master curves and adjusters run in the user laboratory.

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