Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5'-phosphate.

Pyridoxal 5’-phosphate (PLP, 1) is the biologically active form of vitamin B6, and is essential for performing the chemistry of primary metabolism in all varieties of life. The most common pathway for the biosynthesis of PLP involves only two enzymes, Pdx1 (SNZ) and Pdx2 (SNO). This biosynthetic activity has recently been reconstituted in vitro and was shown to use ribose-5-phosphate (R5P, 2), glutamine 3, and glyceraldehyde-3-phosphate (G3P, 4) as substrates (Scheme 1).

[1]  Ivo Tews,et al.  Thermodynamic characterization of the protein-protein interaction in the heteromeric Bacillus subtilis pyridoxalphosphate synthase. , 2007, Biochemistry.

[2]  I. Tews,et al.  Functional Analysis of PDX2 from Arabidopsis, a Glutaminase Involved in Vitamin B6 Biosynthesis1[W][OA] , 2007, Plant Physiology.

[3]  N. Amrhein,et al.  Reaction Mechanism of Pyridoxal 5′-Phosphate Synthase , 2007, Journal of Biological Chemistry.

[4]  Elizabeth E. Rueschhoff,et al.  Regulation of the Arabidopsis thaliana vitamin B6 biosynthesis genes by abiotic stress. , 2007, Plant physiology and biochemistry : PPB.

[5]  Ivo Tews,et al.  Structure of a bacterial pyridoxal 5′-phosphate synthase complex , 2006, Proceedings of the National Academy of Sciences.

[6]  T. Begley,et al.  Structural insights into the mechanism of the PLP synthase holoenzyme from Thermotoga maritima. , 2006, Biochemistry.

[7]  S. Müller,et al.  Vitamin B6 Biosynthesis by the Malaria Parasite Plasmodium falciparum , 2006, Journal of Biological Chemistry.

[8]  N. Amrhein,et al.  Vitamin B6 biosynthesis in higher plants. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[9]  N. Amrhein,et al.  On the Two Components of Pyridoxal 5′-Phosphate Synthase from Bacillus subtilis* , 2005, Journal of Biological Chemistry.

[10]  F. McLafferty,et al.  Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway. , 2005, Journal of the American Chemical Society.

[11]  R. D. Walter,et al.  Analysis of the Vitamin B6 Biosynthesis Pathway in the Human Malaria Parasite Plasmodium falciparum* , 2005, Journal of Biological Chemistry.

[12]  M. Ehrenshaft,et al.  Functional complementation between the PDX1 vitamin B6 biosynthetic gene of Cercospora nicotianae and pdxJ of Escherichia coli , 2004, FEBS letters.

[13]  J. Nikawa,et al.  Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae. , 2004, European journal of biochemistry.

[14]  Tadhg P Begley,et al.  Three-dimensional Structure of YaaE from Bacillus subtilis, a Glutaminase Implicated in Pyridoxal-5′-phosphate Biosynthesis* , 2004, Journal of Biological Chemistry.

[15]  M. Daub,et al.  Isolation of PDX2, a Second Novel Gene in the Pyridoxine Biosynthesis Pathway of Eukaryotes, Archaebacteria, and a Subset of Eubacteria , 2001, Journal of bacteriology.