Fourier-transform infrared spectroscopic study of the interactions of selenium species with living bacterial cells

A study of the interactions of several selenium species with living bacterial cells was carried out by Fourier-transform infrared (FT-IR) spectroscopy. Bacterial cells consisted of an Escherichia coli strain (K-12) cultivated in a growth medium based on glucose contaminated with selenium species. Equilibrium between the analyte in the solution and the extraction medium was established, and then the effects of selenium species upon the external membrane of the living bacterial cells were characterized by performing FT-IR spectroscopy of whole cells. The presence of the toxicants at various concentrations in the culture medium had an effect on the FT-IR spectra, and the concentration of the selenium species was determined directly in the biomass by FT-IR spectroscopy. The intensity ratios between several absorption lines, which varied as a function of the concentration of the selenium species, were used as the analytical signal.

[1]  L. C. Robles,et al.  Stability of bacterium-mercury complexes and speciation of soluble inorganic mercury species , 1996 .

[2]  E. Papavassiliou,et al.  Phosphodiester Stretching Bands in the Infrared Spectra of Human Tissues and Cultured Cells , 1991 .

[3]  B. Rigas,et al.  Human colon adenocarcinoma cell lines display infrared spectroscopic features of malignant colon tissues. , 1992, Cancer research.

[4]  D. Anderson,et al.  Direct Determination of Palladium and Cobalt in Phenol by Atomic Spectroscopy , 1993 .

[5]  Marc Meurens,et al.  Breast cancer detection by Fourier transform infrared spectrometry , 1996 .

[6]  K. Fuwa,et al.  Selenium and arsenic in biology: their chemical forms and biological functions. , 1992, Advances in biophysics.

[7]  R. Jakobsen,et al.  Infrared Spectra-Structure Correlations and Adsorption Behavior for Helix Proteins , 1990 .

[8]  Harald Labischinski,et al.  Microbiological characterizations by FT-IR spectroscopy , 1991, Nature.

[9]  M. Manfait,et al.  FT-IR spectroscopy as an emerging method for rapid characterization of microorganisms. , 1998, Cellular and molecular biology.

[10]  F. Mauro,et al.  A New Approach to the Study of Human Solid Tumor Cells by Means of FT-IR Microspectroscopy , 1990 .

[11]  Chris W. Brown,et al.  Screening Pap Smears with Near-Infrared Spectroscopy , 1995 .

[12]  L. C. Robles,et al.  Determination of cadmium in biological and environmental samples by slurry electrothermal atomic absorption spectrometry. , 1995, Talanta.

[13]  W. Wolf,et al.  Microbiological assay for chemical species of selenium in foods utilizing Escherichia coli formate dehydrogenase , 1993 .

[14]  E H Holmes,et al.  The etiology and prediction of breast cancer. Fourier transform‐infrared spectroscopy reveals progressive alterations in breast DNA leading to a cancer‐like phenotype in a high proportion of normal women , 1995, Cancer.

[15]  A. Aller.  Speciation of selenomethionine and selenourea using living bacterial cells , 1998 .

[16]  Thomas Udelhoven,et al.  Development of a Hierarchical Classification System with Artificial Neural Networks and FT-IR Spectra for the Identification of Bacteria , 2000 .

[17]  A. Aller,et al.  Speciation of mercury, methylmercury, ethylmercury and phenylmercury by Fourier transform infrared spectroscopy of whole bacterial cells , 2001 .

[18]  Max Diem,et al.  Infrared spectroscopy of cultured cells: II. Spectra of exponentially growing, serum-deprived and confluent cells , 2003 .

[19]  Royston Goodacre,et al.  Rapid Differentiation of Closely RelatedCandida Species and Strains by Pyrolysis-Mass Spectrometry and Fourier Transform-Infrared Spectroscopy , 1998, Journal of Clinical Microbiology.

[20]  V. Majidi,et al.  Evaluation of the metal uptake of several algae strains in a multicomponent matrix utilizing inductively coupled plasma emission spectrometry. , 1989, Analytical chemistry.

[21]  Dieter Naumann,et al.  Identification of some bacterial cell components by FT-IR spectroscopy , 1995 .

[22]  Harald Labischinski,et al.  The rapid differentiation and identification of pathogenic bacteria using Fourier transform infrared spectroscopic and multivariate statistical analysis , 1988 .

[23]  D. Naumann,et al.  Classification and Identification of Enterococci: a Comparative Phenotypic, Genotypic, and Vibrational Spectroscopic Study , 2001, Journal of Clinical Microbiology.

[24]  D. Naumann,et al.  Classification and identification of bacteria by Fourier-transform infrared spectroscopy. , 1991, Journal of general microbiology.

[25]  Henry H. Mantsch,et al.  Molecular spectroscopy in biodiagnostics (from Hippocrates to Herschel and beyond) , 1995 .