Mortalin imaging in normal and cancer cells with quantum dot immuno-conjugates

ABSTRACTQuantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine, and have several predictive advantages. These include their i) broad absorption spectra allowing visualization with single light source, ii) exceptional photo-stability allowing long term studies and iii) narrow and symmetrical emission spectrum that is controlled by their size and material composition. These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source, their simultaneous resolution and visualization as different colors. At present there are only a few studies that have tested quantum dots in cellular imaging. We describe here the use of quantum dots in mortalin imaging of normal and cancer cells. Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable.

[1]  A. Prokop.  Bioartificial Organs in the Twenty‐first Century , 2001 .

[2]  J. Matthew Mauro,et al.  Long-term multiple color imaging of live cells using quantum dot bioconjugates , 2003, Nature Biotechnology.

[3]  Michele Follen,et al.  Real-time vital optical imaging of precancer using anti-epidermal growth factor receptor antibodies conjugated to gold nanoparticles. , 2003, Cancer research.

[4]  Xunbin Wei,et al.  Selective cell targeting with light-absorbing microparticles and nanoparticles. , 2003, Biophysical journal.

[5]  M. Bruchez,et al.  Lighting up cells with quantum dots. , 2003, BioTechniques.

[6]  R. Reddel,et al.  Selective toxicity of MKT-077 to cancer cells is mediated by its binding to the hsp70 family protein mot-2 and reactivation of p53 function. , 2000, Cancer research.

[7]  Christopher G Thanos,et al.  Nanotechnology and medicine , 2003, Expert opinion on biological therapy.

[8]  A Curtis,et al.  Nantotechniques and approaches in biotechnology. , 2001, Trends in biotechnology.

[9]  S. Kaul,et al.  Differential subcellular distribution of mortalin in mortal and immortal mouse and human fibroblasts. , 1993, Experimental cell research.

[10]  O. Pereira-smith,et al.  Mortalin: present and prospective , 2002, Experimental Gerontology.

[11]  Philippe Menei,et al.  Drug targeting into the central nervous system by stereotactic implantation of biodegradable microspheres. , 1994 .

[12]  Bing Xu,et al.  Using biofunctional magnetic nanoparticles to capture gram-negative bacteria at an ultra-low concentration. , 2003, Chemical communications.

[13]  S. Kaul,et al.  An Hsp70 family chaperone, mortalin/mthsp70/PBP74/Grp75: what, when, and where? , 2002, Cell stress & chaperones.

[14]  S. Kaul,et al.  5-Bromodeoxyuridine induces senescence-like phenomena in mammalian cells regardless of cell type or species. , 1999, Journal of biochemistry.

[15]  T. Kawai,et al.  DNA-directed magnetic network formations with ferromagnetic nanoparticles. , 2002, Journal of nanoscience and nanotechnology.

[16]  J. Barhen,et al.  Covalent attachment of gold nanoparticles to DNA templates. , 2002, Journal of nanoscience and nanotechnology.

[17]  S. Kaul,et al.  Identification of a novel member of mouse hsp70 family. Its association with cellular mortal phenotype. , 1993, The Journal of biological chemistry.

[18]  J. R. Smith,et al.  Extramitochondrial localization of mortalin/mthsp70/PBP74/GRP75. , 2000, Biochemical and biophysical research communications.

[19]  Mark L Brongersma,et al.  Nanoshells: gifts in a gold wrapper , 2003, Nature materials.

[20]  Ralph Weissleder,et al.  Noninvasive detection of clinically occult lymph-node metastases in prostate cancer. , 2003, The New England journal of medicine.

[21]  Ralph Weissleder,et al.  Viral-induced self-assembly of magnetic nanoparticles allows the detection of viral particles in biological media. , 2003, Journal of the American Chemical Society.

[22]  Atsushi Arakaki,et al.  Single‐nucleotide polymorphism analysis using fluorescence resonance energy transfer between DNA‐labeling fluorophore, fluorescein isothiocyanate, and DNA intercalator, POPO‐3, on bacterial magnetic particles , 2003, Biotechnology and bioengineering.

[23]  A. Prokop.  Bioartificial organs in the twenty-first century: nanobiological devices. , 2001, Annals of the New York Academy of Sciences.

[24]  M. Bruchez,et al.  Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots , 2003, Nature Biotechnology.

[25]  Jayanth Panyam,et al.  Biodegradable nanoparticles for drug and gene delivery to cells and tissue. , 2003, Advanced drug delivery reviews.