Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source.

OBJECTIVE For in vitro tissue engineering in urology, stem cells are commonly isolated from tissue specimens obtained during open or endoscopic surgery. Within the context of the present study our aim was to isolate cells from human urine by an alternative and safe technique rather than using the indicated method. MATERIAL AND METHODS After human urine samples had been collected from young and healthy donors via urethral catheterization, cells were precipitated by centrifugation and cultured. Following this isolation procedure, cells were characterized by immunocytochemical method using specific antibodies. RESULTS When these cells were characterized by immunocytochemical methods using specific antibodies some of them were positive for mesenchymal stem cell marker CD90 while the others were labelled with urothelial marker cytokeratin 7. When all these results were taken into consideration, urothelial cells together with stem cells were observed in the urine- derived cell population. CONCLUSION According to the results obtained from this study human urine may be preferred as an alternative stem cell and urothelial cell source in that this method is and easily reproducible non-invasive method.

[1]  Jia Li,et al.  Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells , 2016, Molecular and Cellular Endocrinology.

[2]  S. Chun,et al.  Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation, Immune Modulation and Multi Differentiation , 2015, Journal of Korean medical science.

[3]  M. Pokrywczyńska,et al.  Urine--a waste or the future of regenerative medicine? , 2015, Medical hypotheses.

[4]  Da-nian Qin,et al.  Urine-derived stem cells for potential use in bladder repair , 2014, Stem Cell Research & Therapy.

[5]  A. Atala,et al.  Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours , 2013, PloS one.

[6]  J. Adamowicz,et al.  Urine is a highly cytotoxic agent: does it influence stem cell therapies in urology? , 2012, Transplantation proceedings.

[7]  Ji-Seon Lee,et al.  Characterization of urine-derived cells from upper urinary tract in patients with bladder cancer. , 2012, Urology.

[8]  M. P,et al.  Adult Mesenchymal Stem Cells and Cell Surface Characterization - A Systematic Review of the Literature , 2011, The open orthopaedics journal.

[9]  Yan Liu,et al.  Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering. , 2011, Biomaterials.

[10]  A. Atala,et al.  Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion. , 2010, Biomaterials.

[11]  Anthony Atala,et al.  Urine derived cells are a potential source for urological tissue reconstruction. , 2008, The Journal of urology.

[12]  Magdalena Fossum,et al.  ISOLATION AND IN VITRO CULTIVATION OF HUMAN UROTHELIAL CELLS FROM BLADDER WASHINGS OF ADULT PATIENTS AND CHILDREN , 2003, Scandinavian journal of plastic and reconstructive surgery and hand surgery.

[13]  Xiaobo Zhou,et al.  Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors. , 2013, Biomaterials.