Effects of growth with ethanol on fermentation and membrane fluidity of Saccharomyces cerevisiae

Saccharomyces cerevisiae HSc was grown with ethanol at concentrations up to 10% (v/v). The immediate effects of additions of externally added ethanol on CO2 production and O2 consumption of washed organisms were studied by stopped‐flow membrane inlet quadrupole mass spectrometry. Fermentative activities of organisms grown with ethanol (0–5% v/v) showed similar sensitivities to inhibition by ethanol, whereas those grown with 10% (v/v) ethanol had become protected and were markedly less sensitive.

[1]  C. Grant,et al.  Ganglioside headgroup dynamics. , 1980, Biochimica et biophysica acta.

[2]  W. Gray,et al.  RELATION OF MOLECULE SIZE AND STRUCTURE TO ALCOHOL INHIBITION OF GLUCOSE UTILIZATION BY YEAST , 1956, Journal of bacteriology.

[3]  L. Ingram,et al.  Effects of ethanol on the Escherichia coli plasma membrane , 1984, Journal of bacteriology.

[4]  C. Leão,et al.  Effects of ethanol and other alkanols on the ammonium transport system of Saccharomyces cerevisiae. , 1982 .

[5]  Rodney P. Jones,et al.  Biological principles for the effects of ethanol , 1989 .

[6]  D Lloyd,et al.  Glycolysis and respiration in yeasts. The Pasteur effect studied by mass spectrometry. , 1983, The Biochemical journal.

[7]  H. Degn,et al.  Stopped flow mass spectrometry: applications to the carbonic anhydrase reaction. , 1986, Journal of biochemical and biophysical methods.

[8]  Peter F. Knowles,et al.  Magnetic Resonance of Biomolecules , 1976 .

[9]  K. Ohta,et al.  Effects of phosphatidylcholine or ergosteryl oleate on physiological properties of Saccharomyces sake , 1980 .

[10]  L. Ingram,et al.  Effects of alcohols on micro-organisms. , 1984, Advances in microbial physiology.

[11]  D. Lloyd,et al.  The Pasteur effect in yeasts: Mass spectrometric monitoring of oxygen uptake, and carbon dioxide and ethanol production , 1987 .

[12]  N. Uden.  Effects of Ethanol on the Temperature Relations of Viability and Growth in Yeast , 1983 .

[13]  W. M. Ingledew,et al.  Ethanol tolerance in yeasts. , 1986, Critical reviews in microbiology.

[14]  N. Uden.  Temperature Profiles of Yeasts , 1985 .

[15]  Douglas Lloyd,et al.  Mitochondria of Microorganisms , 1976 .

[16]  P. Greenfield,et al.  Specific and non-specific inhibitory effects of ethanol on yeast growth , 1987 .

[17]  L. Ingram.  Adaptation of membrane lipids to alcohols , 1976, Journal of bacteriology.

[18]  C. Charpentier,et al.  Production and Tolerance of Ethanol in Relation to Phospholipid Fatty-acyl Composition in Saccharomyces cerevisiae NCYC 431 , 1982 .

[19]  H. Mcconnell,et al.  Lateral phase separations in membrane lipids and the mechanism of sugar transport in Escherichia coli. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[20]  D. Lloyd,et al.  The influence of oxygen and organic hydrogen acceotors on glycolytic dioxide production in Brettanomyces anomalus , 1988 .

[21]  C. Ough.  Fermentation Rates of Grape Juice. III. Effects of Initial Ethyl Alcohol, pH, and Fermentation Temperature , 1966, American Journal of Enology and Viticulture.

[22]  C. Leão,et al.  Effects of ethanol and other alkanols on passive proton influx in the yeast Saccharomyces cerevisiae. , 1984, Biochimica et biophysica acta.

[23]  L. Nover.  Expression of heat shock genes in homologous and heterologous systems , 1987 .

[24]  D Lloyd,et al.  Effects of alcohols on the respiration and fermentation of aerated suspensions of baker's yeast. , 1991, Journal of general microbiology.