Kinetic amperometric determination of hydrolase activity
暂无分享，去创建一个
Abstract Alkaline phosphatase is determined by using o -hydroxyphenyl or p -aminophenyl phosphate as substrate; for β-glucosidase, p -aminophenol β-D-glucoside is used. The products, p -aminophenol and catechol, are monitored amperometrically. The limit of determination for the hydrolase is about 2.5 × 10 -3 units ml -1 . The apparent Michaelis constants are given.
[1] G. Guilbault,et al. Preparation of immobilized cholinesterase for use in analytical chemistry. , 1965, Analytical chemistry.
[2] G. Guilbault,et al. An enzyme electrode based on immobilized arylsulfatase for the selective assay of sulfate ion. , 1976, Analytica chimica acta.