Presence of epidermal growth factor receptors and influence of epidermal growth factor on proliferation and aging in cultured smooth muscle cells

Epidermal growth factor (EGF) at nanomolar concentrations stimulated DNA synthesis in confluent, serum‐starved cultures of calf aorta and human uterine smooth muscle cells. Stimulation of DNA synthesis in lens epithelial cells was studied for comparison. L and D‐ascorbic acid potentiated the effect of serum and EGF on DNA synthesis in calf aorta cells. In contrast L‐ascorbic acid had minimal potentiating effect with serum and no effect with EFG present along with serum on DNA synthesis in human uterine smooth muscle and rabbit lens epithelial cells. EGF and ascorbic acid increased cell number when added to stationary phase cultures. Specific binding of 125I‐labelled EGF to smooth muscle cells was demonstrated. Receptor concentration in calf‐aorta smooth muscle cells was higher in dense cultures compared to sparse cultures. The time course of binding and dissociation of 125I‐labelled EGF was similar in “dense” and “sparse” cultures.

[1]  S. Singer,et al.  Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[2]  H. Herschman,et al.  Epidermal growth factor. Relationship between receptor regulation and mitogenesis in 3T3 cells. , 1978, The Journal of biological chemistry.

[3]  C. F. Fox,et al.  Molecular mechanism of mitogen action: processing of receptor induced by epidermal growth factor. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[4]  I. Pastan,et al.  Direct visualization of binding, aggregation, and internalization of insulin and epidermal growth factor on living fibroblastic cells. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[5]  P. Cuatrecasas,et al.  Fluorescent labeling of hormone receptors in viable cells: preparation and properties of highly fluorescent derivatives of epidermal growth factor and insulin. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[6]  K. Brown,et al.  Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by serum factors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[7]  H. Herschman,et al.  Specific radiolabeling of a cell surface receptor for epidermal growth factor. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[8]  B. Westermark.  Local starvation for epidermal growth factor cannot explain density-dependent inhibition of normal human glial cells. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[9]  H. Green,et al.  Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes , 1977, Nature.

[10]  G. Carpenter,et al.  125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts , 1976, The Journal of cell biology.

[11]  D. A. Dessouky.  Ultrastructural observations of the human uterine smooth muscle cells during gestation. , 1976, American journal of obstetrics and gynecology.

[12]  I. Pastan,et al.  Insulin receptors in normal and transformed fibroblasts: relationship to growth and transformation , 1976, Cell.

[13]  G. Carpenter,et al.  Human epidermal growth factor and the proliferation of human fibroblasts , 1976, Journal of cellular physiology.

[14]  H. Wolinsky,et al.  Endocytosis by vascular smooth muscle cells in vivo and in vitro. Roles or vesicles and lysosomes. , 1976, The American journal of pathology.

[15]  B. Westermark.  Density dependent proliferation of human glia cells stimulated by epidermal growth factor. , 1976, Biochemical and biophysical research communications.

[16]  K. Lembach.  Induction of human fibroblast proliferation by epidermal growth factor (EGF): enhancement by an EGF-binding arginine esterase and by ascorbate. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[17]  M. Hollenberg.  Receptors for insulin and epidermal growth factor: relation to synthesis of DNA in cultured rabbit lens epithelium. , 1975, Archives of biochemistry and biophysics.

[18]  H. Herschman,et al.  Initiation of 3T3 fibroblast cell division by epidermal growth factor , 1975, Journal of cellular physiology.

[19]  G. Balian,et al.  Collagen characterisation and cell transformation in human atherosclerosis , 1975, Nature.

[20]  G. Carpenter,et al.  Characterization of the binding of 125-I-labeled epidermal growth factor to human fibroblasts. , 1975, The Journal of biological chemistry.

[21]  P. Cuatrecasas,et al.  Insulin and epidermal growth factor. Human fibroblast receptors related to deoxyribonucleic acid synthesis and amino acid uptake. , 1975, The Journal of biological chemistry.

[22]  D. Gospodarowicz.  Purification of a fibroblast growth factor from bovine pituitary. , 1975, The Journal of biological chemistry.

[23]  G. Poste,et al.  Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities , 1975, The Journal of cell biology.

[24]  M. Haddox,et al.  Biologic regulation through opposing influences of cyclic GMP and cyclic AMP: the Yin Yang hypothesis. , 1975, Advances in cyclic nucleotide research.

[25]  J. Pawelek,et al.  Regulation of melanocyte stimulating hormone action at the receptor level: discontinuous binding of hormone to synchronized mouse melanoma cells during the cell cycle. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[26]  R. Revoltella,et al.  Unmasking of nerve growth factor membrane-specific binding sites in synchronized murine C 1300 neuroblastoma cells. , 1974, Experimental cell research.

[27]  L. Culp,et al.  Stimulation of 2-deoxyglucose uptake in growth-inhibited BALB-C 3T3 and revertant SV40-transformed 3T3 cells. , 1974, Experimental cell research.

[28]  P. Cuatrecasas,et al.  Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[29]  S. Cohen,et al.  Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization. , 1972, The Journal of biological chemistry.

[30]  T. Kanaseki,et al.  Role of Microexudate Carpet in Cell Division , 1972, Nature.

[31]  G. Summer,et al.  Collagen synthesis in human skin fibroblasts: effects of ascorbate, -ketoglutarate and ferrous ion on proline hydroxylation. , 1972, The Journal of nutrition.

[32]  D. Warhurst,et al.  Lysosomes, pH and the Anti-malarial Action of Chloroquine , 1972, Nature.

[33]  R. Ross,et al.  THE SMOOTH MUSCLE CELL I. In Vivo Synthesis of Connective Tissue Proteins , 1971 .

[34]  V. Cristofalo.  Metabolic Aspects of Aging in Diploid Human Cells , 1970 .

[35]  R. Wissler,et al.  Demonstration of smooth muscle cells in bovine arteriosclerosis an immunohistochemical study. , 1968, Journal of atherosclerosis research.

[36]  A. Daoud,et al.  Experimental atherosclerosis in rhesus monkeys. II. Cellular elements of proliferative lesions and possible role of cytoplasmic degeneration in pathogenesis as studied by electron microscopy. , 1967, Experimental and molecular pathology.

[37]  H. Green,et al.  Serum Albumin Supplemented Medium for Long Term Cultivation of Mammalian Fibroblast Strains.∗ , 1964, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[38]  S. Cohen,et al.  The stimulation of epidermal keratinization by a protein isolated from the submaxillary gland of the mouse. , 1963, The Journal of investigative dermatology.

[39]  S. Cohen,et al.  Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. , 1962, The Journal of biological chemistry.

[40]  L. Hayflick,et al.  The serial cultivation of human diploid cell strains. , 1961, Experimental cell research.

[41]  R. Perez-Tamayo,et al.  Studies on uterine collagen during pregnancy and puerperium. , 1961, Laboratory investigation; a journal of technical methods and pathology.