Antifungal susceptibility testing for dermatophytes isolated from clinical samples by broth dilution method in a tertiary care hospital

Purpose: To characterize antifungal susceptibility pattern of different species of dermatophytes isolated from clinical patients in a multispeciality hospital. Method: Micro broth dilution method was used to determine Minimum inhibitory concentration (MIC) for 129 positive samples with reference to CLSI document M38-A2. MIC was done against amphotericin B, fluconazole, ketoconazole, terbinafine, ciclopirox and griseofulvin. Results: The range of MIC was within the normal susceptibility range of the standard strain (Trichophyton mentagrophytes ATCC MYA – 4439) mentioned in CLSI document M-38 A2. Conclusion: No resistant strains were isolated in our study with reference to the standard testing method. None of the isolates have showed abnormal MIC range when compared to MIC of standard strain reported in literature. Hence, the isolates in our study are found to be susceptible to the antifungals used. However, future studies are required to evaluate the effect of drugs upon clinical response.