Effect of Mixed Culture Growth Conditions on the Cellular Fatty Acids of Streptococci (Analyzed by High Performance Liquid Chromatography)

Abstract : The effect of mixed culture growth conditions on the cellular fatty acids of bacteria were examined by high performance liquid chromatography. Streptococcus salivarius grown individually in mixed culture with Streptococcus mutans, Streptococcus sanguis, Staphylococcus aureus, and Escherichia coli showed no qualitative changes in cellular fatty acids when compared with cellular fatty acids detected in a pure culture of Streptococcus salivarius. Growth in mixed culture did affect the fatty acid profiles quantitatively, however. A pronounced quantitative shift in the ratio of unsaturated octadecanoic acid (18:0) to saturated octadecanoic 18:1 was observed when S. salivarius was grown in the presence of both S. mutans and S. sanguis. (Author)