Since procalcitonin (PCT) is a specific inflammation indicator of severe bacterial inflammation and fungal infection, it is of great significance to construct a sensitive and rapid microfluidic chip to detect PCT in clinical application. The design of micromixers using a lab-on-a-chip (LOC) device is the premise to realizing the adequate mixing of analytical samples and reagents and is an important measure to improve the accuracy and efficiency of determination. In this research study, we investigate the mixing characteristics of hyperbolic micromixers and explore the effects of different hyperbolic curvatures, different Reynolds numbers (Re) and different channel widths on the mixing performance of the micromixers. Then, an optimal micromixer was integrated into a microfluidic chip to fabricate a desirable hyperbolic microfluidic chip (DHMC) for the sensitive determination of inflammation marker PCT with a limit of detection (LOD) as low as 0.17 ng mL-1via a chemiluminescence signal, which can be used as a promising real-time platform for early clinical diagnosis.