论文信息 - A human T cell‐specific cDNA clone (YT16) encodes a protein with extensive homology to a family of protein‐tyrosine kinases

A human T cell‐specific cDNA clone (YT16) encodes a protein with extensive homology to a family of protein‐tyrosine kinases

We report here the isolation of a human T cell‐specific cDNA clone (YT16) that encodes a protein with extensive homology to a family of transformation and proliferation‐related protein‐tyrosine kinases (PTK). The message size of YT16 is 2.2 kb and it encodes a protein of 507 amino acids with a mol. mass of 57136 Daltons. The deduced protein sequence has an overall homology of about 50% to the viral oncogene products of src and yes. Around the PTK catalytic domain, the homologies increase to 70–80% between the deduced protein sequence of YT16 and those of the PTK viral oncogenes. Of special interest is the extensive homology between the deduced protein sequence of YT16 and that of a recently reported murine B and T lymphocyte‐specific PTK, lskT. It is possible that YT16 either encodes the human homologue of this murine gene, or a protein with similar function(s) in T cells.

[1] E. Jenkinson,et al. Embryology of the lymphoid system. , 1981, Progress in allergy.

[2] E. Krebs,et al. A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRA , 1985, Cell.

[3] J. Trevillyan,et al. High tyrosine-specific protein kinase activity in normal human peripheral blood lymphocytes. , 1985, Biochimica et biophysica acta.

[4] H. Hanafusa,et al. Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus , 1983, Cell.

[5] Tak W. Mak,et al. A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains , 1984, Nature.

[6] R. Fagard,et al. Tyrosine phosphorylation in human T lymphoma cells. , 1983, Biochemical and biophysical research communications.

[7] M. Yoshida,et al. Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virus , 1982, Nature.

[8] B. Sefton,et al. Expression of a new tyrosine protein kinase is stimulated by retrovirus promoter insertion , 1986, Nature.

[9] M. Minden,et al. Analysis of cDNA clones specific for human T cells and the alpha and beta chains of the T-cell receptor heterodimer from a human T-cell line. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[10] T. Hunter. Protein-Tryosine Kinases , 1985 .