Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells

The technique of DNA transfer by electroporation was investigated in an effort to evaluate its utility for the identification of developmentally controlled regulatory sequences. Transient and stable gene expression was detected in a variety of lymphoid cell lines subjected to electroporation. No correlation existed between the levels of chloramphenicol acetyltransferase (acetyl-CoA; chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28) expression and stable transfection frequency. In all lymphoid cell lines tested, the simian virus 40 early region was a better promoter than was the Rous sarcoma virus long terminal repeat.

[1]  J. Teissié,et al.  Electric field mediated transformation: isolation and characterization of a TK+ subclone. , 1985, Biochemical and biophysical research communications.

[2]  J. Miller,et al.  Specificity of mutations induced in transfected DNA by mammalian cells. , 1984, The EMBO journal.

[3]  P. Leder,et al.  Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[4]  S. Jhanwar,et al.  Establishment of a near-tetraploid B-cell lymphoma line with duplication of the 8;14 translocation. , 1984, Cancer genetics and cytogenetics.

[5]  T. Gudewicz,et al.  How damaged is the biologically active subpopulation of transfected DNA? , 1984, Molecular and cellular biology.

[6]  D. Stacey,et al.  Differences in intracellular DNA ligation after microinjection and transfection. , 1984, Molecular and cellular biology.

[7]  B. Howard,et al.  Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. , 1983, Nucleic acids research.

[8]  H. Luthman,et al.  High efficiency polyoma DNA transfection of chloroquine treated cells. , 1983, Nucleic acids research.

[9]  S. Morrison,et al.  Immunoglobulin gene expression in transformed lymphoid cells. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[10]  I. Pastan,et al.  The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[11]  B. Howard,et al.  Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells , 1982, Molecular and cellular biology.

[12]  E Neumann,et al.  Electric field mediated gene transfer. , 1982, Biochemical and biophysical research communications.

[13]  E. Neumann,et al.  Gene transfer into mouse lyoma cells by electroporation in high electric fields. , 1982, The EMBO journal.

[14]  W. Paul,et al.  Ia antigen-bearing B cell tumor lines can present protein antigen and alloantigen in a major histocompatibility complex-restricted fashion to antigen-reactive T cells , 1982, The Journal of experimental medicine.

[15]  R. Mulligan,et al.  Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[16]  H. Koprowski,et al.  Production of human hybridomas secreting antibodies to measles virus , 1980, Nature.

[17]  R. Mulligan,et al.  Expression of a bacterial gene in mammalian cells. , 1980, Science.

[18]  K. Rajewsky,et al.  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. , 1979, Journal of immunology.

[19]  G. Köhler,et al.  A better cell line for making hybridomas secreting specific antibodies , 1978, Nature.

[20]  R. Hyman,et al.  Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. , 1974, Journal of the National Cancer Institute.

[21]  J. Crowley,et al.  Electrical breakdown of bimolecular lipid membranes as an electromechanical instability. , 1973, Biophysical journal.

[22]  A. van der Eb,et al.  A new technique for the assay of infectivity of human adenovirus 5 DNA. , 1973, Virology.

[23]  C. Gorman.  Recombinant GenomesWhichExpress Chloramphenicol Acetyltransferase inMammalian Cells , 1982 .

[24]  F. Ruddle,et al.  Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[25]  P Berg,et al.  Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. , 1982, Journal of molecular and applied genetics.