Development and evaluation of a minicolumn assay for the detection of aflatoxin M1 in milk.

A practical field method for the chemiselective immobilization and detection of aflatoxin M1 in milk has been developed and is being marketed. In this new method, aflatoxin M1 is selectively adsorbed at the interface of a layer of neutral sand and a band of magnesium silicate (Florisil) packed in a glass minicolumn. Aflatoxin M1, at > or = .5 ppb in contaminated milk, can be easily visualized as a band of bright blue fluorescence. Briefly, raw or homogenized and pasteurized milk is diluted with water (1: 1, vol/vol) and passed through a C18 cartridge. Aflatoxin M1 is then partitioned by polarity, eluted with acetone-methylene chloride, and added to the minicolumn. The minicolumn is washed and viewed under long wave UV light. The limit of detection for this assay was .2 ppb, which was similar to the .3 ppb obtained using an immunoaffinity column, followed by minicolumn detection. The assay was accurate, rapid, easy to perform, and stable.

[1]  J. Chełkowski,et al.  Aflatoxine in Lebensmitteln , 1972 .

[2]  J. Hendricks,et al.  NEOPLASMS IN RAINBOW TROUT, A SENSITIVE ANIMAL MODEL FOR ENVIRONMENTAL CARCINOGENESIS * , 1977, Annals of the New York Academy of Sciences.

[3]  W. H. Butler.  Acute Toxicity of Aflatoxin B1 in Rats , 1964, British Journal of Cancer.

[4]  B. Ruebner,et al.  Carcinogenicity of dietary aflatoxin M1 in male Fischer rats compared to aflatoxin B1. , 1987, Cancer research.

[5]  E. H. Marth,et al.  Aflatoxin: Toxicity to Dairy Cattle and Occurrence in Milk and Milk Products - A Review. , 1982, Journal of food protection.

[6]  Brownie Cf,et al.  An epidemiological investigation associating aflatoxin M1 with milk production in dairy cattle. , 1988 .

[7]  T. Hansen.  Affinity Column Cleanup and Direct Fluorescence Measurement of Aflatoxin M1 in Raw Milk. , 1990, Journal of food protection.

[8]  R. Sinnhuber,et al.  Hepatic carcinogenesis of aflatoxin M1 in rainbow trout (Salmo gairdneri) and its enchancement by cyclopropene fatty acids. , 1974, Journal of the National Cancer Institute.

[9]  G. Wogan,et al.  Toxicity and biochemical and fine structural effects of synthetic aflatoxins M 1 and B 1 in rat liver. , 1971, Journal of the National Cancer Institute.

[10]  A. Hayes,et al.  Effect of aflatoxin B1, ochratoxin A and rubratoxin B on infant rats. , 1977, Food and cosmetics toxicology.

[11]  R. Adamson,et al.  Induction of osteogenic sarcomas and tumors of the hepatobiliary system in nonhuman primates with aflatoxin B1. , 1979, Cancer research.

[12]  B. Ruebner,et al.  Comparative hepatocarcinogenicity of aflatoxins B1 and M1 in the rat. , 1984, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[13]  R. Harvey,et al.  Effects on aflatoxin M1 residues in milk by addition of hydrated sodium calcium aluminosilicate to aflatoxin-contaminated diets of dairy cows. , 1991, American journal of veterinary research.

[14]  G. Wogan,et al.  Carcinogenic effects of low dietary levels of aflatoxin B1 in rats. , 1974, Food and cosmetics toxicology.

[15]  N. Takeda.  Determination of aflatoxin M1in milk by reversed-phase high-performance liquid chromatography , 1984 .