Effect of kirenol on cytokines in serum and apoptosis proteins expression in synoviocytes of adjuvant arthritis rats

Objective To investigate the effect of kirenol on serum concentration of IL-1β,TNF-α,and IL-6,as well as the expression of Bcl-2 and Fas-L proteins in synoviocytes of adjuvant arthritis(AA)rats.Methods AA Model of SD rats was made by means of Freund's complete adjuvant.Kirenol,Hydrocortisone,Aspirin and Cytoxan were administered for treatment,respectively.The levels of IL-1β,TNF-α,and IL-6 in serum of rats were assayed by means of ILISA.The immunohistochemical stain was used in synoviocytes and computer imagines analysis system was used to analyze the expression of Bcl-2 and Fas-L proteins.Results Compared with the normal group,the levels of IL-1β,TNF-α,and IL-6 in serum and the excessive expression of Bcl-2 in synoviocytes of AA model rats were increased,remarkably(P0.01,0.05),the expression of Fas-L was decreased significantly(P0.05);While compared with the model group,the levels of IL-1β,TNF-α,and IL-6 in serum of rats were significantly decreased by kirenol(P0.01,0.05),the protein expression of Bcl-2 in synoviocytes was down-regulated(P0.05),and the protein expression of Fas-L was increased(P0.05)by kirenol markedly.Conclusion Kirenol can decrease the levels of IL-1β,TNF-α,and IL-6 in serum of AA rats,down-regulate the excessive expression of Bcl-2 in synoviocytes,and promote the expression of Fas-L.