This paper presents an investigation of dynamic interaction of long suspension bridges with running trains. A three-dimensional finite element model is used to represent a long suspension bridge. Each 4-axle vehicle in a train is modelled by a 27-degrees-of-freedom dynamic system. The dynamic interaction between the bridge and train is realized through the contact forces between the wheels and track. By applying a mode superposition technique to the bridge only and taking the measured track irregularities as known quantities, the number of degrees of freedom (d.o.f.) the bridge-train system is significantly reduced and the coupled equations of motion are efficiently solved. The proposed formulation and the associated computer program are then applied to a real long suspension bridge carrying a railway within the bridge deck. The dynamic response of the bridge-train system and the derail and offload factors related to the running safety of the train are computed. The results show that the formulation presented in this paper can well predict dynamic behaviors of both bridge and train with reasonable computation efforts. Dynamic interaction between the long suspension bridge and train is not significant.