The Determination of the Blood Volume in Man with Evans Blue ("T-1824")

2. The carbon monoxide method of Haldane and Smith (1899) for the indirect determination of the total haemoglobin. A known volume of carbon monoxide is inspired by the subject and the concentration of the gas in combination with haemoglobin lri the circulating blood subsequently determined. Hence the total haemoglobin is computed. The chief objections to this Method are the technical difficulties involved and the considerable variation in the figures recorded by various investigators (Erlanger, *921 ; Graff and Clarke, 1931). 3The indirect determination of plasma volume by injecting Jnto the blood stream known amounts of various substances and