Multifunctional mesoporous silica-coated graphene nanosheet used for chemo-photothermal synergistic targeted therapy of glioma.

Current therapy of malignant glioma in clinic is unsatisfactory with poor patient compliance due to low therapeutic efficiency and strong systemic side effects. Herein, we combined chemo-photothermal targeted therapy of glioma within one novel multifunctional drug delivery system. A targeting peptide (IP)-modified mesoporous silica-coated graphene nanosheet (GSPI) was successfully synthesized and characterized, and first introduced to the drug delivery field. A doxorubicin (DOX)-loaded GSPI-based system (GSPID) showed heat-stimulative, pH-responsive, and sustained release properties. Cytotoxicity experiments demonstrated that combined therapy mediated the highest rate of death of glioma cells compared to that of single chemotherapy or photothermal therapy. Furthermore, the IP modification could significantly enhance the accumulation of GSPID within glioma cells. These findings provided an excellent drug delivery system for combined therapy of glioma due to the advanced chemo-photothermal synergistic targeted therapy and good drug release properties of GSPID, which could effectively avoid frequent and invasive dosing and improve patient compliance.

[1]  Debabrata Dash,et al.  Amine-modified graphene: thrombo-protective safer alternative to graphene oxide for biomedical applications. , 2012, ACS nano.

[2]  Baohong Liu,et al.  pH-controlled delivery of doxorubicin to cancer cells, based on small mesoporous carbon nanospheres. , 2012, Small.

[3]  Rongqin Huang,et al.  Targeted delivery of chlorotoxin-modified DNA-loaded nanoparticles to glioma via intravenous administration. , 2011, Biomaterials.

[4]  R. Puri,et al.  Novel anti–brain tumor cytotoxins specific for cancer cells , 1998, Nature Biotechnology.

[5]  Pedro M. Matos,et al.  Quantitative imaging of endosome acidification and single retrovirus fusion with distinct pools of early endosomes , 2012, Proceedings of the National Academy of Sciences.

[6]  H. Dai,et al.  Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy. , 2011, Journal of the American Chemical Society.

[7]  Wei Lu,et al.  Effects of photoacoustic imaging and photothermal ablation therapy mediated by targeted hollow gold nanospheres in an orthotopic mouse xenograft model of glioma. , 2011, Cancer research.

[8]  P. Morris,et al.  Medical oncology: Optimizing chemotherapy and radiotherapy for anaplastic glioma , 2010, Nature Reviews Clinical Oncology.

[9]  Feng Chen,et al.  An anti-ROS/hepatic fibrosis drug delivery system based on salvianolic acid B loaded mesoporous silica nanoparticles. , 2010, Biomaterials.

[10]  Zhuang Liu,et al.  Nano-graphene oxide for cellular imaging and drug delivery , 2008, Nano research.

[11]  Kai Yang,et al.  Graphene in mice: ultrahigh in vivo tumor uptake and efficient photothermal therapy. , 2010, Nano letters.

[12]  Guoliang Zhang,et al.  Deoxygenation of Exfoliated Graphite Oxide under Alkaline Conditions: A Green Route to Graphene Preparation , 2008 .

[13]  I-Wei Chen,et al.  Quantum‐Dot‐Tagged Reduced Graphene Oxide Nanocomposites for Bright Fluorescence Bioimaging and Photothermal Therapy Monitored In Situ , 2012, Advanced materials.

[14]  Kai Yang,et al.  In vivo pharmacokinetics, long-term biodistribution, and toxicology of PEGylated graphene in mice. , 2011, ACS nano.

[15]  C. Fan,et al.  Protein corona-mediated mitigation of cytotoxicity of graphene oxide. , 2011, ACS nano.

[16]  G. Ozin,et al.  Graphene oxide-periodic mesoporous silica sandwich nanocomposites with vertically oriented channels. , 2010, ACS nano.

[17]  Mauro Ferrari,et al.  Mesoporous silicon particles as a multistage delivery system for imaging and therapeutic applications. , 2008, Nature nanotechnology.

[18]  Rongqin Huang,et al.  Tumor cell targeted delivery by specific peptide-modified mesoporous silica nanoparticles , 2012 .

[19]  S. Shrivastava,et al.  Thrombus inducing property of atomically thin graphene oxide sheets. , 2011, ACS nano.

[20]  Emily S. Day,et al.  Vascular-targeted photothermal therapy of an orthotopic murine glioma model. , 2012, Nanomedicine.

[21]  H. Dai,et al.  Chemically Derived, Ultrasmooth Graphene Nanoribbon Semiconductors , 2008, Science.

[22]  Miqin Zhang,et al.  pH-Sensitive siRNA nanovector for targeted gene silencing and cytotoxic effect in cancer cells. , 2010, Molecular pharmaceutics.

[23]  Zhuang Liu,et al.  PEGylated nanographene oxide for delivery of water-insoluble cancer drugs. , 2008, Journal of the American Chemical Society.

[24]  Eric C. Holland,et al.  Targeting brain cancer: advances in the molecular pathology of malignant glioma and medulloblastoma , 2010, Nature Reviews Cancer.

[25]  P. Wen,et al.  Response criteria for glioma , 2008, Nature Clinical Practice Oncology.

[26]  P. Thordarson,et al.  Gram-scale production of graphene based on solvothermal synthesis and sonication. , 2009, Nature nanotechnology.

[27]  Z. Marković,et al.  In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. , 2011, Biomaterials.

[28]  Yuan Ping,et al.  Chitosan-functionalized graphene oxide as a nanocarrier for drug and gene delivery. , 2011, Small.

[29]  Jing Wang,et al.  Mesoporous Silica‐Coated Gold Nanorods as a Light‐Mediated Multifunctional Theranostic Platform for Cancer Treatment , 2012, Advanced materials.

[30]  Xiaogang Qu,et al.  Using Graphene Oxide High Near‐Infrared Absorbance for Photothermal Treatment of Alzheimer's Disease , 2012, Advanced materials.

[31]  Takako Sasaki,et al.  Inhibition of brain tumor growth by intravenous poly(β-l-malic acid) nanobioconjugate with pH-dependent drug release , 2010, Proceedings of the National Academy of Sciences.

[32]  Kai Yang,et al.  Nano-graphene in biomedicine: theranostic applications. , 2013, Chemical Society reviews.

[33]  Qing X. Yang,et al.  Efficacy of interleukin-13 receptor–targeted liposomal doxorubicin in the intracranial brain tumor model , 2009, Molecular Cancer Therapeutics.

[34]  G. Wallace,et al.  Processable aqueous dispersions of graphene nanosheets. , 2008, Nature nanotechnology.

[35]  Wenming Liu,et al.  Folate-decorated hybrid polymeric nanoparticles for chemically and physically combined paclitaxel loading and targeted delivery. , 2011, Biomacromolecules.

[36]  Agnes B Kane,et al.  Biological interactions of graphene-family nanomaterials: an interdisciplinary review. , 2012, Chemical research in toxicology.

[37]  Chen Jiang,et al.  Gene and doxorubicin co-delivery system for targeting therapy of glioma. , 2012, Biomaterials.