BCL6 alternative translocation breakpoint cluster region associated with follicular lymphoma grade 3B

Translocations involving band 3q27, affecting the major breakpoint region (MBR) of BCL6, are common in diffuse large B‐cell lymphomas (DLBCLs). Recent data suggest an alternative breakpoint cluster region (ABR) located between 245 and 285 kb 5′ of BCL6, which might be associated with Follicular Lymphoma (FL). Ten DLBCLs and 9 FLs grade 3B with cytogenetic rearrangements at 3q27 were studied by fluorescence in situ hybridization (FISH) to discriminate between breakpoints at the ABR and MBR. Eight DLBCLs contained a breakpoint in the MBR, and 6 FL grade 3B (FL3B) cases contained a breakpoint in the ABR. No specific chromosomal partners could be identified in both groups. Previously published data have suggested that FL3B cases with 3q27 aberrations are closely related to the majority of DLBCLs of germinal center cell origin. However, our findings suggest that the mechanism of 3q27 rearrangement in FL3B cases is similar to the mechanism in follicular lymphomas grade 1,2, and 3A cases. © 2005 Wiley‐Liss, Inc.

[1]  E. Haralambieva,et al.  Follicular lymphoma grade 3B includes 3 cytogenetically defined subgroups with primary t(14;18), 3q27, or other translocations: t(14;18) and 3q27 are mutually exclusive. , 2003, Blood.

[2]  E. Haralambieva,et al.  Detection of three common translocation breakpoints in non‐Hodgkin's lymphomas by fluorescence in situ hybridization on routine paraffin‐embedded tissue sections , 2002, The Journal of pathology.

[3]  R. Gascoyne,et al.  Alternative translocation breakpoint cluster region 5' to BCL-6 in B-cell non-Hodgkin's lymphoma. , 2002, Cancer research.

[4]  S. Barrans,et al.  Rearrangement of the BCL6 locus at 3q27 is an independent poor prognostic factor in nodal diffuse large B‐cell lymphoma , 2002, British journal of haematology.

[5]  P. Rao,et al.  The t(2;3)(q21;q27) translocation in non-Hodgkin's lymphoma displays BCL6 mutations in the 5′ regulatory region and chromosomal breakpoints distant from the gene , 1998, Oncogene.

[6]  S. Iida,et al.  Heterologous Promoters Fused to BCL6 by Chromosomal Translocations Affecting Band 3q27 Cause Its Deregulated Expression During B-Cell Differentiation , 1998 .

[7]  R S Chaganti,et al.  BCL-6, a POZ/zinc-finger protein, is a sequence-specific transcriptional repressor. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[8]  L. Staudt,et al.  Transcriptional repression by the proto-oncogene BCL-6. , 1996, Oncogene.

[9]  D. Leprince,et al.  The LAZ3/BCL6 oncogene encodes a sequence-specific transcriptional inhibitor: a novel function for the BTB/POZ domain as an autonomous repressing domain. , 1995, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[10]  R. Chaganti,et al.  Chromosomal translocations cause deregulated BCL6 expression by promoter substitution in B cell lymphoma. , 1995, The EMBO journal.

[11]  D. Leprince,et al.  The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins. , 1995, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[12]  S. Mori,et al.  BCL-6 gene product, a 92- to 98-kD nuclear phosphoprotein, is highly expressed in germinal center B cells and their neoplastic counterparts. , 1995, Blood.

[13]  A. Rossi,et al.  LAZ3 rearrangements in non-Hodgkin's lymphoma: correlation with histology, immunophenotype, karyotype, and clinical outcome in 217 patients. , 1994, Blood.

[14]  K Offit,et al.  Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma. , 1993, Science.

[15]  H. Tilly,et al.  LAZ3, a novel zinc–finger encoding gene, is disrupted by recurring chromosome 3q27 translocations in human lymphomas , 1993, Nature Genetics.

[16]  R. Espinosa,et al.  Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[17]  N. Aoki,et al.  Gene Involved in the 3 q 27 Translocation Associated With B-Cell Lymphoma , BCLS , Encodes a Kruppel-Like Zinc-Finger Protein , 2002 .

[18]  C. Bastard,et al.  FISH analysis with a YAC probe improves detection of LAZ3/BCL6 rearrangement in non-Hodgkin's lymphoma. , 2000, The hematology journal : the official journal of the European Haematology Association.

[19]  S. Iida,et al.  Heterologous promoters fused to BCL6 by chromosomal translocations affecting band 3q27 cause its deregulated expression during B-cell differentiation. , 1998, Blood.

[20]  N. Aoki,et al.  Gene involved in the 3q27 translocation associated with B-cell lymphoma, BCL5, encodes a Krüppel-like zinc-finger protein. , 1994, Blood.