Impact of a yogurt matrix and cell microencapsulation on the survival of Lactobacillus reuteri in three in vitro gastric digestion procedures.

The goal of this study was to assess the interaction between microencapsulation and a yogurt food matrix on the survival of Lactobacillus reuteri NCIMB 30242 in four different in vitro systems that simulate a gastric environment. The four systems were: United States Pharmacopeia (USP) solutions, a static two-step (STS) procedure which included simulated food ingredients, a constantly dynamic digestion procedure (IViDiS), as well a multi-step dynamic digestion scheme (S'IViDiS). The pH profiles of the various procedures varied between systems with acidity levels being: USP > STS > IViDiS = S'IVIDiS. Addition of a food matrix increased the pH in all systems except for the USP methodology. Microencapsulation in alginate-based gels was effective in protecting the cells in model solutions when no food ingredients were present. The stability of the probiotic culture in the in vitro gastric environments was enhanced when (1) yoghurt or simulated food ingredient were present in the medium in sufficient quantity, (2) pH was higher. The procedure-comparison data of this study will be helpful in interpreting the literature with respect to viable counts of probiotics obtained from different static or dynamic in vitro gastric systems.

[1]  J. Scher,et al.  Encapsulation of Lactobacillus rhamnosus GG in microparticles: Influence of casein to whey protein ratio on bacterial survival during digestion , 2013 .

[2]  Zhen-Xing Tang,et al.  Encapsulation of probiotic Lactobacillus bulgaricus in alginate–milk microspheres and evaluation of the survival in simulated gastrointestinal conditions , 2013 .

[3]  G. Nychas,et al.  Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. , 2013, Food microbiology.

[4]  G. Paoli,et al.  How does Listeria monocytogenes combat acid conditions? , 2013, Canadian journal of microbiology.

[5]  T. Masud,et al.  Recent Trends and Applications of Encapsulating Materials for Probiotic Stability , 2013, Critical reviews in food science and nutrition.

[6]  S. Baines,et al.  In vitro analysis of gastrointestinal tolerance and intestinal cell adhesion of probiotics in goat's milk ice cream and yogurt , 2012 .

[7]  A. Brodkorb,et al.  Application of whey protein micro-bead coatings for enhanced strength and probiotic protection during fruit juice storage and gastric incubation , 2012, Journal of microencapsulation.

[8]  S. Prakash,et al.  Cholesterol lowering and inhibition of sterol absorption by Lactobacillus reuteri NCIMB 30242: a randomized controlled trial , 2012, European Journal of Clinical Nutrition.

[9]  S. Prakash,et al.  Evaluation of clinical safety and tolerance of a Lactobacillus reuteri NCIMB 30242 supplement capsule: a randomized control trial. , 2012, Regulatory toxicology and pharmacology : RTP.

[10]  S. Prakash,et al.  Evaluation of safety and tolerance of microencapsulated Lactobacillus reuteri NCIMB 30242 in a yogurt formulation: a randomized, placebo-controlled, double-blind study. , 2012, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[11]  U. Kulozik,et al.  Microencapsulation of Probiotic Cells for Food Applications , 2012, Critical reviews in food science and nutrition.

[12]  D. Poncelet,et al.  Stability of lactobacilli encapsulated in various microbial polymers. , 2012, Journal of bioscience and bioengineering.

[13]  H. Ejtahed,et al.  Factors Influencing Probiotic Survival in Ice Cream: A Review , 2012 .

[14]  T. Tompkins,et al.  The impact of meals on a probiotic during transit through a model of the human upper gastrointestinal tract. , 2011, Beneficial microbes.

[15]  Seizo Yamashita,et al.  Avaliação do tempo de trânsito esofágico pelo ultrassom: influência do gênero e índice de massa corpórea , 2011 .

[16]  Satya Prakash,et al.  Cholesterol-lowering efficacy of a microencapsulated bile salt hydrolase-active Lactobacillus reuteri NCIMB 30242 yoghurt formulation in hypercholesterolaemic adults , 2011, British Journal of Nutrition.

[17]  R. P. Ross,et al.  Recommendations for the viability assessment of probiotics as concentrated cultures and in food matrices. , 2011, International journal of food microbiology.

[18]  Sung Je Lee,et al.  Functional properties of free and encapsulated Lactobacillus reuteri DPC16 during and after passage through a simulated gastrointestinal tract , 2011, World Journal of Microbiology and Biotechnology.

[19]  D. Mcclements,et al.  In vitro human digestion models for food applications , 2011 .

[20]  T. Vandamme,et al.  In vitro effects of pH, bile salts and enzymes on the release and viability of encapsulated Lactobacillus plantarum strains in a gastrointestinal tract model , 2011 .

[21]  A. Abghari,et al.  Nonfermented ice cream as a carrier for Lactobacillus acidophilus and Lactobacillus rhamnosus , 2011 .

[22]  S. Tynkkynen,et al.  Persistence of probiotic strains in the gastrointestinal tract when administered as capsules, yoghurt, or cheese. , 2010, International journal of food microbiology.

[23]  M. Marco,et al.  Food formats for effective delivery of probiotics. , 2010, Annual review of food science and technology.

[24]  Y. Rivera-Espinoza,et al.  Non-dairy probiotic products. , 2010, Food microbiology.

[25]  N. Shah,et al.  An improved method of microencapsulation of probiotic bacteria for their stability in acidic and bile conditions during storage. , 2009, Journal of food science.

[26]  P. Sherman,et al.  Unraveling mechanisms of action of probiotics. , 2009, Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition.

[27]  Satya Prakash,et al.  Microencapsulated bile salt hydrolase producing Lactobacillus reuteri for oral targeted delivery in the gastrointestinal tract , 2008, Applied Microbiology and Biotechnology.

[28]  S. Tynkkynen,et al.  In vitro and in vivo gastrointestinal survival, antibiotic susceptibility and genetic identification of Propionibacterium freudenreichii ssp. shermanii JS , 2008 .

[29]  W. Verstraete,et al.  Comparison of five in vitro digestion models to in vivo experimental results: Lead bioaccessibility in the human gastrointestinal tract , 2007, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[30]  R. P. Ross,et al.  Survival of Probiotic Lactobacilli in Acidic Environments Is Enhanced in the Presence of Metabolizable Sugars , 2005, Applied and Environmental Microbiology.

[31]  Y. Arcand,et al.  A dynamic model that simulates the human upper gastrointestinal tract for the study of probiotics. , 2005, International journal of food microbiology.

[32]  A. Oomen,et al.  Applicability of an in vitro digestion model in assessing the bioaccessibility of mycotoxins from food. , 2005, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[33]  N. Read,et al.  Investigation into the role of cephalic stimulation of acid secretion on gastric emptying and appetite following a soup meal using the gastric acid inhibitor omeprazole , 2004, Appetite.

[34]  J. Dressman,et al.  Upper Gastrointestinal (GI) pH in Young, Healthy Men and Women , 1990, Pharmaceutical Research.

[35]  S. Salminen,et al.  The Health Effects of Cultured Milk Products with Viable and Non-viable Bacteria , 1998 .

[36]  G. Young,et al.  A bifunctional urease enhances survival of pathogenic Yersinia enterocolitica and Morganella morganii at low pH , 1996, Journal of bacteriology.

[37]  M. Martini,et al.  Lactose digestion by yogurt beta-galactosidase: influence of pH and microbial cell integrity. , 1987, The American journal of clinical nutrition.