Bioencapsulation within synthetic polymers (Part 1): sol-gel encapsulated biologicals.

Since its inception a decade ago, sol-gel encapsulation has opened up an intriguing new way to immobilize biological materials. An array of substances, including catalytic antibodies, DNA, RNA, antigens, live bacterial, fungal, plant and animal cells and whole protozoa, have been encapsulated in silica, metal-oxide, organosiloxane and hybrid sol-gel polymers. The advantages of these 'living ceramics' might give them applications as optical and electrochemical sensors, diagnostic devices, catalysts, and even bioartificial organs. With rapid advances in sol-gel precursors, nanoengineered polymers, encapsulation protocols and fabrication methods, this technology promises to revolutionize bioimmobilization.

[1]  M. Reetz,et al.  Efficient Heterogeneous Biocatalysts by Entrapment of Lipases in Hydrophobic Sol–Gel Materials , 1995 .

[2]  A. Ballesteros,et al.  Novel Sol‐Gel Matrices for the Immobilization of Enzymes a , 1996, Annals of the New York Academy of Sciences.

[3]  P. Tanev,et al.  Biomimetic Templating of Porous Lamellar Silicas by Vesicular Surfactant Assemblies , 1996, Science.

[4]  Bradley F. Chmelka,et al.  MESOCELLULAR SILICEOUS FOAMS WITH UNIFORMLY SIZED CELLS AND WINDOWS , 1999 .

[5]  D. Pine,et al.  Monolithic Mesoporous Silica Templated by Microemulsion Liquid Crystals , 2000 .

[6]  J. Li,et al.  Silica sol-gel immobilized amperometric enzyme electrode for peroxide determination in the organic phase , 1998 .

[7]  David Avnir,et al.  Organic Chemistry within Ceramic Matrixes: Doped Sol-Gel Materials , 1995 .

[8]  J. Brennan.  Using Intrinsic Fluorescence to Investigate Proteins Entrapped in Sol-Gel Derived Materials , 1999 .

[9]  David Avnir,et al.  Enzymes and Other Proteins Entrapped in Sol-Gel Materials , 1994 .

[10]  J. Kumar,et al.  Phycobiliproteins encapsulated in sol-gel glass , 1996 .

[11]  Juan Li,et al.  Renewable silica sol-gel derived carbon composite based glucose biosensor , 1999 .

[12]  J. Zink,et al.  Synthesis conditions for encapsulating cytochrome c and catalase in SiO2 sol-gel materials , 1996 .

[13]  A F Collings,et al.  Biosensors: recent advances , 1997 .

[14]  M. Smyth,et al.  Development of a sol-gel enzyme inhibition-based amperometric biosensor for cyanide , 1997 .

[15]  J. Livage,et al.  Antibody-antigen reactions in porous sol-gel matrices , 1997 .

[16]  R. Chatterton,et al.  Entrapment of a highly specific antiprogesterone antiserum using polysiloxane copolymers. , 1984, Biochimica et biophysica acta.

[17]  S. Bharathi.  Sol-gel-derived nanocrystalline gold-silicate composite biosensor , 1998 .

[18]  C. Webb,et al.  Studies in viable cell immobilization , 1996 .

[19]  D. Avnir,et al.  Sol-gel entrapment of monoclonal anti-atrazine antibodies , 1996 .

[20]  J. Zink,et al.  Nicotinamide Adenine Dinucleotide Phosphate Fluorescence and Absorption Monitoring of Enzymic Activity in Silicate Sol-Gels for Chemical Sensing Applications , 1995 .

[21]  Gordon F. Bickerstaff.  Immobilization of Enzymes and Cells , 1996 .

[22]  M. Reetz.  Entrapment of biocatalysts in hydrophobic sol‐gel materials for use in organic chemistry , 1997 .

[23]  Sergei Braun,et al.  Immobilization in Alginate-Silicate Sol-Gel Matrix Protects β-Glucosidase Against Thermal and Chemical Denaturation , 1995, Bio/Technology.

[24]  Jacques Livage,et al.  Optical detection of parasitic protozoa in sol-gel matrices , 1994, Optics & Photonics.

[25]  S. Dong,et al.  Amperometric enzyme electrode for the determination of hydrogen peroxide based on sol–gel/hydrogel composite film , 2000 .

[26]  S. Yang,et al.  Microfabricated glucose biosensor with glucose oxidase entrapped in sol-gel matrix. , 1998, Talanta.

[27]  Bruce Dunn,et al.  Sol-gel encapsulation methods for biosensors , 1994 .

[28]  M. Antonietti,et al.  Mesoporous Silica from Lyotropic Liquid Crystal Polymer Templates. , 1998, Angewandte Chemie.

[29]  G. Carturan,et al.  Production of Valuable Drugs from Plant Cells Immobilized by Hybrid Sol-Gel SiO2 , 1998 .

[30]  J. Zink,et al.  Enzymatic activity of oxalate oxidase and kinetic measurements by optical methods in transparent sol-gel monoliths , 1996 .

[31]  Weinberg,et al.  Combinatorial approaches to materials discovery. , 1999, Trends in biotechnology.

[32]  J. Livage,et al.  Immunoassays in sol-gel matrices , 1996 .

[33]  Jian Lin,et al.  Oxidation of dibenzothiophene catalyzed by heme-containing enzymes encapsulated in sol-gel glass , 1994, Applied biochemistry and biotechnology.

[34]  D. Avnir,et al.  Properties of trypsin and of acid phosphatase immobilized in sol‐gel glass matrices , 1992, Biotechnology and applied biochemistry.

[35]  J. Zink,et al.  Synthesis of Protein-Doped Sol-Gel SiO2 Thin Films: Evidence for Rotational Mobility of Encapsulated Cytochrome c , 1995 .

[36]  J. E. Mark.  The Sol–Gel Route to Inorganic‐Organic Composites , 1996 .

[37]  Y. Murakami,et al.  Immobilization of a thermostable enzyme using a sol-gel preparation method , 1996 .

[38]  †‡§ and Iqbal Gill,et al.  Encapsulation of Biologicals within Silicate, Siloxane, and Hybrid Sol−Gel Polymers: An Efficient and Generic Approach , 1998 .

[39]  M. Smyth,et al.  Sol–gel-based amperometric glucose biosensor incorporating an osmium redox polymer as mediator , 1996 .

[40]  Modeeparampil N. Kamalasanan,et al.  A novel protocol to entrap active urease in a tetraethoxysilane-derived sol-gel thin-film architecture , 1994 .

[41]  T. Yao,et al.  Amperometric biosensor with a composite membrane of sol-gel derived enzyme film and electrochemically generated poly(1,2-diaminobenzene) film. , 1998, Biosensors & bioelectronics.

[42]  H. Weetall,et al.  Retention of bacteriorhodopsin activity in dried sol-gel glass. , 1996, Biosensors & bioelectronics.

[43]  S. Dirè,et al.  Entrapment of viable microorganisms by SiO2 sol-gel layers on glass surfaces: trapping, catalytic performance and immobilization durability of Saccharomyces cerevisiae. , 1993, Journal of Biotechnology.

[44]  P. Pandey,et al.  Studies on Glucose Biosensors Based on Nonmediated and Mediated Electrochemical Oxidation of Reduced Glucose Oxidase Encapsulated Within Organically Modified Sol‐Gel Glasses , 1999 .

[45]  D. A. Russell,et al.  Optical Biosensing of Nitrate Ions Using a Sol–Gel Immobilized Nitrate Reductase , 1997 .

[46]  N. Kröger,et al.  Polycationic peptides from diatom biosilica that direct silica nanosphere formation. , 1999, Science.

[47]  M Ensor,et al.  Bacterial biosensors for monitoring toxic metals. , 1997, Trends in biotechnology.

[48]  M. Smyth,et al.  Development of a sol–gel based amperometric biosensor for the determination of phenolics† , 1998 .

[49]  R. Šimkus,et al.  Laccase Containing Sol-Gel Based Optical Biosensors , 1996 .

[50]  U. Narang,et al.  Removal of ribonucleases from solution using an inhibitor-based sol-gel-derived Biogel , 1995 .

[51]  P. Audebert,et al.  Electrochemical probing of the activity of glucose oxidase embedded sol-gel matrixes , 1993 .

[52]  O. Lev,et al.  Sol-gel derived, ferrocenyl-modified silicate-graphite composite electrode: Wiring of glucose oxidase , 1996 .

[53]  N. Kostić,et al.  Kinetics of Photoinduced Electron-Transfer Reactions within Sol-Gel Silica Glass Doped with Zinc Cytochrome c. Study of Electrostatic Effects in Confined Liquids , 1997 .

[54]  M. Reetz,et al.  In situ fixation of lipase-containing hydrophobic sol–gel materials on sintered glass—highly efficient heterogeneous biocatalysts , 1996 .

[55]  M. Reiss,et al.  Monitoring of Environmental Processes with Biosensors , 2001 .

[56]  G G Guilbault,et al.  Non-invasive biosensors in clinical analysis. , 1995, Biosensors & bioelectronics.

[57]  Joseph Wang,et al.  Tailoring the macroporosity and performance of sol-gel derived carbon composite glucose sensors , 1997 .

[58]  G. Kuncová,et al.  Lipase immobilized in organic-inorganic matrics , 1997 .

[59]  D. Avnir,et al.  Biochemical aspects of sol-gel science and technology : a special issue of the Journal of sol-gel science and technology , 1996 .

[60]  H. Böttcher,et al.  Biosensor properties of glucose oxidase immobilized within SiO2 gels , 1997 .

[61]  Joseph Wang,et al.  Self-Assembled Silica Gel Networks , 1998 .

[62]  M. Reetz,et al.  Efficient immobilization of lipases by entrapment in hydrophobic sol‐gel materials , 2000, Biotechnology and bioengineering.

[63]  A. N. Díaz,et al.  Sol-gel horseradish peroxidase biosensor for hydrogen peroxide detection by chemiluminescence , 1998 .

[64]  J. Friedman,et al.  Impeded rotation of a protein in a sol-gel matrix , 1999 .

[65]  S. Sampath,et al.  Renewable, reagentless glucose sensor based on a redox modified enzyme and carbon‐silica composite , 1996 .

[66]  B. Dave,et al.  Enzymatic Conversion of Carbon Dioxide to Methanol: Enhanced Methanol Production in Silica Sol−Gel Matrices , 1999 .

[67]  N. Kostić,et al.  Photoinduced electron transfer from zinc cytochrome c to cationic ruthenium(III) complexes within silica glass prepared by the sol-gel method , 1997 .

[68]  Juan Li,et al.  Silica sol–gel immobilized amperometric biosensor for the determination of phenolic compounds , 1998 .

[69]  D. Poncelet,et al.  Immobilization of cells for application in the food industry. , 1994, Critical reviews in biotechnology.

[70]  D. A. Russell,et al.  Optical Biosensing of Gaseous Nitric Oxide Using Spin-Coated Sol−Gel Thin Films , 1997 .

[71]  Stephen Mann,et al.  Sol−Gel Synthesis of Organized Matter , 1997 .

[72]  V. Tvarozek.  Microsystem Technology in Biosensors , 1998 .

[73]  D. Russell,et al.  Calcium biosensing with a sol-gel immobilized photoprotein. , 1996, The Analyst.

[74]  Joseph T. Hupp,et al.  Sol-gel-encapsulated alcohol dehydrogenase as a versatile, environmentally stabilized sensor for alcohols and aldehydes , 1998 .

[75]  S. Upadhyay,et al.  A New Glucose Biosensor Based on Sandwich Configuration of Organically Modified Sol-Gel Glass , 1999 .

[76]  R. Brent,et al.  Purification of sheep immunoglobin G using protein A trapped in sol-gel glass. , 1992, Analytical biochemistry.

[77]  Jacques Livage.  Bioactivity in sol-gel glasses , 1996 .

[78]  Bradley F. Chmelka,et al.  Magnetic Field Alignment of Ordered Silicate-Surfactant Composites and Mesoporous Silica , 1997 .

[79]  S. Sampath,et al.  Sol-gel derived ceramic-carbon enzyme electrodes: Glucose oxidase as a test case , 1996 .

[80]  R. Mandelbaum,et al.  Entrapment of atrazine chlorohydrolase in sol-gel glass matrix , 1998 .

[81]  T. Park.  Amperometric Determination of Hydrogen Peroxide by Utilizing a Sol-Gel-Derived Biosensor Incorporating an Osmium Redox Polymer as Mediator , 1999 .

[82]  R. Takigiku,et al.  Online Microreactors/Capillary Electrophoresis/Mass Spectrometry for the Analysis of Proteins and Peptides , 1995 .

[83]  L. Hench,et al.  The sol-gel process , 1990 .

[84]  S. Cosnier,et al.  Sol-gel derived composite materials for the construction of oxidase/peroxidase mediatorless biosensors , 1997 .

[85]  Immobilization and Characterization of Lactate Dehydrogenase on TEOS Derived Sol-Gel Films , 1997 .

[86]  J. Wang,et al.  Sol-gel-derived gold composite electrodes. , 1997, Analytical chemistry.

[87]  S. Glazier,et al.  A fluorescence assay for dissolved oxygen using sol-gel encapsulated myoglobin and an analogy to the inner filter effect , 1996 .

[88]  J S Valentine,et al.  Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method. , 1992, Science.

[89]  M. Smyth,et al.  Sol-gel based amperometric biosensor incorporating an osmium redox polymer as mediator for detection of L-lactate. , 1997, Talanta.

[90]  G A Baker,et al.  Kinetics and thermodynamics of free flavins and the flavin-based redox active site within glucose oxidase dissolved in solution or sequestered within a sol-gel-derived glass. , 1999, Analytical chemistry.

[91]  D. Ronen,et al.  Atrazine degradation by Pseudomonas strain ADP entrapped in sol-gel glass , 1996 .

[92]  P. Pandey,et al.  Reversal in the kinetics of the M state decay of D96N bacteriorhodopsin: probing of enzyme catalyzed reactions , 1996 .

[93]  Chris W. Brown,et al.  Sol-gel glass as a matrix for chemical and biochemical sensing , 1997 .

[94]  Karlheinz Drauz,et al.  Enzyme Catalysis in Organic Synthesis , 1995 .

[95]  H. Weetall.  D96N mutant bacteriorhodopsin immobilized in sol-gel glass characterization , 1994 .

[96]  George M. Whitesides,et al.  Enzymes in Synthetic Organic Chemistry , 1994 .

[97]  E. Keinan,et al.  An efficient sol-gel reactor for antibody-catalyzed transformations , 1997 .

[98]  S. Dong,et al.  Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium. , 1999, Analytical chemistry.

[99]  Börje Sellergren,et al.  Use of silane monomers for molecular imprinting and enzyme entrapment in polysiloxane-coated porous silica , 1985 .

[100]  J Wang,et al.  Screen-printable sol-gel enzyme-containing carbon inks. , 1996, Analytical chemistry.

[101]  R. Armon,et al.  Entrapment of parathion hydrolase from Pseudomonas spp. in sol-gel glass , 1996 .

[102]  R. Campostrini,et al.  Immobilization of plant cells in hybrid sol-gel materials , 1996 .

[103]  C. Dosoretz,et al.  Sol-gel applications in environmental biotechnology. , 1996, Journal of biotechnology.

[104]  David Avnir,et al.  Biochemically active sol-gel glasses: The trapping of enzymes ☆ , 1990 .

[105]  B. Dave,et al.  Selective Intake and Release of Proteins by Organically-Modified Silica Sol−Gels , 1998 .

[106]  R. Mandelbaum,et al.  Entrapment of atrazine-degrading enzymes in sol-gel glass , 1996 .

[107]  S. Saavedra,et al.  Spectroscopic characterization of albumin and myoglobin entrapped in bulk sol-gel glasses , 1994 .

[108]  D. Avnir,et al.  Sol-gel-entrapped cholinesterases: a microtiter plate method for monitoring anti-cholinesterase compounds , 1998 .

[109]  J. Zink,et al.  Spectroscopic Determination of Cholinesterase Activity and Inhibition in Sol-Gel Media , 1997 .

[110]  U. Schubert,et al.  Aerogels-Airy Materials: Chemistry, Structure, and Properties. , 1998, Angewandte Chemie.

[111]  Bruce Dunn,et al.  Probes of Pore Environment and Molecule-Matrix Interactions in Sol-Gel Materials , 1997 .