Dielectric measurement to monitor the growth and the physiological states of biological cells

Measurement of capacitance, also referred to as dielectric permittivity, is a new method of estimating the concentration of cells, monitoring the growth and detecting the physiological changes during the cultivation of organisms in various bioprocess. Several types of biological cells were studied, namely; Saccharomyces cerevisiae, Escherichia coli, Perilla frutescens (plant cells) and AFP-27 hybridoma cells. Generally, a linear correlation between cell capacitance (C) and other biomass measurement technique such as optical density (OD) and dry weight (DW) was obtained using the different types of cell suspension. Therefore, this method could be used to monitor the growth of the organism during the active growth. It could be conveniently used to make a rapid estimate of the cell concentration such as in plant cell suspension culture. The capacitance sensor could also be designed to be installed and autoclaved in-situ in a bioreactor and used for on-line monitoring of cell growth. On the other hand, distinct deviations in the capacitance value were observed in relation with the growth stage of the organism. This was observed in all the organisms studied but the type of deviation depends on the physiology of the organism. This variation in cell capacitance showed the possibility of using this method as a means to indicate changes in the physiological state of cells during cultivation. This capability would be very useful in designing control strategies that would depend on the physiological states in the bioprocess.

[1]  G. Markx,et al.  Dielectric spectroscopy as a novel and convenient tool for the study of the shear sensitivity of plant cells in suspension culture. , 1991, Journal of biotechnology.

[2]  Toshiomi Yoshida,et al.  Loss of cell viability by excessive synthesis of a specific mRNA in phenylalanine production , 1987 .

[3]  G. Hunt,et al.  On‐line analysis of avermectin fermentation cell growth kinetics in an industrial pilot plant , 1989, Biotechnology and bioengineering.

[4]  J. Gier.  Permeability barriers formed by membrane lipids , 1992 .

[5]  H. Hashimoto,et al.  Origin of changes in electrical impedance during the growth and fermentation process of yeast in batch culture , 1989, Biotechnology and bioengineering.

[6]  B. Martinez,et al.  Characteristics of Batch Suspension Cultures of Preconditioned Coleus blumei Cells: Sucrose Effect , 1993 .

[7]  P. Lord,et al.  Dependency of size of Saccharomyces cerevisiae cells on growth rate , 1979, Journal of bacteriology.

[8]  Christopher L. Davey,et al.  On the dielectric properties of cell suspensions at high volume fractions , 1992 .

[9]  Argyrios Margaritis,et al.  Kinetics of sterilization of Lactobacillus brevis cells by the application of high voltage pulses , 1992, Biotechnology and bioengineering.

[10]  D. Thomas,et al.  Causes of conductance change in yeast cultures. , 1992, The Journal of applied bacteriology.

[11]  K. K. Frame,et al.  Cell volume measurement as an estimation of mammalian cell biomass , 1990, Biotechnology and bioengineering.

[12]  A. E. Humphrey,et al.  Estimation of Fermentation Biomass Concentration by Measuring Culture Fluorescence , 1978, Applied and environmental microbiology.

[13]  J. E. Prenosil,et al.  On-line monitoring of cell growth in plant tissue cultures by conductometry , 1989 .

[14]  A. Zaritsky,et al.  Rate maintenance of cell division in Escherichia coli B/r: analysis of a simple nutritional shift-down , 1992, Journal of bacteriology.

[15]  Toshiomi Yoshida,et al.  On-Line Monitoring of Representative Structural Variables in Fed-Batch Cultivation of Recombinant Escherichia coli for Phenylalanine Production , 1990 .

[16]  Christopher L. Davey,et al.  On the audio- and radio-frequency dielectric behaviour of anchorage-independent, mouse L929-derived LS fibroblasts , 1988 .

[17]  D. Kell,et al.  Real-time monitoring of the accretion of Rhizopus oligosporus biomass during the solid-substrate tempe fermentation , 1991, World journal of microbiology & biotechnology.

[18]  Toshiomi Yoshida,et al.  Physiologically motivated strategies for control of the fed-batch cultivation of recombinant Escherichia coli for phenylalanine production , 1991 .

[19]  Y. Takahara,et al.  On-Line Monitoring of Cell Concentrations during Yeast Cultivation by Dielectric Measurements , 1991 .

[20]  L. Alberghina,et al.  Flow Cytometry and Cell Cycle Kinetics in Continuous and Fed‐Batch Fermentations of Budding Yeast , 1991, Biotechnology progress.

[21]  K B Konstantinov,et al.  On‐line monitoring of hybridoma cell growth using a laser turbidity sensor , 1992, Biotechnology and bioengineering.

[22]  Ken Mishima,et al.  On-line monitoring of cell concentrations by dielectric measurements , 1991 .

[23]  Douglas B. Kell,et al.  Dielectric permittivity of microbial suspensions at radio frequencies: a novel method for the real-time estimation of microbial biomass , 1987 .

[24]  K Konstantinov,et al.  Physiological state control of fermentation processes , 1989, Biotechnology and bioengineering.

[25]  Toshiomi Yoshida,et al.  Rheological characteristics of cell suspension and cell culture of Perilla frutescens , 1992, Biotechnology and bioengineering.

[26]  L. Alberghina,et al.  Involvement of a cell size control mechanism in the induction and maintenance of oscillations in continuous cultures of budding yeast , 1990, Biotechnology and bioengineering.